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Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health functions. The purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from 'Brightwell' rabbiteye blueberries in mice. After one week of adaptation, C57BL/6J healthy male mice were divided into different groups that were administered with 100, 400, or 800 mg/kg blueberry anthocyanin extract (BAE), and sacrificed at different time points (0.1, 0.5, 1, 2, 4, 8, or 12 h). The plasma, eyeball, intestine, liver, and adipose tissues were collected to compare their antioxidant activity, including total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity and glutathione-peroxidase (GSH-PX/GPX) content, and the oxidative stress marker malondialdehyde (MDA) level. The results showed that blueberry anthocyanins had positive concentration-dependent antioxidant activity in vivo. The greater the concentration of BAE, the higher the T-AOC value, but the lower the MDA level. The enzyme activity of SOD, the content of GSH-PX, and messenger RNA (mRNA) levels of Cu,Zn-SOD, Mn-SOD, and GPX all confirmed that BAE played an antioxidant role after digestion in mice by improving their antioxidant defense. The in vivo antioxidant activity of BAE indicated that blueberry anthocyanins could be developed into functional foods or nutraceuticals with the aim of preventing or treating oxidative stress-related diseases.
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Male , Mice , Animals , Antioxidants/pharmacology , Blueberry Plants , Anthocyanins/pharmacology , Mice, Inbred C57BL , Superoxide Dismutase , Plant Extracts/pharmacology , Superoxide Dismutase-1ABSTRACT
Aim To investigate the effects of astragalus polysaccharide (APS) on depressive behaviors, hippocampal damage and Nrf2-ARE signaling pathway in rats. Methods Wistar rats were randomly divided into control group, depression group, APS low dose group and APS high dose group. Rats (except the control group) underwent chronic unpredictable mild stress (CUMS) for 28 days. The depressive behaviors were assessed by tail suspension test, forced swim test and sucrose preference test. The histopathological changes of the hippocampus were valuated by HE staining. Levels of nuclear factor erythroid 2-related factor 2 (Nrf2) protein and Nrf2 mRNA were measured. The hippocampal levels of oxidative stress were evaluated. Results Compared with the control group, the depression group showed significant depressive behaviors and hippocampal damage. The depression group had higher levels of Nrf2 and MDA, but lower levels of HO-1, SOD, CAT and GSH-Px than the control group. However, APS does-dependently attenuated the hippocampal damage and depressive behaviors, increased hippocampal levels of Nrf2, HO-1, SOD, CAT and GSH-Px, but decreased hippocampal levels of MDA in rats. Conclusions APS can attenuate CUMS-induced hippocampal damage and depressive behaviors in rats, and the effects may be associated with the activation of Nrf2-ARE signaling pathway.
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Objective:To study the effect of Schisandrae Chinensis Fructus lignans (SCL) on learning and memory ability of D-galactose（D-gal）-induced aging model mice. Method:ICR mice were randomly divided into 4 groups: normal group (distilled water, subcutaneous injection with normal saline), model group (distilled water, subcutaneous injection with 200 mg·kg-1D-gal), piracetam group (oral administration with 200 mg·kg-1 piracetam, subcutaneous injection with 200 mg·kg-1D-gal), low-dose SCL group (oral administration with 50 mg·kg-1·d-1 SCL, subcutaneous injection with 200 mg·kg-1·d-1 D-gal), medium-dose SCL group (oral administration with 100 mg·kg-1·d-1 SCL, subcutaneous injection with 200 mg·kg-1·d-1D-gal), high-dose SCL group (oral administration with 200 mg·kg-1·d-1 SCL, subcutaneous injection with 200 mg·kg-1·d-1 D-gal). The drugs were administered continuously for 10 weeks. Dark test and Morris water maze test were performed to observe the effect of SCL on the learning and memory ability of D-gal-induced aging mice. