Analysis of drug use in community empty-nest and non-empty-nest elderly hypertension patients / 药学实践杂志

Objective To explore the needs of community pharmacy services in elderly hypertensive patients in the community, especially empty-nest elderly patients. Methods Elderly hypertensive patients living in Ouyang street were randomly selected and divided into empty-nest and non-empty-nest groups by cluster random sampling method. The basic information of the respondents, the frequency of hypertension monitoring, the taking of hypertensive drugs, and the taking of other drugs were compared and analyzed. Results In term of “blood pressure monitoring frequency”, the daily pressure measurement of the empty-nest group and the non-empty-nest group accounted for 33.6% and 19.3%, respectively. There was significant difference between the two groups (P<0.05).In term of “the varieties of hypertension drugs” and taking 3 kinds of hypertension drugs at the same times, the empty-nest group accounted for 28.8% and the non-empty-nest group accounted for 16.7%, and the difference between the two groups was significant (P<0.05)；In term of “the varieties of drugs” and taking 1-2 kinds of Chinese patent drugs at the same time, the empty-nest group accounted for 39.6% and the non-empty-nest group accounted for 26.0% , and the difference between the two groups was significant (P<0.05)。Conclusion Community elderly patients with hypertension, especially empty-nest elderly patients have an urgent need for community pharmacy services，Community pharmacy services personnel should provide personalized and targeted medication education and guidance to elderly patients, especially empty-nest elderly patients, to promote the rational drug use in elderly patients.

Association between thyroid hormones and visceral fat area in the patients with type 2 diabetes / 中华内分泌代谢杂志

Objective:To explore the association between thyroid hormones and visceral fat area(VFA) in the patients with type 2 diabetes.Methods:A total of 729 patients with type 2 diabetes, who joined National Metabolic Management Center(MMC) through Ningbo First Hospital from March, 2018 to July, 2019, were enrolled in this study. Blood tests were taken to assess their thyroid hormones and biochemical indexes. VFA and subcutaneous fat area(SFA) were obtained through bioelectrical impedance analysis. Statistics were later analyzed by Spearman correlation analysis and multivariate linear regression. Results:Spearman correlation analysis showed that VFA were positively correlated with T 3、FT 3( r=0.225 and 0.211, P<0.01), and VFA were inversely correlated with FT 4( r=-0.112, P<0.01). No correlation was found between VFA and either of T 4 or TSH( P>0.05). Multivariate linear regression analysis indicated that VFA was independently correlated with FT 4 and FT 3( P<0.01). An increment of 1 pmol/L in FT 4 was associated with 1.759 cm 2 increase in VFA, and increment of 1 pmol/L in FT 3 was associated with 3.256 cm 2 decrease in VFA. Conclusion:VFA in patients with type 2 diabetes are correlated with FT 3 and FT 4.

Association between thyroid hormones and visceral fat area in the patients with type 2 diabetes / 中华内分泌代谢杂志

Objective@#To explore the association between thyroid hormones and visceral fat area(VFA) in the patients with type 2 diabetes.@*Methods@#A total of 729 patients with type 2 diabetes, who joined National Metabolic Management Center(MMC) through Ningbo First Hospital from March, 2018 to July, 2019, were enrolled in this study. Blood tests were taken to assess their thyroid hormones and biochemical indexes. VFA and subcutaneous fat area(SFA) were obtained through bioelectrical impedance analysis. Statistics were later analyzed by Spearman correlation analysis and multivariate linear regression.@*Results@#Spearman correlation analysis showed that VFA were positively correlated with T3、FT3(r=0.225 and 0.211, P<0.01), and VFA were inversely correlated with FT4(r=-0.112, P<0.01). No correlation was found between VFA and either of T4 or TSH(P>0.05). Multivariate linear regression analysis indicated that VFA was independently correlated with FT4 and FT3(P<0.01). An increment of 1 pmol/L in FT4 was associated with 1.759 cm2 increase in VFA, and increment of 1 pmol/L in FT3 was associated with 3.256 cm2 decrease in VFA.@*Conclusion@#VFA in patients with type 2 diabetes are correlated with FT3 and FT4.

