ABSTRACT
Five compounds were isolated from the alcohol extract of Olibanum by MCI, silica gel, ODS, and Sephadex LH-20 column chromatographies and preparative high-performance liquid chromatography(HPLC). On the basis of spectral data and literature data, the compounds were identified as:(1S,3R,4S,7R,11S,12R)-1:12,4:7-diepoxisonane-8(19)-ene-3,11-diol(1), boscartin A(2),(+)-resinolin(3),(+)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(4), and acerogenin A(5). Compound 1 is a new compound, and compounds 3-5 were isolated from Olibanum for the first time. The structure of compound 1 was determined by spectroscopic analysis and single-crystal X-ray diffraction. Compounds 1 and 2 were tested for PC12 neurotoxicity, and the results showed that they were both safe compounds.
Subject(s)
Chromatography, High Pressure Liquid , Diterpenes , Frankincense , Molecular StructureABSTRACT
This study aims to study the chemical components from the gum resin of Boswellia carterii. Five cembranoid diterpenes were isolated from the gum resin of B. carterii by various of column chromatographies including silica gel, Sephadex LH-20, and semi-preparative HPLC. Their structures were identified on the basis of physicochemical properties, mass spectrometry(MS), nuclear magnetic resonance(NMR), Ultraviolet(UV) and infrared(IR) spectroscopic data. These compounds were identified as(1S,2E,4R,5S,7E,11E)-4-methoxy-5-hydroxycembrane(1),(1R~*,4R~*,5E,8E,12E,15E)-4-hydroxycembra-5,8,12,15-tetraene(2), cembrene A(3),(3S,4S,7R)-4-hydroxycembrane(4), and pavidolide D(5). Compound 1 was a new compound. Compounds 2, 4, and 5 were obtained from the gum resin of B. carterii for the first time. Compound 2 showed weak inhibition on the human liver cancer cell line HepG2.
Subject(s)
Humans , Boswellia , Cell Line , Diterpenes , Molecular Structure , Resins, PlantABSTRACT
Objective To study the chemical constituents from the twigs of Euonymus alatus. Methods Compounds were isolated and purified by a combination of various chromatographic techniques, and their structures were elucidated by physiochemical property and spectral analysis. Results Three compounds were obtained from 95% ethanol extract of the twigs of E. alatus and identified as (1S,2S,7E,11R,12S)-2,11-dihydroxy-1,12-oxidocembra-4(18),7(8)-diene (1), hemerocallal A (2), and betulinic acid methyl ester (3). Conclusion Compound 1 is a new cembranoid diterpene and named euonymuditerpene A, and compounds 2 and 3 are isolated from the twigs of Euonymus alatus for the first time.
ABSTRACT
Objective: To study the cembranoid diterpenes from the leaves of Nicotiana tabacum. Methods: The cembranoid diterpenes were isolated and purified by silica gel and preparative HPLC, and their structures were elucidated on the basis of their physicochemistry properties and spectra data. Results: Five cembranoid diterpenes were obtained from the ethanol extract of the leaves of N. tabacum and identified as (1R, 3E, 7E, 10S, 11E)-3,7,11-cembratriene-10, 15-diols (1), (1S, 2E, 4S, 6R, 7E, 10E, 12S)-2, 7, 10- cembratriene-4, 6, 11-triol (2), (1S, 2E, 4R, 6R, 7E, 10E, 12S)-2, 7, 10-cembratriene-4, 6, 11-triol (3), (1S, 2E, 4S, 6R, 7E)-4, 6, 11-trihydroxy- 1-isopropyl-4, 8-dimethylpentadeca-2, 7-dien-12-one (4), and (1S, 2E, 4R, 6R, 7E, 11S, 12S)-11, 12-epoxy-2, 7-cembradiene-4, 6-diol (5). Conclusion: Compound 1 is a new cembranoid diterpene and named as nicotiaditerpene B, and compound 4 is a new natural product.
ABSTRACT
Objective: To study the chemical constituents from the aerial parts of Euphorbia altotibetica. Methods: The compounds were isolated and purified by various chromatographic techniques and their structures were elucidated by spectral analysis. Results: Six compounds were isolated and their structures were identified as altotibetin E (1), proline (2), corchoionoside C (3), icariside B2 (4), 1-O-(9Z,12Z,15Z-octadecatrienoyl)-2-O-hexadecanoyl-3-O-α-(6-sulfoquinovopyranosyl) glycerol (5), and (2S)-2,3-O-dioctadeca-9Z, 12Z,15Z-trienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (6). Conclusion: Compound 1 is a new cembranoid diterpene named altotibetin E, compounds 2, 5, and 6 are isolated from the species of Euphorbia L. for the first time and the other compounds are firstly obtained from this plant.