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in mouse brain tissue were detected by chemical colorimetry. The expressions of peroxiredoxin-6（Prdx6） and glutathione peroxidase 1（GSH-Px1） mRNA in mouse brain tissue were detected by polymerase chain reaction (PCR). The expressions of Prdx6 and GSH-Px1 protein in mouse tissues were detected by Western blot. Result:In behavioral experiments, compared with normal group, the number of dark avoidance errors in model group significantly increased (P<0.05), the latency was significantly reduced (P<0.01), and the number of mouse passes and the target quadrant residence time were significantly reduced (P<0.01), which can be used as an indicator of successful modeling. Compared with the model group, the number of errors in the piracetam group, and medium and high-dose SCL groups was significantly reduced (P<0.05，P<0.01), and the latency was significantly prolonged (P<0.05，P<0.01). At the same time, the number of water maze passes and the target quadrant retention time in the high-dose SCL group increased significantly (P<0.01). The results of biochemical indicators showed that compared with normal group, the SOD activity in brain tissue of model group mice was significantly reduced (P<0.01), while the MDA content was increased (P<0.05). Compared with the model group, SOD activity in the brain tissues of piracetam group, and low, medium and high-dose piracetam groups was significantly increased (P<0.05), whereas the level of MDA was reduced (P<0.05). The expressions of Prdx6 and GSH-Px1 were significantly increased (P<0.05，P<0.01), indicating that the SCL administration group was dose-dependent. Conclusion:SCL can improve the learning and memory ability of D-gal-induced aging mice, which may be related to the anti-oxidation ability of SCL and the up-regulation of Prdx6 and GSH-Px1 expressions in mouse brain tissue.
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Present study was aimed at investigating the effects of threonine supplementation on antioxidant enzyme activities and haemato-biochemical profile of commercial broilers in sub-tropics. Three hundred thirty -day old straight run commercial broiler chicks (Vencobb-400) with initial average body weight of 44.04±0.42g were allocated into five experimental groups, in a completely randomized design (CRD) with 42 days experiment. Groups were formed according to the dose of supplemental L-threonine in various rations i.e. NRC specification, 100% of Vencobb-400 strain specification, 110% of Vencobb-400 specification, 120% of Vencobb-400 specification and 130% of Vencobb-400 specification group. The mean serum GSH-Px and serum catalase concentration increased linearly {(p=0.001) and (p=0.04), respectively} whereas the mean serum SOD level increased both linearly (p=0.002) and quadratically (p=0.04) with the increasing levels of supplemental L-threonine. Among the hematological parameters of blood, the H:L ratio decreased linearly (p=0.02) with the increasing levels of threonine. The serum glucose and total protein concentration increased linearly (p=0.002) with the increasing levels of supplemental L-threonine. There was a linear increment (P<0.001) in serum globulin level with a linear decrease (p<0.05) in albumin: globulin ratio on increased levels of supplemental L-threonine in the ration. There was a linear decrease (p<0.001) in cholesterol and VLDL level with the increasing levels of supplemental L-threonine, however, a linear increment (p=0.04) in the serum HDL level was noticed. It may be concluded that L-threonine supplementation at 130% threonine (of Vencobb-400 specification) has a better antioxidant function and better haemato-biochemical profile
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Objective To investigate the effect of 1 800 MHz electromagnetic radiation on activity of SOD and GSH－Px in the skin tissues of SD rats.Methods A total of 98 healthy SD rats with SPF level,aged 4 weeks, were randomly divided into radiation group and control group.The radiation group was totally exposed under 1 800 MHz electromagnetic wave with seven different power density of radiation of 0.1 mW/cm2,0.3 mW/cm2,0.5 mW/cm2 , 0.7 mW/cm2, 0.9 mW/cm2, 1.0 mW/cm2and 1.2 mW/cm2respectively.It lasted 21 days and for a period of 12 hours a day. After radiation,the activity of SOD and GSH－Px in the skin tissues were detected by enzyme marker. Results In radiation group,the activity of SOD and GSH－Px in the skin tissues of SD rats were decreased under 0.3 mW/cm2and 0.5 mW/cm21 800 MHz electromagnetic wave. Compared with the control group, there was a significantly difference in radiation group (P＜0.05) .While under other four 1 800 MHz electromagnetic waves, the activity of GSH－Px and SOD in the skin tissues showed no statistical difference between the two groups (P＞0.05) . Under 1 mW/cm21 800 MHz electromagnetic wave, the activity of GSH－Px showed no statistical difference between two groups (P＞0.05) . Conclusion The power density of 0.3 mW/cm2and 0.5 mW/cm21 800 MHz electromagnetic wave can reduce the activity of GSH－Px and SOD in the skin tissues of rats.
