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OBJECTIVE To explore the neuroprotective effect of sodium aescinate on rats with Parkinson’s disease by regulating the silent information regulator 1 （SIRT1）/nuclear factor-κB （NF-κB） signaling pathway. METHODS The Parkinson’s disease rat model was constructed by using 6-hydroxydopamine injection method. Forty-eight rats successfully modeled were randomly divided into model group， sodium aescinate low-dose group （1.8 mg/kg）， sodium aescinate high-dose group （3.6 mg/kg）， sodium aescinate+EX527 （sodium aescinate 3.6 mg/kg+SIRT1 inhibitor EX527 5 mg/kg） group， with 12 rats in each group. Another 12 healthy rats were selected as the sham operation group. Each group was injected with the corresponding drug solution intraperitoneally， once a day， for 21 consecutive days. Twenty-four hours after the end of the last administration， the motor and cognitive functions of rats were detected， and the morphology of neurons in the substantia nigra and CA1 region of hippocampal tissue were observed. The content of dopamine （DA） in the nigrostriatal and the expression levels of tyrosine hydroxylase （TH） and α-synuclein （α-Syn） in the substantia nigra were detected. The serum levels of pro-inflammatory factor [interleukin-6 （IL-6）， IL-18]， anti-inflammatory factor （IL-10）， and the expression levels of SIRT1， phosphorylated NF-κB p65 （p-NF-κB p65） and NF- κB p65 protein in nigrostriatal were detected. RESULTS Compared with sham operation group， the neurons in the substantia nigra and CA1 region of hippocampal tissue were seriously damaged in model group； the number of rotations， escape latency， the expression levels of α-Syn in substantia nigra， the levels of serum pro-inflammatory factors， the relative expression ratio of p-NF- κB p65 and NF-κB p65 protein in nigrostriatal were increased or prolonged significantly （P＜0.05）； the target quadrant residence time， the content of DA in nigrostriatal， the expression level of TH in substantia nigra， the serum level of anti-inflammatory factor， and the expression level of SIRT1 protein in substantia nigra striatum were significantly decreased or shortened （P＜0.05）. Compared with model group， the damage degrees of neuron in sodium aescinate groups were alleviated， and the quantitative indicators were significantly improved， which were more significant in the high-dose group （P＜0.05）； EX527 could reverse the improvement effect of high-dose sodium aescinate （P＜0.05）. CONCLUSIONS Sodium aescinate can inhibit the activation of NF-κB signal by up-regulating the protein expression of SIRT1， thereby reducing the neuroinflammation of rats with Parkinson’s disease， improving the motor and cognitive dysfunctions， and finally playing a neuroprotective role.
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Aim To study the inhibitory effects of sodium aescinate in liver fibrosis induced by carbon tetrachloride (CCl4 ). Methods Forty-one male SD rats were recruited in this study and randomized into control group (n = 5), model group (n = 18), and sodium aescinate treated group (n = 18) . Masson staining was performed for collagen fiber detecting, and IHC staining was conducted for accessing the expression of interest proteins in rat liver. MTT and apoptosis assay were performed to evaluate the effects of sodium aescinate on HSC-T6 cells. The expression of interest proteins was detected by immunoblotting. Results Sodium aescinate alleviated the liver fibrosis induced by CCl4 through proliferation inhibition and apoptosis induction in HSC-T6 cells. Sodium aescinate also down-regulated the phosphorylation of 4EBP1, the expression of collagen I and collagen III. Conclusion Sodium aescinate alleviates liver fibrosis induced by CCl4,.
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Objective To establish a fast detection method of sodium aescinate by using near infrared (NIR) spectroscopy analysis method for the determination of the content of sodium aescinate for injection. Methods OPUS software was used to optimize the collected spectrum. PLS algorithm and factorization algorithm were used to establish quantitative model and qualitative model. Results The correlation coefficient of the quantitative model reached 0.9926, the RMSECV deviation was 0.253. The deviation between the predicted value of the sample and the true measured value was less than 5%, which could accurately predict the content of sodium aescinate. Conclution The qualitative model can effectively distinguish the samples of other varieties that have not participated in the modeling, and provide a reference for the rapid screening of the drug.
