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Objective To study the effect of itraconazole/IFN-gamma on IFN-? and IL-10 in nude mice and mice and clinical significance. Methods Systemic infection of animals that induced by abdominal injection of Candida albicans and Cryptococcus neoformans, Expression of IFN-? and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA).Results After treated with Itraconazole and IFN-gamma, the levels of IFN-gamma were increased and ones of IL-10 were decreased significantly. Conclusions Itraconazole and IFN-gamma may play key roles in the immune function of those animals.
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Objective: To establish a curve of dosage gradient-liver signal intensity of superparamagnetic iron oxide (SPIO) in normal rats and to find an appropriate dose for enhancement. Methods: Seventy-two SD rats, 4 rats a group at random, underwent MR enhancement with 0, 2, 5, 8, 10, 12, 15, 20, 30, 40, 50, 60, 70, 80, 100, 140, 210, 280 μmol/kg SPIO respectively after plane examination. The signal-to-noise ratio of liver was measured and a curve of dosage gradient-liver signal intensity was made. Results: (1)With the increase of SPIO dose, the signal intensity of both T1 and T2 weighted images of liver declined. (2)T2 weighted images was more sensitive than T1 weighted images in small dose, the ED50 of T1 weighted was 8 μmol/kg, and the ED50 of T2 weighted was 5 μmol/kg. (3)When the dose was greater than 15 μmol/kg, the signal intensity of T1 weighted images declined more rapidly than T2 weighted, the effect of enhanced T1 weighted images resembled T2 weighted ones, and its images was with more fine resolution. (4)At the dose of 40 μmol/kg, the signal intensity of T1 weighted images approached the background noise, and at the dose of 15 μmol/kg, the signal intensity of T2 weighted images approached the background noise. Conclusion: Good effect of T1 and T2 enhanced MR imaging can be acquired at the dose of 20-10 μmol/kg SPIO respectively, the best contrast-to-noise ratio is found on T2 weighted enhanced MR image.
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Objective: To investigate the pathological changes of human hepatocellular carcinoma (HCC) after continuous passaging in nude mice. Methods: The mice model of HCC SMMU-LTMN were continuously observed for 20 years (1987-2007) . The subcutaneously transplanted carcinoma had been passaged for 228 generations. The pathological data of abdominally transplanted HCC, orthotopically transplanted HCC in nude mice, and orthotopically transplanted HCC in NOD-SCID mice were recorded. The pathological studies were conducted by light microscope, electron microscope, image analysis, chromosomal analysis, and measurement of alpha-fetoprotein (AFP) in peripheral blood. Results: (1) The local invasion and metastasis of of tumors were present in all the above 4 models for a long time. The local invasion rate and the pulmonary metastasis rate of subcutaneously transplanted tumors were 59.70% (40/67) and 37.10% (23/62), respectively. The pulmonary metastasis rate of abdominally transplanted tumors was 59.02%(36/61). The intra-hepatic and pulmonary metastasis rate of the othotopically transplanted tumors were 18.18%(4/22) and 31.82% (7/22), respectively. The pulmonary metastasis rate of HCC in NOD-SCID mice was 53.85%. (2) The tissue structure and the differentiation of the 10th generation tumor cells was similar to those of primary HCC, with grade 2 differentiation and coarse trabecular pattern as the main characteristics. From the 11th generation to the 228th generation, the main characteristics of tumor cells were grade 3 differentiation and lump pattern. Electron microscope also showed worse differentiation. (3)The AFP level was 92 500 ?g/L in cells before the 32th generation; it decreased to 6 729?g/L from the 33th-130th generation cells; and the level of the 220th generation was 1 000-5 000 ?g/L.(4)The DNA contents had a wide distribution (from 2c to 6c) in abdominally transplanted tumors and the pulmonary metastatic tumors; the mean DNA index in the former tumors (2.60?0.20) was wider than the that in the latter (2.10?0.26) . (5)From the 55th generation to 206th generation, it was found that tumor cells had integrated into the chromosome of the nude mice. Conclusion: The subcutaneously transplanted HCC in nude mice can be stably expressed for 20 years, with no change in the local invasion and metastasis ability of HCC. The differentiation of the tumor cells worsenes and the AFP level is decreased in the blood; some chromosome of tumor cells integrate into the chromosome of nude mice, which may be related to the internal environment of nude mice and the multi-potential differentiation of the tumor cells.