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Objective To investigate the effect of baicalein on mouse ovarian injury induced by triptolide. Methods Female healthy BALB/C mice were randomized into four groups. The mice in control group were given water contained 5% DMSO. The mice in model group were given 25 μg/kg triptolide orally. While the mice in baicalein treated groups were given 25 μg/kg triptolide plus 100 mg/kg and 500 mg/kg baicalein, respectively. On the 41st d of experiment, continuous vaginal smear were made to measure the sexual cycle of mice. On the 47th d, the superovulation experiment was carried out. On the 50th d, the mice were sacrificed to collect the ovaries. Ovaries were examined by hematoxylin and eosin (HE) staining, and the levels of follicles were counted. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in the ovarian tissue of each group were measured to evaluate the level of oxidative stress. Western blotting assay was performed to measure the expression levels of thioredoxin reductase (TrxR), thioredoxin (Trx), glutathione reductase (GR), and GSH-Px. Results Triptolide significantly induced ovarian damage, reduced the number of follicles, induced ovarian interstitial connective tissue regional osteoporosis, and decreased the activities of SOD and GSH-Px, while increased the content of MDA. On the contrary, when adding baicalein, the mice ovary development was obviously improved. The follicle cell number increased, and the connective tissue in interstitial region of ovary was well-stacked. The activity of SOD and GSH-Px in ovarian tissue was increased and the content of MDA was decreased. Western Blotting results showed that the expression levels of TrxR in model group mice were significantly down-regulated compared with those of control group. However, compared with control group, the expression levels of TrxR were significantly up-regulated by baicalein treatment. Conclusion Baicalein can enhance the anti-oxidative ability of mouse ovarian tissue by up-regulating TrxR expression, and may play an important role in the protection of ovarian injury induced by triptolide.
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Objective: To observe the effect of the administration temperature of GQD on the efficacy to ulcerative colitis (UC) of rats. Methods: The GQD was given to dextran sulfate sodium (DSS)-induced UC rats at different temperature (10℃/42℃) for 7 d. The disease activity index (DAI), histopathological score of colon, superoxide dismutase (SOD), malondialdehyde (MDA), GHS-Px, myeloperoxidase (MPO) of serum and colon tissue, and the tumor necrosis factor (TNF-α) of serum were measured and observed. Results: Compared with the control group, the UC relative symptom and index changes of rats in the model group appeared obviously. GQD showed the significant therapeutic effects in both 10℃ and 42℃ groups. The lower temperature administration achieved significantly better results than the higher one on the histopathological score, colon wall thickness, and biochemical indices. Conclusion: The cold GQD shows higher efficacy than the hot GQD when treating UC, which may be attributed to the immune response of intestinal mucosal mast cells to temperature-induced stress.
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Aim To determine the neuroprotective effect of sulfated pachymaran (SP)on MPTP-induced mouse model.Method ICR mice were randomly di-vided into control group,MPTP group and SP treatment group (50,1 00,1 50 mg·kg -1 ,ip).After 1 7 days, the activities of endogenous antioxidants (SOD,GSH-Px,CAT),antisuperoxide anion,hydrogen peroxide and MDA content in the midbrain and cortex were as-sayed.Results The results proved that SP significant-ly reduced the content of MDA and H2 O2 ,regulated the activities of antioxidant enzyme and increased the activity of antisuperoxide anion.Conclusion All these effects indicate that SP is a potential neuroprotective a-gent and its neuroprotective effects are achieved in the MPTP mouse model.