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Aim To investigate the correlation between the expression of EIF4A1 and hepatocellular carcinoma (HCC) and the potential mechanism of sodium aescinate inhibiting the proliferation of hepatoma cell lines. Methods Immunohistochemistry was used to detect the expression level of EIF4A1 in tumor specimens of 80 patients with HCC. The results combined with clinical indicators and follow-up information were used to analyze their relevance. Annexin V-FITC/PI double staining method was employed to detect the effects of sodium aescinate on apoptosis of HepG2 and human L02 cell lines. Transwell migration assay was used to detect the effect of sodium aescinate on the migration of two cell lines. Western blot, qPCR and immunofluorescence were used to detect the expression changes of E1F4A1 of two cell lines after sodium aescinate treat ment. Results The expression of EIF4A1 significantly increased in HCC tissues, and the expression of EIF4A1 was correlated with tumor differentiation, tumor diameter and survival time. Sodium aescinate (40 jxmol • L"1) could significantly promote the apoptosis of liver cancer cell line and inhibit its migration a-bility, but had no effect on normal liver cell line. Sodium aescinate inhibited the growth and proliferation of hepatoma cell line while down-regulated the expression of hepatoma cell line EIF4A1. Conclusions EIF4A1 is associated with the development of HCC, and sodium aescinate can inhibit hepatoma cell line via affecting the expression of EIF4A1.
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Aim To investigate the expression of CAR-MA3, NF-κB in hepatocellular carcinoma tissues and the underlying mechanism of sodium aescinate in inhib-iting the proliferation of human hepatocellular carcino-ma cells. Methods The expression of CARMA3 and NF-κB in HCC tissues were detected by tissue microar-ray immunohistochemistry. MTT was used to determine the effect of sodium aescinate on the proliferation of HCC cells. Cell apoptosis was detected by flow cytom-etry. The expression of CARMA3, NF-κB protein in HepG2 and Hep3B cells treated with sodium aescinate was detected by Western blot and cell immunofluores-cence. Results Tissue microarray analysis showed that the expression of CARMA3 in HCC was up-regulated compared with the adjacent adjacent liver tissues, and the histopathological differentiation, TNM stage, tumor volume and prognosis were correlated. Sodium aesci-nate in 40 μmol·L-1concentration ( IC50) inhibited the growth of HCC cell lines, promoting its apoptosis, but without toxic effects on normal liver cells. Western blot and cell immunofluorescence detection of sodium aescinate could significantly inhibit the expression of CARMA3 and NF-κB. Conclusion Sodium aescinate can effectively inhibit the proliferation of HCC cells by inhibiting the activation of CARMA3/NF-κB signaling in HCC.
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Objective@#To study the effect of sodium aescinate on the development process of lung injury induced by paraquat.@*Methods@#Forty-five health adult male SD rats were randomly divided into control group, PQ group, sodium aescinate group, and 15 rats in each group. The PQ group and sodium aescinate group were given a one-time intraperitoneal injection of 18mg/kg body weight of rats PQ, the control group was given the same amout normal saline. Rats in sodium aescinate group were injected 2 mg/kg body weight sodium aescinate into abdominal cavity for 7 days continually, but the same volume of saline was injected into the other groups. Finally, at 7, 14 and 28 days after PQ poisoning, five rats were kills for measuring lung tissue pathological changes and the value of TGF-β1, TNF-α, hydroxyproline in each group.@*Results@#The expression of TNF-α in serum of 7th day [ (17.03±0.82) ng/ml] and 14th day [ (15.74±0.91) ng/ml] of sodium aescinate group were lower than the corresponding period of PQ groups’, and the difference had statistical significance (P<0.05) . The expression of TGF-β1 in serum of 7th day[ (225.93±8.33) ng/ml], 14th day [ (216.62±9.48) ng/ml] and 28th[ (181.41±6.10) ng/ml] of sodium aescinate group were lower than the corresponding period of PQ groups’, and the difference had statistical significance (P<0.05) . Lung tissue pathological changes showed, compared with control group, inflammatory injury at 7th day and fibrosis degree at 28th of rats’ lung reduced on sodium aescinate group. The expression of hydroxyproline in rats’ lung of 7th day[ (1.246±0.018) μg/mg], 14th day [ (1.269±0.034) μg/mg] and 28th[ (1.283±0.028) μg/mg] of sodium aescinate group were lower than the corresponding period of PQ groups’, and the difference had statistical significance (P<0.05) .@*Conclusion@#sodium aescinate could reduce the pulmonary inflammatory injury and hydroxyproline value of PQ poisoning rats, so sodium aescinate could ameliorate lung injury induced by PQ.
