Simple nucleus pulposus removal for the treatment of prolapsed and displaced lumbar disc herniation / 中国骨伤

OBJECTIVE@#To explore the clinical effect of the simple nucleus pulposus removal and small incision interlaminar window in the treatment of prolapsed and displaced lumbar disc herniation.@*METHODS@#From February 2016 to February 2018, 35 patients with single-segment prolapse and displaced lumbar disc herniation were treated by the simple nucleus pulposus removal and small incision interlaminar window under general anesthesia. Among them, there were 21 males and 14 females;aged (42±17) years;27 cases of L@*RESULTS@#All the operations were successful and the operation time was 30 to 60 min with an average of 40 min, the intraoperative blood loss was 10 to 30 ml with an average of 20 ml. All the patients were followed up for 1 to 3 years with an average of 1.2 years. Thirty-five patients with low back pain and lower limb symptoms were significantly relieved or disappeared. According to modified Macnab standard, 29 cases obtained excellent results, 5 good, and 1 fair.@*CONCLUSION@#Applying the concept of minimally invasive operation, small incision interlaminar window and simple nucleus pulposus removal for the treatment of prolapsed and displaced lumbar disc herniation has the advantages of short operation time, definite curative effect, and less trauma. And it is a safe and effective surgical method under the premise of strict control of the indications.

Perioperative intensive statin therapy improves outcomes in patients with ischemic stroke undergoing middle cerebral artery stent implantation / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate whether intensive statin therapy during the perioperative period improves outcomes in patients undergoing middle cerebral artery (MCA) stent implantation for ischemic stroke.</p><p><b>METHODS</b>Forty patients with ischemic stroke undergoing delayed stent implantation in our department from January, 2010 to November, 2014 were randomized to intensive statin group (atorvastatin, 80 mg/day, 3 days before till 3 days after intervention; n=20) and standard therapy group (atorvastatin, 20 mg/day, n=20). All the patients received long-term atorvastatin treatment thereafter (20 mg/day). Serum levels of C-reactive protein (CRP), vascular cell adhesion molecule-1 (VCAM-1), and soluble extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) were measured at 24 h before and 24 h after the intervention. The primary end point was procedure-related intra-stent thrombosis, 1-month incidence of major adverse cerebrovascular events (stroke, transient ischemic attack, in-stent restenosis, death or unplanned revascularization).</p><p><b>RESULTS</b>The basic clinical data were similar between the two groups before the intervention (P>0.05). In the intensive therapy group, the levels of CRP, VCAM-1, and sCD147 were significantly lower at 24 h after the intervention than the levels before intervention (P<0.05) and the postoperative levels in the standard therapy group (P<0.05). The levels of CRP, VCAM-1, and sCD147 were all increased after the intervention in the standard therapy group (P>0.05). The incidence of primary end point was lower in intensive therapy group than in standard therapy group (P<0.05).</p><p><b>CONCLUSION</b>In patients undergoing MCA intravascular stent implantation for ischemic stroke, perioperative intensive statin therapy improves the patients' outcomes, reduces the levels of CRP, VCAM-1 and sCD147 molecules, and lowers the incidences of cerebrovascular events.</p>