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OBJECTIVE:To study the antioxidant effects of Scutellaria baicalensis(SBE)on the model mice with Alzheimer’s disease. METHODS:All mice were treated by D-gal (150 mg/kg) and NaNO2 (100 mg/kg) for 60 d to reproduce the AD model mice,ip,once a day. Totally 60 mice were randomly divided into normal control group(isovolumetric sodium chloride injection), model group (isovolumetric sodium chloride injection),estradiol (0.4 mg/kg) group,and SBE high,medium and low doses (500,250 and 125 mg/kg). The mice were intragastrically administrated while modeling except normal control group,once a day, for continuous 60 d. The superoxide dismutase(SOD),catalase(CAT)activities and malondialdehyde(MDA)content in brain tis-sue ,and glutathione peroxidase (GSH-Px) activity in serum of mice were determined. RESULTS:Compared with normal control group,the SOD,CAT activities in brain tissue,and GSH-Px activity in serum of mice in model group were decreased,MDA con-tent in brain tissue were increased,with significant difference(P<0.01 or P<0.05);compared with model group,the SOD,CAT activities in brain tissue and GSH-Px activity in serum of mice in SBE high and medium dose groups were increased,MDA content was decrease,with significant difference (P<0.01 or P<0.05). CONCLUSIONS:SBE can enhance the antioxidant capacity in AD model mice by a mechanism that may be related to the improvement of related oxidation indicators’levels.
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Objective To observe the effects of thin recipe ofBuyang Huanwu Decoction on expressions of glutathione (GSH) antioxidant system including GSH, GSH-Px andγ-GCS in the brain of focal cerebral ischemia rats;To study its mechanism of antioxidant effect.Methods Totally 120 SD rats were randomly divided into four groups according to random number table method:sham-operation group, model group,Buyang Huanwu Decoction group and thin recipe ofBuyang Huanwu Decoction group. Except for the sham-operation group, the rest groups established focal cerebral ischemia rat model by middle cerebral arterial occlusion. The treatment groups were given corresponding medicine by gavage, while the sham-operation group and model group were given the same amount of normal saline 2 h after modeling. Each group was detected after cerebral ischemia for 1 day, 3 days, and 7 days respectively. The content of GSH and the activity of GSH-Px were detected. At the same time,γ-GCS mRNA and protein levels were determined by using real time-PCR and Western blot.Results The content of GSH and the activity of GSH-Px at each time point decreased in the model group (P<0.05), compared with the corresponding sham-operation group. But the expression ofγ-GCS mRNA and protein increased, with statistical significance on day 1 (P<0.05). Compared with model group, content of GSH and GSH-Px activity levels increased differently in each treatment group, whileγ-GCS mRNA and protein expression raised, with statistical significance on day 3 (P<0.05).Conclusion The thin recipe ofBuyang Huanwu Decoction plays antioxidant effect by regulating the expression of glutathione antioxidant system GSH, GSH-Px andγ-GCS after cerebral ischemia, which may be one of its therapeutic mechanisms for cerebral ischemia.