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Objective To explore sodium aescinate drug extravasation treatment method with method of evidence-based medicine. Methods According to the clinical symptoms of a patient who suffered the extravasation of sodium aescinate, electronic databases were searched to collect relevant materials of preventing extravasation of sodium aescinate. The evidence was applied to the patient after evaluating the reality, feasibility of these evidences mentioned in the literatures. Results Eventually, six random trials, five clinical experience, and two literature reviews were included. The collected evidences supported that sanyrene was safe and effective in treating the extravasation of sodium aescinate. The color and function the patient′s hand returned to normal after using sanyrene in three days, meanwhile, irreversible complication was avoided. Conclusions Sanyrene applied to sodium aescinate extravasation,easy to operate,the effect is obvious,it is worth promoting.
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Objective To study the effect of Shugan Tongluo Recipe on the patients with upper limb swelling after breast cancer surgery, and to explore the efficiency of treatment.Methods84 patients with breast cancer were selected from June to December 2015 in the traditional Chinese medicine clinic of Ge zhen,, were randomly divided into control group and observation group, control group using beta seven yezao sodium and conventional rehabilitation therapy, observation group based on the combined application of Shugan Tongluo Decoction, and compared the effectiveness of treatment.ResultsThe effective rate of the observation group was 92.8%, significantly better than the control group 78.6%, the difference between the two groups significantly, with statistical significance (P<0.05).At the same time, the observation group VAS score, IL-6 score and CCL-18 score were lower than the control group, EORTC QLQ score is higher than that of the control group, the treatment of feedback better(P<0.05).In addition, the incidence of adverse reactions was 4.7%, 9.5% in the control group, the observation group treatment.ConclusionThe prescription of Shugan Tongluo decoction has a good effect on the treatment of upper limb swelling after breast cancer surgery, and it has high safety and good patient satisfaction.
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Objective To study the effect ofβ?sodium aescinate on the expressions of aquaporin?4 and aquaporin?9 in rats with spinal cord inju?ry. Methods A total of 150 rats were randomly divided into 3 groups:sham group(n=50),spinal cord injury(SCI)group(n=50)andβ?sodi?um aescinate group(n=50). The experimental animal models was established by modified Allen’s model. The Basso,Beattie and Bresnahan (BBB)locomotor rating scale and inclined plane test were used to evaluate rat behavioral consequences after injury.The immunohistochemical staining and western blotting assay were performed to observe the expressions of aquaporin?4 and aquaporin?9. Results Compared with sham group,BBB score and inclined plane test score of SCI group andβ?sodium aescinate group were significantly lower at each time point(P<0.05);however,the functional recovery was significantly better inβ?sodium aescinate group than in SCI group at each time point from 7 d after SCI(P<0.05). The aquaporin?4 and aquaporin?9 positive expressions of rats in sham group were lower significantly than rats in SCI group andβ?sodium aescinate group(P<0.05);however,the aquaporin 4 and aquaporin 9 positive expressions of rats inβ?sodium aescinate group was lower signifi?cantly than rats in SCI group at each time point(P<0.05). Conclusion β?sodium aescinate can protect the neurologic function in rats with spi?nal cord injury by decreasing aquaporin?4 and aquaporin?9 protein expression.