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Thirty one male Wistar rats weighing 130-150 g were continuously fed with aflatoxin B1 (AFB1) for 45 weeks.Each rat received 3.89mg of AFB1 crystals in total close.Three treated rats and one control ret were killed at the 8th, 9th, 11th, 12th, 13th, 14th and 1.3th month, respectively, and seven rats died naturaly after the 17th month.Histological sections of the liver were stained with hematoxylin-eosin.Morphological changes of 28 rats' liver tissues stained with enzyme-labelled immuno-fetoprotein, and ultrastructural chinges of the liver specimens from 15 rats were observed .Histologicall ', the main manifestations of liver cells included hydratoid degeneration, eosinophilic degeneration, basophilic change.adipose degeneration and focal necrosis.The answer reaction showed oval cell and bilbary proliferation, hepatocellular regeneration and inflammatory cell infiltration.All of the above changes were chronic non-specific toxic hepatitis. The ultrastructural changes showed nucleolar uniformation, nucleoid body formation, mitochondria swelling and matrix uniformation, and vesicle formation and focal necrosis. 85.71 % 24/28)of the alpha-fetoprotein positive hepatocytes were found in host liver tissues.The AFP positive material mostly distributed in all of the plasma of liver cells.AFP positive hepatocytes were dispersed or focal in the liver tissue. This result suggests that the liver cell atavism mav be the toxic effect of aflatoxin B1.
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Observations on the histopathology of malignant granuloma in 5 cases were mace One of them was of the generalized type,with widespread pathological changes,confirmed by autopsy.The other 4 were of the localized type and treated by radiating.Two of them died and autopsy examinations were made whereas the clinical symptoms subsided in the other two cases.In one case there was exacerbation of symptoms after local injection of penicillin.One case revealed nodular reticulosis in the spleen.The histopathologic features of the disease are quite in accord with these of allergic,necrotizing and granulomatous vasculitis.They may occur locally on the nose,face and lungs and may also become widespread in the whole body.Some focal histological changes after radiotherapy are also discussed in the paper.
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Observations on 142 cases of noncirrhotic hepatocellular carcinoma (NCH) (135 surgical operating specimens, 8 autopic cases) were made. 50 cases of cirrhotic hepatocellular carcinoma (CH) (42 surgical operating specimens, 8 autopic cases) were taken as the control. The results were as follows:(1)In both groups, the hepatocellular carcinoma (HCC) had a similar background of liver diseases. 51.41% of the cases of hepatitis B and 48.59% of the cases of"orcein negative hepatitis" were associated with NCH,while 58.00% of the former and 42.00% of the latter were associated with CH. In both groups in the cases of HCC were included 5 cases of primary multiple carcinoma. We considered that pathogenesis of HCC were synthetic factors. It was assumed that NCH is due to weak carcinogen, hepatic parenchyma is lightly destroyed, and as the human body has hypersusceptibility to HCC, the precancerous stage is short and cancer occurs early.(2)In 80.99% of the NCH cases and 100% of the CH cases was found proliferation of oval cells. The difference between the two groups of HCC was statistically significant.The proliferation may be a repairing reaction. Owing to the action of car-cinosen, the poorly differentiated HCC may be derived from the disturbances of oval cells.(3)In 29.57% of the cases NCH and 70.00% of the cases CH was found hepar tocyte dysplasia, and the difference between the two groups of HCC statistically significant. It was indicated that dysplasia may be precancerous disoders.Oval cell proliferation and hepatocyte dysplasia of the NCH group are less than those of the CH group.It is suggested that liver tissues of the NCH group are slightly damaged, hence the incidence of NCH is lower than that of CH.
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The results of the rat hepatocellular carcinoma (HCC) induced by purified afla-toxin B1 (AFB1)were as follows. (1) The precancerous lesions included hepatocytic dysplasia (58.6%), eosinocytic proliferative foci (51.61%) with most positive results by indirect immunoperoxidase-labelling technique for ?-fetoprotein (IIP-AFP), and basophilic proliferative foci with all negative results by IIP-AFP. (2) In 11 cases of HCC, there were 7 with multicentric origin and 4 with single origin, and 9 of 11 cases were in gradeⅠ differentiation and 2 in grade Ⅱ differentiation. (3) In 11 cases of HCC, 7 were of large cell type of HCC and 4 small cell type of HCC. (4) The specimens of 6 cases of HCC were examined by electronic microscopy, and it was found that the differentiation in the large cell type of HCC was better than that in the small cell type of HCC. The authors assume that there may exist multicentric and single origins in the histogenesis of HCC, and AFB1 is likely to be a carcinogen of human non-cirrhotic HCC.
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pathological observations on 32 surgical cases of noncirrhotic hepatocellular carcin-oma(NCH)were made. 12 surgical cases of cirrhotic hepatocellular carcinoma(CH) were taken as control. Routine fixed method were made by immunoperoxidase staining for AFP.The results were as follows. (1)The positive rate of NCH group host liver is somewhat higher than those of cancer tissue.AFP positive meterials were also observated in 3 cases with proliferative bile-duct epithelium, as compared with CH group,the AFP positive materials distributed in the tissue are similar to those in she cells.The differences of the AFP intensity and the positive rate of NCH group host liver are less than that of CH group.(2)In NCH group, the rate of positive cases of hepatis B is 53.13 %.it is markedly higher than that in 28.13% of orcein negative hepatitiis. (3)The AFP distributing in the cancer tissue and cancer cells were similar to those in the host liver tissue.but much more distributing in the cancer peri-trabecula.(4)It suggested that the review study by routine fixztion and staining immunoperxidase method for AFP is available progressed.