Efficient Screen of Toxic Herbal Medicine in Zhuang Gu Guan Jie Pill Based on Zebrafish Model / 世界科学技术-中医药现代化

This study aimed at efficiently screening out the toxic herbal medicines in Zhuang Gu Guan Jie pill (ZGGJ) based on the zebrafish model.Zebrafish embryos at 1 day post fertilization (dpf) were administered by ZGGJ and the water decoction (WD) and ethanol extract (EE) of 12 herbal drugs in it with various concentrations.The number of embryo deaths after the administration was recorded at 2-6 dpf and 2-8 dpf,respectively.Embryonic micro-morphology of zebrafish (3 dpf) was observed,LC50 value at 6 dpf was calculated by SPSS16.0.As a result,both the WD and EE of ZGGJ,fructus psoraleae,radix dipsaci,olibanum,myrrh,radix angelicae pubescentis and aucklandiae radix,and the EE of Caulis Spatholobo and Rhizoma Cibotii presented high organ toxicity to the juvenile zebrafish,featuring yolk sac swelling,deforming,blackening,indicating hepatotoxicity in the zebrafish.Their LC50 value at 6 dpf was from 33.2 μg·mL-1 to 392.7 μg·mL-1 (LD50 value was converted to crude drug.The same bellow.).The WD and EE of Epimedii Folium at 1,500 μg· mL-1 led to yolk sac swelling and blackening.The LC50 at 6 dpf varied at 1,100-1,350 μg·mL-1,showing a certain toxicity of Epimedii Folium.The WD of Caulis Spatholobo and Cibotii Rhizoma and both WD and EE of Rhizoma drynariae,taxilli herba and prepared rehmanniae radix didn' t change the toxicity to the organs in zebrafish.LC50 value were over 1,500 μg·mL-1,presenting favorable safety.In conclusion,it was demonstrated that the zebrafish model efficientlyidentified the toxicity of different herbal medicines from ZGGJ and efficiently screened out the potential toxicants in vivo with the advantages of real-time,dynamic and large scale for traditional Chinese medical compounds.

Effect of extract ethanol and water decoction of Psoralea corylifolia L.on bone development and their toxicities in zebrafish / 中国药理学与毒理学杂志

OBJECTIVE To investigate the bone development activity and differences in safety of ethanol extract (EE) and water decoction (WD) of Psoralea corylifolia L.efficiently.METHODS Zebrafish larvae were co-exposed to prednisolone 25 μmol· L-1 and different concentrations of EE and WD (0.1,1.0,10 and 100 mg crude drug· L-1),and etidronate disodium (ED) 30 mg·L-1.All these groups were incubated at 28.5℃ until 9 dpf.The medium solution was changed every other day.Zebrafish skeleton at 9 dpf was stained with alizarin red and inspected under an optical microscope,in addition,the death toll and organ toxicity of zebrafish were also observed.The mRNA expression of osteoprotegrin (OPG) and receptor activator of NF-κB ligand (RANKL) in 9 dpf zebrafish were determined with fluorescence quantitative PCR.Zebrafish embryos (1 dpf) were exposed to various concentrations of EE (10,20,30,35,40,50 and 60 mg crude drug· L-1),WD (10,50,100,125,150,175,200 and 500 mg crude drug· L-1),psoralen (12.5,25.0,50.0,100.0,200.0 and 400.0 μmol·L-1) and bakuchiol (1,5,10,25 and 50 μmol· L-1).Embryonic morphology of zebrafish (3 dpf) was inspected with an optical microscope and the death toll of embryos or larvale was counted from 2 dpf to 9 dpf and LC50was calculated.Components of EE and WD ware analyzed by HPLC method.RESULTS Both EE (0.1 mg crude drug· L-1) and WD (1.0 mg crude drug· L-1) groups could increase the staining area and optical density values of zebrafish skeleton compared with prednisolone group (P＜0.01),indicating the increase in bone mineralization;the OPG mRNA expression in both EE and WD (1.0 mg crude drug· L-1) groups increased,while the RANKL mRNA expression decreased (P＜0.01) and the ratio of OPG/RANKL improved obviously (P＜0.01).Embryos exposed to EE,WD,psoralen and bakuchiol showed swelling of the heart and yalk sac,and decrease in GOT.The LC50 of WD and psoralen was 5～8 and 5～21 times that EE and bakuchiol,respectively.The composition and relative content of EE and WD also varied considerably.CONCLUSION Bone development activity and toxicity of EE are both stronger than those of WD.The lipid soluble characteristic components of Psoralea corylifolia L.,may be critical components of toxicity.

