Comparison of methods for enriching urine proteins / 生物工程学报

The abundance of proteins in human urine is low and easily to be masked by high-abundance proteins during mass spectrometry analysis. Development of efficient and highly selective enrichment methods is therefore a prerequisite for achieving deep coverage of urine protein markers. Notably, different experimental methods would affect the urine protein enrichment efficacy and the coverage of urine proteome. In this study, ultrafiltration, nitrocellulose membrane enrichment and saturated ammonium sulfate precipitation were used to process 10 mL urine samples from five healthy volunteers and five bladder cancer patients. The urine proteins were enriched and separate by SDS-PAGE to compare the purification efficiency of different methods. Moreover, the peptide identification effects of different purification methods were analyzed by mass spectrometry to determine the best method for enriching urine protein histones. Saturated ammonium sulfate precipitation method outperformed the ultrafiltration and the nitrocellulose membrane enrichment methods in terms of the protein enrichment efficacy and quality. The interference of highly abundant albumin was reduced, whereas the amount of low-abundance protein was increased, and the sensitivity of mass spectrometry identification was increased. The saturated ammonium sulfate precipitation method may be applied for large-scale urine processing for screening clinical diagnostic markers through proteomics.

Investigation of Physicochemical Properties and Determination Methods of Liposomal Entrapment Efficiency of Dichroa Alkali Hydrochloride / 中国实验方剂学杂志

Objective: To study on the physical and chemical properties of dichroa alkali hydrochloride and to establish a method for the determination of entrapment efficiency of dichroa alkali hydrochloride liposomes. Method: HPLC was used to determine the content of dichroa alkali hydrochloride with mobile phase of acetonitrile-water-triethylamine-glacial acetic acid(9:91:0.35:0.75) and detection wavelength at 265 nm.The oil-water partition coefficient of this compound in different pH range was measured by shake flask method.The stability of the dichroa alkali hydrochloride in phosphate buffer solution with different pH after sterilization at 125℃ for 30 min was investigated.Ammonium sulfate gradient method was used to prepare dichroa alkali hydrochloride liposomes.The microcolumn was prepared by dextran gel and cation exchange resin,respectively.Then the free drug and liposome were separated by centrifugation,the drug content was measured,and the encapsulation efficiency was calculated.The t-test was performed using SPSS 20.0 software,the differences between these two methods were compared. Result: In the pH 6-9,the oil-water partition coefficient of dichroa alkali hydrochloride increased with increasing of pH,which was between 0.016 and 1.44;the recovery rate of dichroa alkali hydrochloride after sterilization was 37.16%-57.91%.Between the dextran gel microcolumn centrifugation and the cation exchange resin microcolumn centrifugation,there was no significant difference in the entrapment efficiency of the liposomes. Conclusion: Dichroa alkali hydrochloride is suitable for preparation of liposomes.However,its stability is not ideal,so the experimental temperature should be strictly controlled in the preparation process.Dextran gel microcolumn centrifugation and cation exchange resin microcolumn centrifugation can be used to determine the entrapment efficiency of dichroa alkali hydrochloride liposomes,and the cation exchange resin microcolumn centrifugation is suggested after comparison.

Formulation and Efficacy of Liposome-encapsulated Afatinib for Therapy of Non-small Cell Lung Cancer / 中国肺癌杂志

BACKGROUND@#Afatinib, a second-generation irreversible epidermal growth factor inhibitor receptor for the development of non-small cell lung cancer and secondary drug resistance, has low bioavailability and adverse reactions due to current oral administration. The aim of this study was to prepare a novel drug delivery system, afatinib liposome, and to establish a method for the determination of encapsulation efficiency.@*METHODS@#Four different preparation methods were used to prepare afatinib liposomes, and the optimal preparation process was determined by comparing the encapsulation efficiency and particle size.@*RESULTS@#It has been verified that sephadex microcolumn centrifugation can be used to purify afatinib liposomes, and UV spectrophotometry can be employed to determine the entrapment efficiency of liposomes. Among different preparation methods, the encapsulation efficiency of afatinib liposomes prepared by ammonium sulfate gradient method was 90.73% and the average particle size was 108.6 nm.@*CONCLUSIONS@#Ammonium sulfate gradient method can be successfully applied to prepare afatinib liposomes that performed higher encapsulation efficiency and smaller particle size. The UV spectrophotometry employed to determine the liposome encapsulation efficiency was easy operation and with high accuracy.