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This article was aimed to study the therapeutic material basis of Xiong-Shao (XS) decoction on hepatic fi-brosis (HF), and screen effective parts from XS decoction for protecting liver, reducing enzyme activity and oxidative damage. Male wistar rats were randomly divided into the normal group, model group, Fu-Zheng Hua-Y u (FZHY) group, XS group, polysaccharide group, total alkaloids group and the total glycosides group. HF rat model was estab-lished with the intraperitoneal injection of DMN. After modeling, FZHY solution (0.105 g·mL-1), XS decoction (1.610 g·mL-1), crude polysaccharides extract of XS decoction (35.420 mg·mL-1), total glycosides extract solution (25.725 mg·mL-1), and total alkaloids extract of XS decoction (0.196 mg·mL-1) were administered to corresponding treatment group by gavage once a day for 4 weeks, respectively. Rats of the normal group and model group were given equiva-lent normal saline by gavage once a day for 4 weeks. After 4-week drug administration, rats were killed to remove the liver. Automatic biochemical analyzer was used in the detection of serum parameters of liver function, including ALT, AST, TIBL and ALB. Serum SOD activity was detected by xanthine oxidase. GSH-PX activity was tested by DTNB reduction. Serum contents of MDA were measured by TBA. Pathological changes of the liver were observed with HE staining and masson staining. The results showed that compared with the model group, there was no signifi-cant differences between the total alkaloids group and the model group, but levels of serum ALT, AST and TBIL of other treatment groups were significantly decreased, and the serum ALB level was significantly elevated (P< 0.05 or P < 0.01). Compared with the model group, levels of serum SOD and GSH-PX of the FZHY group, XS group and total alkaloids group were significantly elevated (P < 0.01), and level of serum MDA was significantly reduced (P <0.05 or P< 0.01). Comparison among the polysaccharides group, total glycosides group, and model group showed no significant differences. It was concluded that crude polysaccharide and total glycosides fractions were effective parts of XS decoction for protecting liver and reducing enzyme activity. And total alkaloids fraction was the effective part for reducing oxidative damage.
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The objective of this study was to evaluate the effect of short-term levosimendan exposure on oxidant/antioxidant status and trace element levels in the testes of rats under physiological conditions. Twenty male Wistar albino rats were randomly divided into two groups of 10 animals each. Group 1 was not exposed to levosimendan and served as control. Levosimendan (12 µg/kg) diluted in 10 mL 0.9% NaCl was administered intraperitoneally to group 2. Animals of both groups were sacrificed after 3 days and their testes were harvested for the determination of changes in tissue oxidant/antioxidant status and trace element levels. Tissue malondialdehyde (MDA) was significantly lower in the levosimendan group (P < 0.001) than in the untreated control group and superoxide dismutase and glutathione peroxidase (GSH-Px) levels were significantly higher in the levosimendan group (P < 0.001). Carbonic anhydrase, catalase and GSH levels were not significantly different from controls. Mg and Zn levels of testes were significantly higher (P < 0.001) and Co, Pb, Cd, Mn, and Cu were significantly lower (P < 0.001) in group 2 compared to group 1. Fe levels were similar for the two groups (P = 0.94). These results suggest that 3-day exposure to levosimendan induced a significant decrease in tissue MDA level, which is a lipid peroxidation product and an indicator of oxidative stress, and a significant increase in the activity of an important number of the enzymes that protect against oxidative stress in rat testes.
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Animals , Male , Rats , Antioxidants/pharmacology , Hydrazones/pharmacology , Malondialdehyde/metabolism , Oxidative Stress/drug effects , Pyridazines/pharmacology , Reactive Oxygen Species/metabolism , Trace Elements/analysis , Glutathione Peroxidase/metabolism , Random Allocation , Rats, Wistar , Superoxide Dismutase/metabolismABSTRACT
Objective: To investigate the stamina,the role of antioxidant system in severe acute pancreatitis(SAP),and the effect of fish oil treatment in rats.Methods:Thirty-six male Sprague-Dawley rats(SD) were randomly divided into three groups,including SAP group(sodium chloride treatment ) (group NSG,n=12),fish oil treatment group (group FOG,n=12),and control group (group CG,n=12).SAP was induced by intergraded injection of 3.5% sodium tanrocholate to biliopancreatic duct of SD rats in group NSG and FOG.The group NSG rats were treated by subcutaneous injection of sodium chloride,while the group FOG rats were treated by subcutaneous injection of fish oil for 7 days.Then the improved tail suspension test was observed at the first,third,fifth,seventh day.12 rats in each group were respectively sacrificed after 7 day.The activity of serum antioxidant enzymes (MDA,GSH-PH) and the concentration of serum amylase were measured in each group,and the levels of threshold on the area and the total immobility time were measured in each group.The severity of pancreatitis was analyzed according to the histopathological morphology.Results: Compared to group NSG,the severity of pancreatitis was significantly decreased in group FOG.The activity of MDA was significantly increased in group NSG than that in group CG (P<0.01) ,while the activity of MDA in group FOG was decreased than that in group NSG (P<0.05) .The activity of GSH-PH was significantly decreased in group NSG than that in group CG(P<0.01),while the activity of GSH-PH was increased in group GOG than that in group NSG (P<0.05).THE level of threshold on the area was decreased in group NSG than that in group CG(P<0.01),while the level of threshold on the area was increased in group GOG than that in group NSG.(P<0.01) The total immobility time was significantly increased in group NSG than that in group CG(P<0.01),while the total immobility time was decreased in group GOG than that in group NSG(P<0.05).MDA was associated with the level of threshold on the area and the total immobility time.Conclusion: Fish oil has a positive effect on the activity of antioxidant system and behavior character in SAP rats.