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Sodium aescinate, which is produced from saponins of Chinese Buckeye Seed, is a prescription drug for treatment of brain edema and all kinds of swellings caused by surgery. In this article, high-performance liquid chromatography/ion trap (HPLC-IT) mass spectrometry was applied to study the characteristic ions of ten reference substances, namely escin Ⅰa, escin Ⅰb, isoescin Ⅰa, isoescin Ⅰb, aesculiside A, aesculiside B, aesculuside A, escin Ⅳc, escinⅡa and escin Ⅴ, which were isolated from aescinate. Furthermore, 19 saponin compounds were predicted in sodium aescinate, besides the above mentioned reference substances. The study showed that sapogenins in sodium aescinate had two structural types, namely protoaescigenin and barringenol C, and the substituent acetyl, tigloyl or angeloyl was usually located at C-21, C-22 or C-28 position. Among these predicted saponins, their sugar chains were all located at C-3 position consisting of glucose and glucuronide. This study provides experimental data for chemical constituents in sodium aescinate and scientific basis for quality and safety evaluation.
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OBJECTIVE:To provide reference for clinical safe administration and drug instruction revision through analyzing 552 cases of ADR/ADE induced by Sodium aescinate for injection. METHODS:Using“aescinate”and“ADR”as search words,re-trieved from CBM,CNKI,VIP and Wanfang database,47 literatures were included according to inclusion and exclusion criteria. The data was extracted and analyzed statistically. The drug instructions of twelve domestic pharmaceutical enterprises were analyzed statistically. RESULTS:The ADR/ADEs may involve multiple organs and systems,the most common reactions were venous irritant reactions as phlebitis,venous injury,etc. The drug instruction lacked some parts of information. CONCLUSIONS:Great impor-tance should be attaches to ADR/ADEs caused by Sodium aescinate for injection,and improvement of the drug instruction to en-sure the safety of drug use in the clinic.
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Objective To explore β-sodium aescinate on vascular endothelial function ( FMD ) , homocysteine ( Hcy ) and hypersensitive C-reactive protein ( hs-CRP) and clinical efficacy in patients with acute cerebral infarction.Methods 198 acute cerebral infarction patients from March 2013 to April 2015 were randomly divided into observation group (n=100) and control group (n=98).Control group were treated according to the condition of the disease, observation group were treated by β-sodium aescinate base on control group, 20mg was added to 250mL saline for intravenous drip,one times per day.Continuous used 14d for one treatment courses.Compared the change of vascular endothelial function, Hcy and hs-CRP and clinical efficacy.Results The total effective rate of observation group was 90.00%, which was significantly higher than that of 71.42% in control group (χ2 =11.01,P<0.05).Post-treatment the value of FMD significantly increased, Hcy and hs CRP were significantly decreased both in observation group and control group respectively, which the difference had a statistically significant as compared with Pre-treatment (P<0.05);but, the value of FMD was significantly higher, Hcy and hs CRP was significantly lower in observation group than that of control group (P<0.05).Conclusion It has a significant β-sodium aescinate clinical effect in treatment of acute cerebral infarction, and FMD are significantly higher, Hcy and hs-CRP are significantly decrease.
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Objective To investigate the therapeutic effect of sodium aescinate (SA) on severe acute pancreatitis (SAP) in rats.Methods SAP model was induced by intraductal administration of 5％ taurocholic acid sodium salt.SD rats were then randomly divided into 3 groups (n =16),namely sham,model and SA group (5 mg/kg).Drugs or saline(10 ml/kg) were administered via caudal vein 30 min post-operation.The serum amylase was measured with spectrophotometer and pancreatic histological changes were observed under microscope 6 h and 12 h post-operation,respectiveiy.Results Pancreatic histological grades in the model group were (9.42 ± 1.06) and (18.30± 2.18) 6 h and 12 h post-operation,respectively,which showed significant differences (P＜0.01) compared with the sham group [(0.55 ±0.20) and 0,respectively].SA evidently reduced the severity of pancreatic pathology and improved the tissue inflammation in rats with SAP.Pancreatic histological grades were (7.85±1.33) and (12.75±1.69)in the SA group 6 h and 12 h post-operation,respectively,which had significant differences compared with the model group (P＜0.05).Serum amylase levels in model group were (2612 ± 59) U/L and(3004±687)U/L6 h and 12 h post-operation,respectively,which showed significant differences (P ＜ 0.01) compared with the sham group [(928 ± 271) and (890± 295) U/L,respectively].Serum amylase levels were reduced to (1790± 336) U/L and (2093 ± 298) U/L in the SA group 6 h and 12 h post-operation,respectively.SA markedly inhibited the level of serum amylase compared to the model group (P＜0.01).Conclusion SA could protect rats with SAP effectively.