Preparation of human transferrin and verification of the antigenic activity / 国际检验医学杂志

Objective Non-affinity methods were used to purify transferrin(TRF)with high purity from human serum,and the immunogenicity of TRF was evaluated by immunizing New Zealand rabbits.Methods Firstly,TRF was extracted from serum by precipitation with ammonium sulfate and then purified by two-step anion exchange chromatography.Results SDS-PAGE purity of the prepared TRF was similar with the control pure product,and the HPLC purity reached to 96.8% with a yield of 78.6%.For the same batch of TRF sam-ple,the ratio between the activity concentration determinated using TRF kit(immunoturbidimetry method) and the protein concentration determinated using uv-spectrophotometric method was about 0.95,which indica-ted that the prepared TRF for antigen could react well with the TRF antibody included in the TRF kit.Final-ly,New Zealand rabbits were immunized using the purified TRF,and the titer of the rabbit anti-serum could reach 1:128 000 after four time immunization,which also indicated that the prepared TRF had good immuno-genicity.Conclusion The TRF with high purity had good antigen reactivity and immunogenicity to prepare anti-T RF antibody by immunizing rabbits,w hich could provide qualified materials for the production of T RF kit(immunoturbidimetry method).

Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM / Avaliação da produção de enzimas holocelulolíticas por Lentinula edodes (Berk.) Pegler durante o crescimento em fermentação submersa usando MSR

The cellulase proteins have a great importance in the enzymatic hydrolysis of woody biomass. Despite of costs being a major concern, it has been a stimulus to study basidiomycetes biochemical properties which degrade lignocellulosic material and have prompted the processes' study for obtaining cellulolytic enzymes in fungi. The objective of this research was to evaluate the effects of the initial nitrogen content on (ammonium sulfate) and on sugar cane bagasse, which hereby, acts as an inducer of hydrolytic enzymes to produce cellulases and xylanases, using three Lentinula edodes (Berk.) Pegler strains as a transformation agent. A factorial design with 22 replications in the central point was conducted, varying concentrations of ammonium sulfate and sugar cane bagasse. The submerged cultures carried out in synthetic culture medium and incubated at 25°C for 7 days on an orbital shaker at 150 rpm. The total protein and cellulase activity as endoglucanase, exoglucanase and β-glucosidase and the xylanase was also determined. The results showed that the production of hydrolytic enzymes was stimulated by the presence of high concentrations of sugar cane bagasse (30g/L), characterizing it as an inducer due to the demonstrated proportional relationship. Thus, ammonium sulfate acted as a reducing agent in the synthesis of enzymes, being the low concentrations (0.1g/L) indicated for the enzyme production system under study. Among the studied strains, the EF52 showed higher activity for xylanase, endoglucanases, β-glucosidase and also protein.

As celulases são proteínas de grande importância na hidrólise enzimática de biomassa florestal. No entanto, seu custo elevado tem estimulado o estudo de processos de obtenção de enzimas celulolíticas por fungos filamentosos, tais como os basidiomicetos que apresentam propriedades bioquímicas para degradação de material lignocelulósico. O objetivo deste trabalho foi avaliar os efeitos do teor inicial de nitrogênio (sulfato de amônia) e de um indutor de enzimas hidrolíticas (bagaço de cana de açúcar) na produção de xilanases e celulases utilizando três isolados de Lentinula edodes (Berk.) Pegler como agente de transformação. Foi realizado um planejamento fatorial 22 com repetição no ponto central, variando as concentrações de sulfato de amônia e bagaço de cana de açúcar. O cultivo submerso realizado em meio de cultivo sintético e incubado a 25°C por 7 dias em agitador orbital a 150 rpm. Foram determinados o teor de proteínas totais e a atividade de celulase como: endoglucanase, exoglucanase e β-glucosidase e ainda xilanase. Os resultados demonstraram que a produção das enzimas hidrolíticas foi estimulada pela presença de alta concentração de bagaço de cana (30g/L), caracterizando-o como agente indutor devido à relação de proporcionalidade demonstrada. Por sua vez, o sulfato de amônio atuou como redutor da síntese de enzimas, sendo as baixas concentrações (0,1g/L) indicadas para o sistema de produção das enzimas em estudo. Quanto às linhagens, a EF52 mostrou maior atividade para xilanase, endoglucanases, β-glucosidase e proteínas.