Study on sociopsychological factors in patients with occupational hand injury / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the role of sociopsychological factors in occupational hand injury and to provide a reference for intervention measures.</p><p><b>METHODS</b>The sociopsychological factors in 150 patients with occupational hand injury who were admitted to the Department of Hand Surgery from January to June in 2014 and 150 pair-matched normal controls were surveyed using self-made questionnaire and Life Event Scale (LES). A comparative study was carried out using t test, χ² test, and conditional logistic regression analysis.</p><p><b>RESULTS</b>The demographic characteristics, such as length of service, education level, and marital status, showed significant differences between the two groups (P<0.05) ; the LES total score and values of positive life events and negative life events also showed significant differences between the study group and control group (P<0.01) ; single-factor and multi-factor conditional logistic regression analyses showed that length of service, marital status, and negative events score were statistically significant variables (P<0.01).</p><p><b>CONCLUSION</b>Sociopsychological factors play an important role in the occurrence of occupational hand injury.</p>

SNP microarray analysis of retention abortion chorionic villus / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To compare villus cell culture and karyotype analysis with single nucleotide polymorphism (SNP) microarray technology for the detection of chorionic villus chromosome in patients with retention of abortion.</p><p><b>METHODS</b>Forty cases were analyzed with the two methods.</p><p><b>RESULTS</b>Chorionic villus culturing was successful in 29 cases, among which 10 were found to have an abnormal karyotypes. For the SNP microarray analysis, all 40 cases were successful, among which 16 were shown to have an abnormal molecular karyotype.</p><p><b>CONCLUSION</b>SNP microarray technology is highly accurate and specific, which is particularly suitable for the detection of chromosomal deletions or duplications, uniparental disomy, low-percentage mosaicism and other chromosomal abnormalities. It has provided an effective supplement to the conventional chorionic villus culture and karyotype analysis.</p>

Long-term oncological outcomes after laparoscopic versus abdominal radical hysterectomy in stage Ⅰa2-Ⅱa2 cervical cancer: a matched cohort study / 中华妇产科杂志

Objective To investigate the long-term oncological outcomes of laparoscopic radical hysterectomy (LRH) plus lymph node dissection (LND) and abdominal radical hysterectomy (ARH) plus LND for patients with stage Ⅰ a2-Ⅱ a2 cervical cancer.Methods A retrospective review of stage Ⅰ a2-Ⅱ a2 cervical cancer patients who underwent LRH + LND (n=372) and ARH + LND (n=434) at the First Affiliated Hospital of Sun Yat-sen University from Jan.2005 to Aug.2013 was performed.Individual patient matching was performed by the risk factors for recurrence [tumor size,lymph vascular space invasion (LVSI),depth of cervical stromal invasion,lymph node metastasis,parametrialinvolvement,and resection margin involvement] between two groups.After matched,a total of 203 patient pairs (LRH-ARH) were enrolled.The survival data,surgery data,intraoperative and postoperative complications were compared between the two groups.To assess the prognosis factors,the univariate and multivariate Cox's proportional hazards modelanalysis were conducted.Stratified analysis was performed based on the independent prognosis factors to investigate the survival data between the two surgery groups.Results (1) Surgery data:The operating time [(239±44) vs (270±42) minutes],estimated blood loss [(210± 129) vs (428±320) ml],the duration of bowel motility return [(2.0±0.8) vs (3.0± 1.6) days] and hospital stay [(11 ±6) vs (13±6) days] in the LRH group were significantly shorter than those in ARH group (all P＜0.01).(2) Intraoperative and postoperative complications:The intraoperative complications rate was similar betweentwo groups [6.4％(13/203) vs 6.9％(14/203),P=1.000].The rate of postoperative complications (excluded bladder dysfunction) in the LRH group were significantly lower than those in the ARH group [9.4％ (19/203) vs 20.2％ (41/203),P=0.002].While there was no significant difference in the rates of bladder dysfunction between two groups [36.5％ (74/203) vs 37.4％ (76/203),P=0.910].(3) Recurrence and survival data:There was no significant difference in the recurrence rates between the LRH group and ARH groups [7.9％ (16/203) vs 9.4％ (19/203),P=0.850].There were similar 5-year recurrence-free survival (RFS;92.1％ vs 91.1％,P=0.790) and 5-year overall survival (OS;93.7％ vs 96.1％,P=0.900).(4) Prognosis factor:In univariate analysis,the results showed that tumor size,International Federation of Gynecology and Obstetrics (FIGO) stage,adjuvant therapy,LVSI,stromal invasion,parametrium invasion,pelvic lymph node metastasis,and para-aortic lymph node metastasis were significantly associated with poor prognosis (all P＜0.01).However,age,body mass index (BMI),surgery type,histological type,grade were not significantly associated with poor prognosis (all P＞0.05).The multivariate analysis results,showed that tumor size,pelvic lymph node metastasis,and para-aortic lymph node metastasis were significantly associated with poor prognosis (all P＜0.01).Stratified analysis showed that,even in patients with tumor size ＞4 cm,pelvic lymph node metastasis positive,and para-aortic lymph node metastasis positive in all subgroups,there were not significant difference for the estimated 5-year RFS and 5-year OS between LRH and ARH group (all P＞0.05).Conclusion For patients with stage Ⅰ a2-Ⅱ a2 cervical cancer,LRH plus lymph node dissection is an oncologically safe and surgical feasible alternative to ARH.