Effect of total saponins from Sanguisorba officinalis on megakaryocyte progenitor cells proliferation, differentiation and relative receptor expression / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the effects of total saponins from Sanguisorba officinalis (DYS) on hematopoietic cell proliferation, differentiation and the expression level of IL-3R and c-kit.</p><p><b>METHOD</b>Baf3 and 32D cells were cultured with or without IL-3, then the cells were exposed to DYS in different concentrations of 5, 10, 20, 30 and 40 mg x L(-1) for 24, 48, 72 and 96 hours separately. After that, the cell proliferation and differentiation capacity were determinated by the methods of CCK8 and Giemsa staining separately. The effects of DYS on the expression level of IL-3 receptor in Baf3 cells and the expression level of c-kit in 32D cells were determinated using RT-PCR.</p><p><b>RESULT</b>DYS promotes alone proliferation of Baf3 cells and 32D cells after 48 h. In contrast to control cells, 32D cells containing DYS without IL-3 form many large clusters. DYS also increases the proliferation when cultured with IL-3. High concentration of DYS induce alone the differentiation of 32D cells and increase alone the number of the polyploidy megakaryocyte. Moreover, DYS increases alone the expression level of IL-3R in Baf3 cells and the expression level of c-kit in 32D cells separately.</p><p><b>CONCLUSION</b>Our data shows DYS can promote alone proliferation and differentiation of megakaryocyte progenitor cells. The proliferative and differentiative effect of DYS on megakaryocyte progenitor cells is correlated to the up-regulation of IL-3 receptor and c-kit expression.</p>

Efficacy of trsatuzumab (Herceptin) combined with FOLFIRI regimen in the treatment of HER2-positive advanced gastric cancer / 南方医科大学学报

<p><b>OBJECTIVE</b>To evaluate the safety and efficacy of trsatuzumab (Herceptin) combined with FOLFIRI regimen (irinotecan plus 5-FU/LV) in the treatment of HER2-positive advanced gastric cancer.</p><p><b>METHODS</b>T Thirty-four patients with pathologically confirmed advanced gastric cancer, all positive for the human epidermal growth factor receptor 2 (HER2) as identified by immunohistochemistry and fluorescence in situ hybridization, were randomized into the test group and control group to for treatment with the combined regimen and the FOLFIRI regimen alone, respectively. FOLFIRI regimen was administered every 2 weeks for 2 to 4 cycles. Trsatuzumab was given intravenously on a weekly basis with an initial dose of 4 mg/kg and a subsequent dose of 2 mg/kg. All the patients were assessed for efficacy and toxicity of the treatments.</p><p><b>RESULTS</b>The overall response rate was 58.8% in the test group and 35.3% in the control group, with disease control rates of 88.2% and 64.7%, respectively, showing significant differences between the two groups (P<0.05). The most frequent grade I/II treatment-related adverse events included diarrhea, nausea/vomiting and neutropenia.</p><p><b>CONCLUSION</b>Trsatuzumab combined with FOLFIRI regimen is effective, safe and well tolerated for treatment of HER2-positive advanced gastric cancer.</p>

Molecular characteristics and its evolution of the complete genome of avian influenza H5N1 virus isolated in Zhejiang province from 2002 to 2006 / 中华流行病学杂志

Objective To analyze the molecular characteristics and evolution reassortment of the complete genome of avian influenza H5N1 virus isolated in Zhejiang province in recent years. Methods Complete genomes of avian influenza H5N1 viruses isolated in Zhejiang province from 2002 to 2006 were sequenced. Molecular and evolution reassortment characterization of these virus strains were analyzed by Mega 3.0 bioinformatics software. Results Through study on the HA genes from all these strains,our data revealed that there were multiple basic amino acids at the cleavage site, which was typical for HPAIV.Compared with Gs/Guangdong/1/96,all these strains had a 20 amino acid deletion in the stalk of the NA, except for Dk/Zhejiang/2/02 and Ck/Zhejiang/8/03. Results from phylogenetic analysis showed that from2002 to 2003 the H5N1 viruses belonged to the genotype of B,W,X,Y,Z, and other genotypes were prevailed in corresponding year. However, different gene fragments of several strains belonged to different genotypes. Conclusion Recombinant might be widespread among the poultry in Zhejiang province. The genetic genes of viruses isolated after 2005, the Ck/Zhejiang/24/05 and Zhejiang/16/06 strains were almost all originated from the FJ-like genotype stably.