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Objective:To investigate the effects of sodium aescinate on endothelin ( ET ) and calcitonin gene -related peptide ( CGRP) contents in the gerbils with cerebral ischemia/reperfusion injury ( CI/R) . Methods:The gerbil model of CI/R was prepared by bilateral common carotid arteries ligation for 10 min followed by 2-hour reperfusion. Sodium aescinate (i. p. , 10, 20 and 40 mg· kg-1 ) was administered once a day for 3 days before the operation and once every 1 h after the operation, respectively. The levels of ET and CGRP in brain tissue homogenate were determined by a radioimmunoassay method. Results:Sodium aescinate significantly in-hibited the level of ET (28. 69-37. 03 ng·L-1) in the brain tissue of gerbils with CI/R compared with the model group (P0. 05). Conclusion:Sodium aescinate has obvious protective effects a-gainst CI/R in gerbils, which may be due to its inhibitory action on ET levels in brain tissue.
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Objective To investigate the clinical effects of sodium aescinate adjuvant in treatment with tibial plateau fracture. Method According to the principle of random draw, 160 cases of tibial plateau fractures patients were divided into observation group and control group, each had 80 cases. All cases were given the improved bilateral plate internal ifxation with conventional interventions, besides, the observation group were added intravenous infusion of sodium aescinate after surgery. Results The differences of operative time, blood loss and clinical fracture healing time between two groups were not signiifcant. After intervention, the overall incidence of complications with arthritis, deep vein thrombosis, joint stiffness and joint instability in observation group were signiifcantly lower than that in control group, but the value of joint activity with the HSS values were signiifcantly higher(P<0.05). The plasma TNF-αlevels between two groups before the intervention was not signiifcant, and the values were signiifcantly decreased after the intervention (P<0.05), and had signiifcant differences between the two groups (P<0.05). Conclusion Sodium aescinate has good effect on prognosis in treatment with tibial plateau fractures, the mechanism may be related to reducing the level of plasma TNF-α.
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BACKGROUND: This study was undertaken to investigate the expression of hypoxia-inducible factor-1α (HIF-1α) in rat cerebral cortex and the effects of β-sodium aescinate (SA) administration after return of spontaneous circulation (ROSC).METHODS: Sixty rats were divided into three groups: SA group, injected intraperitoneally with SA instantly after ROSC; control group, injected intraperitoneally with normal saline; and sham-operated group, without cardiac arrest or SA. The cardiac arrest model was established using asphyxiation and intravenous potassium chloride. Blood was sampled 1, 6, 12, and 24 hours after ROSC. Protein and mRNA levels of HIF-1α, VEGF and EPO were detected in the cerebral cortex by immunohistochemistry and real-time RT-PCR; serum levels of NSE and S100β were determined by enzyme-linked immunosorbent assays.RESULTS: Serum S100β and NSE were signifi cantly increased in the control group versus the sham-operated group 1, 6, 12 and 24 hours after ROSC (P<0.05). Protein and mRNA levels of HIF-1α, VEGF and EPO were signifi cantly increased in the control rats (P<0.05). Serum NSE and S100β were significantly decreased in the SA group versus the control group 1, 6, 12 and 24 hours after ROSC (P<0.05). Protein and mRNA levels of HIF-1α, VEGF and EPO were signifi cantly increased in the SA group (P<0.05).CONCLUSIONS: The expression of HIF-1α is increased in rat cerebral cortex after ROSC, and SA up-regulates the expression of HIF-1α. The up-regulation of HIF-1α improves the resistance of the cortex to ischemia and hypoxia and contributes to neuroprotection, possibly because of up-regulation of EPO and VEGF expression.