Preparation and Determination of Encapsulation Efficiency and Content of Bererine Hydrochloride Lipo-somes / 中国药师

Objective:To prepare bererine hydrochloride ( BER) liposomes and establish an effective method for the determination of content and entrapment efficiency. Methods:BER liposomes were prepared by ammonium sulfate gradient method. The encapsula-tion efficiency of BER liposomes was respectively determined by supercentrifugation method, microcolumn gel method and ultrafiltration method, and the content of every component in BER liposomes was detected by HPLC-ELSD. Results:The results showed that super-centrifugation method could precisely separate the free drug from the liposomes. The optimum parameters of supercentrifugation method were the centrifugal speed of 60 000 r·min-1 , the centrifugal time of 1 h, the centrifugal temperature of 10℃ and the lipid concentra-tion of 6 mg·ml-1 . Conclusion:The method is simple and sensitive with good separation efficiency. HPLC-ELSD can be used to de-termine the content of every component in BER liposomes.

Preparation of vinorelbine tartrate liposomes injection by different methods and their properties and antitumor effects / 中国药学杂志

RESULTS: All EEs of the three VLIs were over 93%. The mean diameters, Zeta potentials and entrapment efficiencies of the three VLIs were similar. Meanwhile the three VLIs were stable in the long-term stability test. The cryo-TEM showed that almost all vesicles had spherical structure and uniform nanosize. Furthermore, some colloid substances and acicular crystal were found inside VLI-2 and VLI-3, respectively. The release rate of VLI-3 was slower, which indicated that the drug crystal probably influence the in vitro drug release. The pharmacodynamic test showed that the antitumor effect of VLI-3 was better than VLI-2 and VLI-1, while those of the latter two were superior to VI.

Análise química da folha "D" de abacaxizeiro cv. Smooth Cayenne antes e após a indução floral em função de doses e parcelamentos de nitrogênio / Chemical analysis of " D " leaf of cv. Smooth Cayenne pineaple before and after the flowering induction as a function of doses and splitting of nitrogen

A cultura do abacaxi é exigente em fertilidade do solo, neste sentido a realização da análise química da folha "D" é muito importante para verificar o seu estado nutricional. O objetivo deste trabalho foi avaliar os teores foliares nutricionais antes e após a indução floral em abacaxizeiro Smooth Cayenne, submetidos a doses e parcelamentos de nitrogênio. O delineamento experimental adotado foi de blocos ao acaso com quatro repetições. Para a 1ª época de análise apenas o efeito de dose foi analisado, já para a 2ª época de análise, foram avaliados dezesseis tratamentos em um esquema fatorial 4x4 (doses e parcelamentos de N). A aplicação de nitrogênio antes da indução floral seja de maneira parcelada ou não proporcionou maiores comprimentos, diâmetros e teores de N, K e S nas folhas 'D', e consequentemente uma maior produtividade de frutos. No entanto a aplicação do N, 2/3 antes e 1/3 após a indução floral superou a produção de frutos em 2 t ha-1, comparada a aplicação total de N antes da indução floral, cabendo ao produtor analisar se o parcelamento seria rentável ou não. O incremento das doses de N proporcionou decréscimo apenas para os teores foliares de K antes e após a indução floral, para os outros macronutrientes houve ajuste de equações do tipo linear positiva e/ou quadrática.