Reference intervals for liver function tests in normal pregnant women in the last trimester of pregnancy / 国际检验医学杂志

Objective To explore changes of serum levels of liver function indicators and their reference intervals in pregnant women in the last trimester of pregnancy ,and to analyse its clinical significance .Methods From July 2013 to June 2014 ,a total of 4 659 cases of pregnant women in the last trimester of pregnancy and 3 089 healthy non‐pregnant women were enrolled in this stud‐y .Serum levels of alanine aminotransferase(ALT) ,aspartatre transaminase(AST) ,total protein(TP) ,albumin(Alb) ,pre‐albumin (PA) ,total bilrubin(TBIL) ,direct bilrubin(DBIL) ,indirect bilrubin(IBIL) ,γ‐glutamyl transpeptidase(GGT)and mitochondrial as‐partate aminotransferase(m‐AST) were determined by fully automatic biochemical analyzer .Results Compared with healthy non‐pregnant women ,serum levels of ALT ,AST ,TP ,Alb ,PA ,TBIL ,DBIL ,IBIL and GGT were significantly decreased ,while serum le‐vers of ALP ,m‐AST and TBA were significantly increased in pregnant women in the last trimester of pregnancy ,there were statis‐tically significant differences(P<0 .05) .Conclusion This study suggested that the liver functions of pregnant women were signifi‐cantly different from healthy non‐pregnant women .Each clinical laboratory should establish reference intervals of liver function indi‐cators for pregnant women at each stage of pregnancy ,so as to timely ,correctly and reasonably evaluate their liver functions and material nutrition status ,and provide better antenatal care services .

Application analysis of critical value reporting system in a obstetrics and gynecology hospital / 国际检验医学杂志

Objective To analyze the clinical application value of the critical value reporting system.Methods The data record-ings of the critical values reported by the clinical laboratory department from Nov.2013 to May.2014 were inquired through the clinical laboratory information management system(LIS).The incidence rate of the critical values,receipt time of the critical values, items and department distribution were analyzed.Results A total of 1856 items of critical value were reported.The occurrence rate of the critical values was 0.31%.The top 5 of critical value numbers according to the item distribution were white blood cells (46.28%),total bilirubin (16.92%),D-dimer (9.81%),hemoglobin(8.51%)and platelets (8.03%).According to the depart-ments distribution,the top 3 departments with maximal reported critical values were the neonatal department (37.12%),followed by the department of gynecological oncology(23.33%)and the obstetrical department (21.17%).Conclusion The critical value reporting system plays an important role in the obstetrics and gynecology hospital.It should be evaluated and analyzed regularly.