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Objective To study the effect of Busui Jiannao Prescription on ethology, GSH content and GSH-PX activity in brain of PD model rats. Methods Forty-five the aging pure C57BL rats were divided into a negative injury group, a model group, and a Chinese medicine group. PD animal model were established by injection with MPTP and were treated with Busui Jiannao Prescription by intragastric administration. Score changes of PD model rats in the test of pole-climbing and suspending were observed. GSH content and GSH-PX activity were further measured. Results After treating with Busui Jiannao Prescription, the test scores in pole-climbing and suspending were improved, GSH content and GSH-PX activity in substantia nigra were increased. Conclusion Busui Jiannao Prescription can regulate the behavior coordination and repair the damage of dopaminergic neurons in the substantia nigra of PD model rats.
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Smoking has been known to exacerbate the initiation and propagation of oxidative stresses. Efforts have been made to reduce the smoking-induced oxidative stresses using commercial dietary supplements. Propolis is the resinous substance collected by bees from the leaf buds and bark of trees, especially poplar and conifer trees. In this trial, we examined whether a daily supplementation of 800 mg propolis can protect endogenous lymphocytic DNA damage and modulate antioxidative enzyme activities and the level of antioxidant vitamin in smokers using a placebo-controlled, double-blinded cross-over trial. After two weeks of running-in period, 29 smokers (mean age 34.38 +/- 1.73) received 6 tablets/day of either propolis or placebo pills for 4 weeks. After 2 weeks of washout period the subjects switched they pills for cross-over study. The degree of DNA damage (assessed by tail DNA, tail length and tail moment) was not significantly changed with propolis intake or placebo intake. Similarly, total antioxidant status (TAS) remained at the same level regardless of the treatment. Erythrocyte catalase, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), plasma vitamin C and tocopherol level did not differ before and after propolis treatment, and did not differ between treatments. Putting all these results together, we would suggest that it is still too early to claim that propolis possess antioxidative activities.
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Ascorbic Acid , Bees , Catalase , Tracheophyta , Cross-Over Studies , Dietary Supplements , DNA , DNA Damage , Erythrocytes , Glutathione Peroxidase , Lymphocytes , Oxidative Stress , Plasma , Propolis , Smoke , Smoking , Superoxide Dismutase , Tocopherols , Trees , VitaminsABSTRACT
[Objective] To discuss mechanism of FS-1272 eye drops on cataract of rats induced by D-galactose.[Method]By using the model of cataract of rats induced by D-galactose to determine the content of SOD,CAT,GSH-px,MDA in lens.[Result]FS-1272 eye drops can increase the activities of SOD,CAT,GSH-px and decrease the content of MDA.[Conclusion]FS-1272 eye drops have the effect of delaying the generation and development of cataract by alleviating the extent of oxidative injury of lens.