The pineapple plant is demanding in soil fertility, so the accomplishment of chemical analysis of "D" leaf is very important to verify its nutritional state. The objective of this work was to evaluate the leaf nutrient content before and after the flowering induction in pineapple cv. Smooth Cayenne, submitted to doses and splitting of nitrogen. A randomized completely blocks design in four repetitions was used. To first analysis time only the dose effect was just analyzed, already to second analysis time, they were appraised sixteen treatments in a factorial scheme 4x4 (doses and splitting of N). The application of nitrogen before of flowering induction parceled or non increase length, diameter and contents of N, K and S in the leaves "D", presenting positive reflexes in the productivity of the fruits. However the application of N, 2/3 before and 1/3 after of flowering induction exceed the fruit production in 2 t ha -1, comparing tothe application of all the dose of N before of flowering induction, belong the producer analyze of the profitability of parceled or not the nitrogen fertilization. The increment on nitrogen doses provided decreasing on K leaf content before and after of flowering induction, for the other macronutrients were fitted to positive linear and/or quadratic equations.

Isolation, purification and characterization of alkali and thermo stable Xylanase from bacillus sp. Ks09.

Nine bacterial strains were isolated using xylan rich media. The bacterial strain KS09 was selected on the basis of qualitative and quantitative test. It was identified as Bacillus sp. via physiological, morphological and biochemical characterization. The xylanase was purified to homogeneity from crude extract of Bacillus sp. KS09 using ammonium sulphate fractioning and CM-Sephadex C-50. The final purification fold was 10.20 with a recovery of 36.18%. The enzyme was optimally active at 50°C, pH 7.0 and stable over a broad pH range of 6.0-11.0. The residual activity at 6.0-11.0 pH was 100% even upto 3 h of incubation. The enzyme showed 75, 70 and 60% thermal stability at 50, 55 and 60°C, respectively after 1 h of incubation. The kinetic parameters (Km 22.59 mM; Vmax 76.93 IU/mL) were estimated using Lineweaver-Burk plot for purified xylanase. The xylanase activity was inhibited by all the metal ions applied. The characteristic studies revealed that xylanase including its cellulase free nature, broad pH stability and temperature stability are particularly suited its industrial applications.

Fontes alternativas à ureia no fornecimento de nitrogênio para o arroz irrigado / Alternative sources of supply in the urea nitrogen for rice production

A ureia é a principal fonte de N usada na agricultura, mas apresenta grandes perdas em arroz irrigado por alagamento. O objetivo deste trabalho foi avaliar a eficiência de fontes de N alternativas à ureia para utilização pelo arroz irrigado aplicadas em diferentes estádios de desenvolvimento da planta através da quantificação da volatilização de N-NH3 e de parâmetros agronômicos. O experimento foi realizado em campo nos anos agrícolas 2007/08 e 2008/09. O delineamento experimental foi de blocos ao acaso com quatro repetições. Os tratamentos foram compostos de diferentes fontes de N aplicados na semeadura, no perfilhamento e no estádio de diferenciação floral: testemunha sem aplicação de N, ureia, ureia+inibidor de urease, duas fontes de N líquido (N-28 e N- 30), sulfato de amônio e ureia + enxofre. Nas duas safras, a volatilização de N-NH3 na semeadura foi praticamente insignificante, enquanto no estádio V4 a fonte que menos volatilizou foi o sulfato de amônio. A resposta em produtividade variou entre os anos, provavelmente em função da disponibilidade de N no solo e aspectos climáticos. Quando vantajoso economicamente, o uso do inibidor de urease e o sulfato de amônio proporcionam produtividades semelhantes à fonte ureia.