Clinical significance of expression of enhancer of zeste homolog 2 and 53 in cervical squamous cell carcinoma and intraepithelial neoplasia / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the expression and clinical significance of enhancer of zeste homolog 2 (EZH2) and p53 proteins in cervical squamous cell carcinoma (SCC) and cervical intraepithelial neoplasia (CIN).</p><p><b>METHODS</b>The expression and distribution of EZH2 and p53 were determined with reference to clinicopathological features and patient survival. 168 cervical SCC, 19 CINII, 35 CINIII patients and 30 normal control cases were collected for immunohistochemical analysis.</p><p><b>RESULTS</b>The expression of EZH2 in the normal cervix, CIN and SCC was 6.7% (2/30), 37.0% (20/54) and 75.6% (127/168), respectively, with a significant difference between them (P < 0.05). The expression of p53 was 3.3% (1/30), 20.4% (11/54) and 39.3% (66/168), respectively, also (P < 0.05). In the 168 SCC cases, the positive rate of EZH2 in the cases with lymph node metastasis was 82.9%, and that of p53 was 45.7%; the positive rate of EZH2/p53 protein expression in the cases with negative lymph nodes was 70.4%, and that of p53 was 34.7%, with a significant difference between the two subgroups (P < 0.05). Among the 143 followed-up SCC patients, the EZH2(+) p53(+) cases had a progression-free survival of (51.3 ± 3.8) months, that of EZH2(+) or p53(+) cases was (66.1 ± 2.0) months, and that of EZH2(-) p53(-) cases was (71.3 ± 1.9) months, with a very significant difference among the three subgroups (P < 0.001). The overall survival times of EZH2(-) p53(-), EZH2(+) or p53(+), and EZH(2+) p53(+) cases were (72.9 ± 1.1), (68.6 ± 1.8), and (57.4 ± 3.4) months, respectively, with a significant difference among the three subgroups (P < 0.001). The multivariate analysis showed that EZH2 expression, lymph node metastasis and tumor staging were independent prognostic factors.</p><p><b>CONCLUSIONS</b>Both EZH2 and p53 proteins may play important roles in the occurrence and development of cervical squamous cell carcinoma. There is a close relationship between the expression of both EZH2 and p53 proteins and the prognosis of SCC patients.</p>

Establishment of archives of Schistosoma japonicum antibody indirect hemag-glutination tests / 中国血吸虫病防治杂志

Objective To establish written and electronic archives of Schistosoma japonicum antibody indirect hemagglutina-tion(IHA)tests. Methods In the process of schistosomiasis screening by IHA,the written records,electronic records,and se-rum sample bank were combined to make comprehensive archives. Results The S. japonicum antibody IHA test archives can pre-serve the schistosomiasis screening data in the long term and even can trace the source of experiments,and the operation was sim-ple. Conclusion The archives of S. japonicum antibody IHA tests are simple and useful,and worth of popularization.

Bone morphogenetic proteins and tumors / 国际肿瘤学杂志

Bone morphogenetic proteins (BMPs) are multi-functional growth factors,they are found not only inducer of new bone formation,but also have critical roles in cell growth,differentiation,migration,apoptosis,embryogenesis and organogenesis.Studies show that BMPs are closely related to tumorigenesis and metastasis,which have important practical values in tumour therapy.

Expressions of human β defensin-2 in eutopic and ectopic endometrium of women with adenomyosis / 中国综合临床