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Objective To investigate the expression of the hypoxia-inducible factor (HIF)-1α in rat brain neurons and the intervention of β-sodium aescinate after restoration of spontaneous circulation (ROSC).Methods Sixty SD adult rats were randomly (random number) divided into 3 groups (n =20),namely experiment group,control group and sham operation group.(1) The rats of experiment group were injected intraperitoneally with β-sodium aescinate (5 mg/kg) immediately after ROSC.(2) The rats of control group received normal saline injected intraperitoneally instead of β-sodium aescinate solution.(3)The rats of sham operation group did not have cardiac arrest and β-sodium aescinate intervention.Cardiac arrest rat model was established by using asphyxiation and intra-venous potassium chloride solution.Blood samples were taken 1 h,6 h,12 h and 24 h after ROSC,and subsequently rats were sacrificed and their brain tissues were harvested.The expressions of HIF-1 α mRNA,vascular endothelial growth factor (VEGF)mRNA and erythropoitin (EPO) mRNA and their protein levels in rat brain neurons were detected by using RT-PCR and immunohistochemistry,and the levels of serum neuron-specific enolase (NSE) and S100β proteins were determined by using enzyme-linked immunosorbent assay.The t test or one-way ANOVA was used to assess overall differences among groups for each of the variables,followed by Bonferroni test for multiple comparisons.Pearson method was used for correlation analysis.Results Compared with the sham operation group at intervals of 1 h,6 h,12 h and 24 h after ROSC,levels of serum S100β and NSE proteins were significantly increased in rats of the control group (P ＜ 0.05).Meanwhile,the expressions of HIF-1 α mRNA,VEGF mRNA and EPO mRNA and their protein levels in rat brain neurons were significantly increased in the control rats (P ＜0.05).Compared with the control group at intervals of 1 h,6 h,12 h and 24 h after ROSC,levels of serum NSE and S100β proteins were significantly decreased in rats of the experiment group (P ＜ 0.05).Whereas,the expressions of HIF-1 α mRNA,VEGF mRNA and EPO mRNA and their protein levels in rat brain neurons were significantly increased in rats of the experiment group (P ＜0.05).HIF-1 α mRNA was positively correlated with EPO mRNA and VEGF mRNAs (r =O.866,P ＜0.05 ; r =0.952,P ＜ O.01).Conclusions The expression of hypoxia-inducible factor-1 α is increased in rat brain cells after ROSC,and β-sodium aescinate up-regulates the expression of hypoxia-inducible factor1 α mRNA and protein levels.The up-regulated expression of hypoxia-inducible factor-1α improves the resistance of brain cells to ischemia and hypoxia contributing neuronal protection,which might be due to upregulated EPO and VEGF expressions induced by hypoxia-inducible factor-1α.
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Objective To study the anti-leakage mechanism and protective effect of sodium aescinate on the blood-brain barrier of rats acutely exposed to hypoxia. Methods Seventy-five healthy SD rats were randomly divided into 3 groups (25 each): normoxic control (NC), simple hypoxic (SH) and drug treated (DT) group. Acute hypoxia brain edema rat model was established by a simulation of acute high-altitude hypoxia for 5 days. The cerebral water content was determined by dry-wet method. The permeability of the blood-brain barrier (BBB) was evaluated by Evans blue (EB) method. The pathological change of the brain was detected by HE staining. The state of BBB tight junction (TJ) and ultrastructures of the brain tissues were observed by lanthanum nitrate tracer method under transmission electron microscope (TEM). Protein and mRNA expression of Occludin, Zo-1 and Claudin-5 were investigated by immunohistochemistry, Western-blotting and real-time PCR respectively. Results After exposure to acute hypoxia for 5 days, compared with NC group, the water content of brain in SH group increased obviously (P<0.01) while that in DT group decreased significantly (P<0.05), and EB content analysis revealed a similar result. It was observed by microscopy that hippocampus neurons were lost and edemas occurred heavily in SH group while slightly in DT group. It was detected by TEM that lanthanum nitrate infiltrated and deposited into cerebral interstitium through widened TJ gap of the brain microvascular endothelial cells (BMVECs), and the perivascular edema was obvious in SH group, while these changes were lessened in DT group. The lowest expression levels of Occludin mRNA and protein were found in SH group (P<0.01), and the highest levels in DT group (P<0.05). Meanwhile, to compare with SH group, the expression of Zo-1 and Claudin-5 mRNA increased remarkably in both NC and DT group (P<0.05), while no significant difference existed between the latter two groups. Conclusion Acute hypoxia exposure may lead to a remarkable decline of the expressions of rat's brain Occludin protein and the Occludin, Zo-1 and Claudin-5 mRNA, and an obvious increase of BBB permeability. Sodium aescinate can up-regulate the expression level of these molecules and decrease BBB permeability, thus playing a profitable role of anti-leakage and BBB protection.