Urea is the main source of N used in agriculture, but has large losses in irrigated rice. The objective of this study was to evaluate the efficiency of N sources alternative to urea for use by rice applied at different stages of plant development through the quantification of N-NH3 and agronomic parameters. The experiment was conducted in the field in the agricultural years 2007/08 and 2008/09. The experimental design was randomized blocks with four replications. The treatments consisted of different N sources applied at seeding, tillering and at the stage of floral differentiation: control without N, urea, urea + urease inhibitor, two sources of liquid N (N-28 and N-30) ammonium sulfate and urea + sulfur. In both seasons, the volatilization of N-NH3 at seeding was almost negligible, whereas in the V4 stage, the source that was less volatilized was the ammonium sulfate. Yield response varied among years, probably due to the availability of N in the soil and climatic aspects. When economically advantageous, the use of urease inhibitor and ammonium sulfate provide yields similar to urea supply.

Eficiência nutricional e aproveitamento do nitrogênio pelo capim-marandu de pastagem em estágio moderado de degradação sob doses e fontes de nitrogênio / Nutritional efficiency and nitrogen uptake by capim-marandu at pasture in moderate stage of degradation under doses and sources of nitrogen

O presente estudo foi desenvolvido sob condições de campo, visando avaliar a eficiência no uso e no aproveitamento do nitrogênio pelo capim-marandu por meio de diversos índices. O experimento foi conduzido na Fazenda Modelo da Universidade Estadual de Goiás (UEG) num Latossolo Vermelho distrófico, no período de julho de 2003 a março de 2006. O delineamento experimental utilizado foi de blocos ao acaso, com três repetições. Os tratamentos nas parcelas foram caracterizados por um fatorial 2 x 4, sendo duas fontes de N (sulfato de amônio e uréia) e quatro doses de N (0, 100, 200 e 300 kg/ha). Na subparcela, os tratamentos foram representados pelos anos (2004, 2005 e 2006), referentes ao tempo de recuperação da pastagem. A adubação nitrogenada foi parcelada em três aplicações após cada corte de avaliação da forrageira. Foram realizados três cortes da forrageira por ano, avaliando-se a massa seca e os teores de N nos tecidos. Com esses resultados, avaliaram-se os índices de Eficiência de Utilização do N (EUN), Recuperação do N Aplicado (RNA), Eficiência Agronômica do N Aplicado (EA) e Eficiência Fisiológica (EF) ou Eficiência Biológica. Os resultados mostraram que a EUN aumentou, enquanto que a RNA, EA e a EF diminuíram com o aumento das doses de N aplicadas. Em todos os casos, os índices foram influenciados pelos anos de recuperação da forrageira estudados, mas não pelas fontes de N.

This study was accomplished under field conditions, to evaluate the use and efficiency of nitrogen uptake, through various indexes, in pasture of Brachiaria brizantha. The experiment was conduced at the Model Farm of the Goiás State University in a distrophic Dark Red Latosol, from 2003 July to 2006 March. The experimental design was randon blocks, with three replicates. The treatments were placed in parcels characterized by a 2x4 factorial, with two sources of N (ammonium sulfate and urea) and four doses of N (0, 100, 200 and 300 kg/ha/year). In sub - parcel, the treatments were represented per years (2004, 2005 and 2006) refering to time of pasture recovery. The use of nitrogen was divided in three times, after each cut evaluation of the forage pasture. Three cuts were made per year, evaluating dry mass and N accumulation in the tissue. Nitrogen Utilization Efficiency, Recovery from the N Applied, Agronomic Efficiency from the N Applied, Physiological or Biological Efficiency indexes were evaluated. The results showed that EUN increased, while RNA, EA and EF reduced with the increase in the N doses applied. In all cases, the indexes were influenced by the years of recovery the herbage, and not by the sources of N.