Objective To investigate the expression of human β defensin-2(hβD-2)in the eutopic and ectopic endometrial tissue in patients with adenomyosis and in women with normal endometrium.Methods Twenty-five hysteromyoma patients with adenomyosis(AM) and 25 hysteromyoma patients without endometriosis (EMS) were selected and divided into three groups:AM ectopic endometrium group,AM eutopic endometrium group and control group( endometrial tissue in patients with hysteromyoma).The level of mRNA expressions of hβD-2,interleukin-1β ( IL-1β ),interleukin-6 ( IL-6 ) and tumor necrosis factor-α (TNF-α) was investigated quantitatively using Real Time PCR (RT-PCR) and the corresponding protein level was detected by immunohistochemistry.Results Comparing the expression of hβD-2,IL-1β,IL-6,TNF-α genes among the three groups,there was no significant difference between ectopic endometrium group and eutopic endometrium group and there was also no significant difference between eutopic endometrium group and the control group.( P ＞ 0.05 ).The expression of hβD-2 and IL-1β were 0.0320 (0.0095 ～ 0.0690 ) and 0.0427 ( 0.0038 ～ 0.0975 ) in the ectopic endometrium group,and they were 0.0034(0.0025 ～0.0424) and 0.0080(0.0040 ～0.0251 ) in the control group,respectively.They were both significantly higher in ectopic endometrium group than in the control group (P ＜ 0.05 ).In the ectopic endometrium group hβD-2 expression was positively correlated with the level of TNF-α ( r =0.857,P =0.014 ),and it had no correlation with both IL-1β and IL-6 ( r =0.750,P =0.052 ; r =0.464,P =0.464; respectively)Conclusion HβD-2 might not play an important role in the formation of adenomyosis.It may be related to no significant up-regulation of inflammatory factors in ectopic lesion tissue.

Clinical significance of thrombospondin 4 expression in gastric carcinoma / 解剖学报

Objective The aim of this study was to measure quantitatively the TSP-4 mRNA expression and its significance in gastric carcinoma was evaluated by correlating its expression with clinicopathological features, microvessel density(MVD) and MMP-9.Methods Eighty-two patients with gastric carcinoma were recruited in this study. TSP-4 mRNA expression in gastric carcinoma and adjacent tissue was detected by real-time PCR. CD34 and MMP-9 protein expression were examined by immunohistochemistry. MVD was determined according to CD34-positive tubular structures. Results The expression level of TSP-4mRNA in gastric carcinoma was obviously higher than those of adjacent tissue(P=0.03) and it was associated with tumor size, histological type, lymph node metastasis, MVD and MMP-9(P=0.002, P=0.031, P=0.014,r＝0.67 P<0.01, P=0.008),but not sex, age and depth of invasion. (P=0.53,P=0.57,P=0.15).Conclusion TSP-4 may play an important role in occurrence and progression of gastric carcinoma and that the effect of TSP-4 on tumor growth and metastasis may be exerted through regulation of angiogenesis and MMP-9 expression in gastric carcinoma.

Influencing factors and prevention of vascular crisis after digital replantation / 中华护理杂志

Objective To explore the influencing factors and preventive measures of vascular crisis after digital replantation.Methods The clinical data of 726 patients with digital replantation from Jan 2006 to Jun 2008 were retrospectively investigated.Among which,120 cases suffered from vascular crisis after digital replantation.The possible influencing factors of vascular crisis were analyzed by Logistic regression analysis.Results Logistic regression analysis showed that sex,age,smoking history,causes of injury,ischemia duration,and surface of division were the predictors of vascular crisis after digital replantation.Conclusion Appropriate preventive measures aimed at the influencing factors should be taken to prevent and reduce vascular crisis after digital replantation,and thereby to increase the survive rate of replanted digits.

The clinical value of hypophysin injection into the cornua uterus and mesosalpinx during laparoscopic stripping of ovarian endometrioma / 中国医师进修杂志

Objective To evaluate the safety and the feasibility of hypophysin injection for hemostasis during laparoscopic stripping of ovarian endometrioma.Methods Retrospective analysis of 86 cases with ovarian endometrionm.Forty-two patients with prophylactic dilute hypophysin injection into cornua uterus and mesosalpinx(study group),and 44 patients without vasopressin(control group).The operative time,intraoperative blood loss,the highest postoperative temperature,postoperative stay and the rate of recurrence were compared.Results The operative time in the study group and the control group were(51.24±22.58)min and(67.02±25.14)min,the intraoperative blood loss were(42.16±26.10)ml and (68.23±28.21)ml,respectively.There was significantly different between two groups(P＜0.01).The rates of recurrence in the study group and the control group were 4.76% and 11.36%,respectively.But it was no significantly different between two groups(P＞0.05).Both the highest postoperative temperature and postoperative stay was no difference between two groups(P＞0.05).Conclusions Hypophysin can reduce the operative time and intraoperative blood loss during laparoscopic stripping of ovarian endometrioma,and it is possible to reduce the rate of recurrence.It is a safe,feasible,cheapand convenient method,and worth using in clinic.