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ObjectiveTo observe the clinical effect of sodium aescinate in the treatment of patients with stage Ⅰ - Ⅱ internal hemorrhoids.Methods177 patients with stage Ⅰ - Ⅱ internal hemorrhoids were randomly divided into two groups,the treated group( n =87 ) and the control group( n =90).Patients in the control group were treated with external hemorrhoids suppositories ( 1 per time,bid),while patients in the treated group took sodium aescinate orally(2 per time,Bid).After the 7-day course of treatment,the efficacy of sodium aescinate was determined.ResultsThere was significant difference between the two groups in the total obvious effective rate and the total effective rate(P ＜0.01 ).Compared with the control group,the symptoms of hematochezia and anal pain were more improved in the treated group( P ＜ 0.01 ).There was significant difference between the two groups in the hemorrhoidal mucosa,prolapse,the size of hemorrhoids and total score difference ( P ＜ 0.05 ).The total obvious effective rate and the total effective rate of the treated group were 87.4％ and 95.4％.ConclusionSodium aescinate had better clinical effect in the treatment of patients with stage Ⅰ - Ⅱ internal hemorrhoids.
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Objective To investigate the effects of sodium aescinate(SA)on oxidative stress and pulmonary function during acute exacerbation of chronic obstructive pulmonary disease(COPD).Methods One hundred and twenty patients with COPD were randomly divided into two groups:the control group(n =60) and the treatment group(n =60).All patients were treated with routine anti-infection,oxygen inhalation,relieving phlegm and anti-asthma The treatment group took SA in addition to the routine beteropathy.The changes of serum SOD,MDA,GSH-Px,T-AOC,pulmonary functions and 6 minute walk distance(6MWD) were detected before and after two-week treatment in patients of the two groups to compare with 60 healthy subjects.Results The total effective rate in the treatment group was 91.67％,while 76.67％ in the control group.The difference was statistically significant(x2 =5.065,P ＜0.05).Serum MDA level in both groups were comparatively higher than the healthy controls(9.25±1.55) μmol/L vs.(9.74±1.50) μmol/L vs.(2.06±0.29) μmol/L,P ＜0.001),while the levels of SOD,GSH-Px and T-AOC were lower than the healthy controls[SOD:(91.14±9.54) kU/L vs.(90.61±8.01) kU/L vs.(116.63±6.57) kU/L; GSH-Px:(139.38±36.56) U vs.(137.57±34.19) U/L vs.(189.34±35.54) U/L; T-AOC:(6.48±1.15) kU/L vs.(6.39±1.13) kU/L vs.(13.34±1.23)kU/L;P < 0.001].After treatment,all indexes of the two groups were obviously ameliorated in comparison with before treatment(P ＜ 0.001),but the level of MDA[(4.56±1.39) μmol/L]in the treatment group decreased more greatly than in the control groups(P ＜ 0.001).The levels of SOD[(103.85±7.07) kU/L],GSH-Px[(169.65±34.51) U/L],T-AOC[(10.52±1.09) KU/L],forced expiratory volume in 1 second/forced vital capacity(FEV1/FVC)[(60.49±6.11)％],FEVI％[(76.62±6.35)％]and 6MWD [(394.83±10.11)m]increased considerably more than those in the control group(P ＜ 0.001).Conclusion Oxidative stress might be involved in the course of acute exacerbation of COPD.Sodium aeseinate can improve the pulmonary functions by ameliorating the oxidative stress during acute exacerbation in patients with COPD.