Doses, fontes e épocas de aplicação de nitrogênio em cultivares de arroz / Doses, sources and periods of nitrogen application in rice cultivars

As exigências das culturas pelo nitrogênio são diferenciadas, tanto no que se refere às quantidades, quanto à época de aplicação, sendo que este nutriente pode ser perdido por lixiviação, volatilização e erosão. Conduziu-se este trabalho, com o objetivo de avaliar a resposta das cultivares de arroz BRSMG Curinga e IAC 202 à aplicação de doses, fontes e épocas de aplicação de nitrogênio. Os tratamentos constituíram-se de cinco doses de nitrogênio (0, 50, 100, 150 e 200 kg de N ha-1), sob a forma de três fontes nitrogenadas (sulfonitrato de amônio com inibidor de nitrificação, sulfato de amônio e uréia), em duas épocas de aplicação (semeadura ou perfilhamento), cultivado com irrigação suplementar. O delineamento experimental utilizado foi o de blocos ao acaso em esquema fatorial 5x3x2x2, com quatro repetições. A cultivar de arroz BRSMG Curinga apresentou a maior produtividade. As doses de N aumentaram o teor de N foliar, a altura de plantas, o número de panículas m-2, o número e a massa de espiguetas granadas panícula-1 e a produtividade de grãos, mas não influenciou a massa de 100 grãos. As fontes nitrogenadas e as épocas de aplicação tiveram efeito semelhante para todas as avaliações. A maior produtividade foi alcançada com a dose de 122 kg de N ha-1, independente da fonte utilizada.

Nitrogen is a nutrient that can be lost through leaching, volatilization and erosion. Crops require nitrogen in different doses and application times. The objective of this work was to evaluate the performance of BRSMG Curinga and IAC 202 rice to application of five doses of nitrogen (0, 50, 100, 150 and 200 kg of N ha-1) using three nitrogen sources (ammonium sulfonitrate with nitrification inhibitor, ammonium sulfate and urea), at two times of application (at sowing or at tillering stage), with sprinkler irrigation. A randomized block design was used in a 5x3x2x2 factorial scheme, with four repetitions. The Curinga rice showed the highest grain yield. The doses of N increased the foliar N content, the height of plants, the number of panicles m-2, the number of full grains panicle-1, the mass of full grains per panicle and the grain yield, but did not influence the mass of 100 grains. The time of application and the nitrogen sources had a similar effect in all evaluations. The highest grain yield was reached with a dose of 122 kg of N ha-1, regardless of the source.

Preparation of sophoridine liposomes by ammonium sulfate transmembrane gradient method / 中草药

Objective: To investigate and optimize the formulation of sophoridine liposomes. Methods: Sophoridine liposomes were prepared by ammonium sulfate transmembrane gradient method. On the basis of single factor experiments, the effects of influence factors, such as entrapment efficiency, drug loading, and comprehensive indexes, were investigated by using central composite design and response surface method. The influence factors included the concentration of drug and ammonium sulfate. The data were imitated using multi-linear equation and second-order polynomial equation. Results: The latter was prior to the former considering from multiple correlation coefficients. Under the optimal conditions, the entrapment efficiency and drug loading of sophoridine liposomes were 51.81% and 5.39%. Conclusion: The optimized preparation technique for sophoridine liposomes is stable and feasible.

Manejo da adubação nitrogenada paraa batata-doce: fontes e parcelamento de aplicação / Management of nitrogen fertilization for sweet potato: sources and application parceling

Com o objetivo de avaliar o rendimento da batata-doce [Ipomoea batatas (L.) Lam.], cultivar Rainha Branca, submetida a fontes e parcelamentos da aplicação de 80 kg ha-1 de nitrogênio, instalou-se um experimento, no período de abril a agosto de 2007, na Universidade Federal da Paraíba, em Areia. O delineamento experimental utilizado foi o de blocos casualizados, em esquema fatorial 2 x 7, com duas fontes de nitrogênio (uréia e sulfato de amônio) e oito de épocas parcelamentos da aplicação (100 por cento no plantio; 100 por cento aos 30 dias após o plantio (DAP); 100 por cento aos 60 DAP; 50 por cento no plantio e 50 por cento aos 30 DAP; 50 por cento no plantio e 50 por cento aos 60 DAP; 50 por cento aos 30 e 50 por cento aos 60 DAP; 33 por cento no plantio, 33 por cento aos 30 e 33 por cento aos 60 DAP), com quatro repetições. Foram avaliados o peso médio de raízes comerciais, a produção e o número de raízes comerciais por planta e as produtividades total e comercial de raízes de batatadoce. O maior peso médio de raízes comerciais (294 g) foi obtido com o parcelamento do N, como uréia, 50 por cento no plantio e 50 por cento aos 30 DAP. O parcelamento de N 33 por cento no plantio, 33 por cento aos 30 DAP e 33 por cento aos 60 DAP na forma sulfato de amônio foi responsável pela maior produção de raízes comerciais por planta (337 g). Para o número de raízes comercias e as produtividades total e comercial, o N na forma de sulfato de amônio parcelado 33 por cento no plantio, 33 por cento aos 30 DAP e 33 por cento aos 60 DAP proporcionou os maiores valores, 1,50 raízes, 30,5 e 28,4 t ha-1, respectivamente.