Synthesis of phenyloxyisobutyric acid derivatives and their antidiabetic activity in vitro / 药学学报

<p><b>AIM</b>To design and synthesize new phenyloxyisobutyric acid analogues as antidiabetic compounds.</p><p><b>METHODS</b>Eight new target compounds were synthesized by combination of lipophilic moieties and acidic moiety with nucleophilic replacement or Mitsunobu condensation. The eight compounds were confirmed by 1H NMR, 13C NMR, IR and MS.</p><p><b>RESULTS</b>In vitro insulin-sensitizing activity (3T3-L1 adipocyte) demonstrated, that the cultured glucose concentration of up-clear solution detected with GOD-POD assay were 5.942, 6.339, 6.226 and 6.512 mmol x L(-1), respectively, when rosiglitazone, pioglitazone, compounds A and B were added to the insulin-resistant system.</p><p><b>CONCLUSION</b>In vitro insulin-sensitizing activity of target compound A is in between that of rosiglitazone and pioglitazone, and activity of target compound B is slightly less than that of pioglitazone.</p>

Design, synthesis and insulin-sensitizing activity of some peroxisome proliferator-activated gamma agonists / 药学学报

<p><b>AIM</b>To find new peroxisome proliferator-activated y agonists with high activity and low toxicity.</p><p><b>METHODS</b>Based on JTT-501 and JTT-20993, new isoxazolidine-3,5-dione and noncyclic 1,3-dicarbonyl compounds were designed and synthesized. Their insulin-sensitizing activities were evaluated.</p><p><b>RESULTS</b>Eight new compounds were obtained. The structures of synthesized compounds were characterized by NMR, MS and IR. Four compounds (1A-4A) showed insulin-sensitizing activities.</p><p><b>CONCLUSION</b>Compounds (1A and 3A) showed excellent insulin-sensitizing activities and should be worth further investigation.</p>

Influence of Pueraria thomsonii on insulin resistance induced by dexamethasone / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the influence of Pueraria thomsonii on insulin resistance induced by dexamethasone.</p><p><b>METHOD</b>3T3-L1 adipocytes model of insulin resistance was made by dexamethasone and the change of glucose concentration in cell culture was determined after action of drugs. Rat animal model of insulin resistance was made by intramuscular dexamethasone (1 mg x kg(-1), every other day), and fasting blood glucose (FBG) and fasting serum insulin (FINS) were enamined, and at the end of the experiment, insulin sensitive index (ISI) and insulin resistance index (IRI) were calculated.</p><p><b>RESULT</b>P. thomsonii decreased the concentration of glucose in 3T3-L1 adipocytes culture significantly and improved the sensitivity of 3T3-L1 adipocytes to insulin. P. thomsonii improved the sensitivity of rats to insulin and diminished the fasting serum insulin and insulin resistance index.</p><p><b>CONCLUSION</b>P. thomsonii can significantly improve insulin resistance induced by dexamethasone.</p>

Screening of angiotensin converting enzyme inhibitors from Salvia miltiorrhizae / 中国中药杂志

<p><b>OBJECTIVE</b>To screen angiotensin converting enzyme inhibitor (ACEI) from traditional Chinese medicine, Salvia miltiorrhiza.</p><p><b>METHOD</b>Hydrophilic and lipophilic fractions of S. miltiorrhiza were isolated, and their effective components were screened by a fluorimetric assay for inhibition of angiotensin converting enzyme (ACE).</p><p><b>RESULT</b>Water-soluble fractions, total salvianolic acids and salvianolic acid B markedly decreased ACE activity of rabbit lung tissue. Their IC50 value were (2.45 +/- 0.07), (0.24 +/- 0.02), (0.02 +/- 0.01) g x L(-1) respectively. Lipophilic components or phenanthraquinones including tanshinone I and II showed no changes on the activity of ACE.</p><p><b>CONCLUSION</b>S. miltiorrhiza inhibits angiotensin converting enzyme and its active components are in aqueous extract, in which the main were salvianolic acids including salvianolic acids B.</p>