For the purpose of evaluating the yield of the sweet potato [Ipomoea batatas (L.) Lam.], variety "Rainha Branca", subjected to sources and parceling of nitrogen, an experiment was carried out in soil classified as typical "Inceptisol Regolítico Psamítico" at the Universidade Federal da Paraíba, Areia county, from April to August 2007. The experimental design used was one of randomized blocks, with a factorial scheme of 2 x 7, using two sources of nitrogen fertilizer (urea and ammonium sulfate) and eight application times (100 percent at planting; 100 percent at 30 days after planting (DAP); 100 percent at 60 DAP; 50 percent at planting and 50 percent at 30 DAP; 50 percent at planting and 50 percent at 60 DAP; 50 percent at 30 and 50 percent at 60 DAP; and 33 percent at planting, 33 percent at 30 and 33 percent at 60 DAP), with four replicates. The following variables were evaluated: average weight of the commercial roots plan t-1, the total, commercial, and noncommercial productivities of sweet potato roots. The highest average weight of the commercial roots was obtained with urea in parceling 50 percent at planting and 50 percent at 30 DAP, (293.75 g).. Parceling 33 percent at planting, 33 percent at 30, and 33 percent at 60 DAP were responsible for the highest yield of commercial roots per plant. The lowest yield of commercial roots per plant was obtained in the same source with parceling 50 percent at 30 and 50 percent at 60 DAP (26.48 g). For the number of commercial roots, total and commercial productivities, ammonium sulfate parceled 33 percent at planting, 33 percent at 30, and 33 percent at 60 DAP provided the highest values (1.50 roots, 30. and 28.4 t ha-1, respectively.

Preparation and Stability of Tetrandrine Liposomes / 中国药房

OBJECTIVE: To draw a comparison between the two preparative technologies of tetrandrine liposomes. METHODS: Tetrandrine liposomes were prepared with ammonium sulfate transmembrane gradient method and film dispersion method respectively. Leaking ratio,particle size,and content of phospholipid of liposomes were selected as the parameters to indicate the stability of liposomes,and the entrapment efficiency was taken as quality index to compare the two preparation technologies. RESULTS: Tetrandrine liposomes prepared with ammonium sulfate transmembrane gradient technology achieved high entrapment efficiency of 81.1% and good stability,while those prepared by film dispersion method had an entrapment efficiency of only 32.9% with poor stability. CONCLUSION: Ammonium sulfate transmembrane gradient method is feasible and superior to the film dispersion method in the preparation of tetrandrine liposome.

THE GLUCOSE OXIDASE-DAB-NICKEL METHOD IN PEROXIDASE HISTOCHEMISTRY OF THE NERVOUS SYSTEM / 解剖学报

A glucose oxidase-3,3′ diaminobenzidine-nickel method was developed.Thistechnique can successfully demonstrate the details of the immunoreactive structuresand PHA-L labeled cell bodies and their processes.It is especially beneficial forvisualizing fibers and terminals.It is more sensitive than the regular 3,3′ diamino-benzidine method and the glucose oxidase-3,3′ diaminobenzidine technique,andvery stabilized.