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@#ObjectiveTo explore the changes of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) in the brain of mice after irradiated by electromagnetic radiation (EMR) and therapeutic effect of curcumin.Methods40 mice were divided randomly into the bare control group, simply EMR group, EMR+curcumin low dose, middle dose and high dose groups total 5 groups with 8 animals in each group. The mice except the bare control group received EMR irradiation and those in the EMR+curcumin groups were given various doses of curcumin at the same time. Five days later, EMR irradiation and medication stopped, and the levels of SOD, GSH-Px and MDA in the brain of mice were tested.ResultsCompared with the bare control group, the activities of GSH-Px and SOD, and level of MDA all increased in the mice irradiated by EMR ( P<0.05). Compared with the simply EMR group, the activities of GSH-Px and SOD, and level of MDA all decreased in the mice of EMR +curcumin groups ( P<0.05).ConclusionEMR irradiation can induce changes of GSH-Px and SOD and peroxidation of mice brain, curcumin can lighten these damages by its anti-oxidation with a dose-dependent effect.
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Objective To explore the relationship between learning and memory ability and antioxidant enzyme (SOD, CAT, GSH-Px)activities in specific brain areas of D-galactose model rats. Methods The aging models were induced by D-galactose in 3-month old rats, and the learning and memory ability of aging model was detected in model MG-2 maze. The activity of SOD, CAT and GSH-Px in brain cortex, cerebellum,hippocampus, striatum and hypothalamus were detected immediately after rats were executed. Results The learning and memory ability of D-ga-lactose aging model was lower than that of control group. The difference of antioxidant enzyme activity among different rat brain areas was significant. The correct response rate of D-galactose aging model was( 58.9 ± 5.4 ) %, which is significantly lower than that of the control group (66.8 ±8.9) %, P ＜ 0.05. The times of response required reaching the standard after 24h of aging model rats was significantly higher than that of the control( Aging group :29.5 ± 12.8, Control group 16.6 ± 6.2; P＜ 0.01 ). Correct response rate after 24h was significantly lower than that of the control ( Aging group :67.3 ± 10.3 , Control group: 79.1 ± 6.9; P＜ 0.01 ). The SOD activity in cortex, hippocampus and striatum( Aging group :66.12 ±5.89,80.46 ± 6.57,87.82 ± 6.87; Control group :57.63 ± 4.21,67.46 ± 6.80,68.37 ± 6.52) significantly reduced ( P ＜ 0.05 and P ＜ 0.01 ).The CAT activity in cortex, hippocampus, striatum and hypothalamus( Aging group :6.45 ± 0.55,5.86 ±0.35,6.93 ± 0.63,8.89 ± 0.38; Control group :5.32 ± 0.56,4.76 ± 0.38,4.37 ± 043,6.11 ± 0.37) significantly reduced ( P ＜ 0.05 and P ＜ 0.01 ). GSH-Px activity was similar in each brain area without significance ( P ＞ 0.05 ). Conclusions The learning and memory ability of aging mode rats decreased. The change of antioxidant enzyme activities in hippocampus, cortex, striatum and hypothalamus is closely related to learning and memory ability.
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[Objective] To study the protection and mechanism of mica granules to rats stomach mucosa injury caused by non-water ethanol.[Method] Randomly divide 48 healthy SD rats into high-,middle-and low-dosed groups of mica granules,Simida group,model control group and blank control group as well,i.e.6 groups.After fasting for 24h,separately make stomach perfusion of mica granules and Simida to all rats in advance;after 3h,make stomach perfusion of non-water ethanol 1ml/100g for stomach mucosa injury;45m later,take proper blood of lower vena cava,sacrifice them for stomach,then respectively test all mucosa injury index,SOD,MDA,and GSH-Px content in serum and mucosa,observe pathological changes to evaluate mucosa injury degree and medical function.[Result] In mica groups,the SOD and GSH-Px were markedly higher than that in model control group,but MDA was lower than later(P
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[Objective]To explore the effect of combined acupuncture and medicine on learning--memory abilities,and free radical system of hippocampus in vascular dementia(VD)rats.[Methods]The 48 Sprague Dawley rats were randomly divided into control(1),VD model(2),VD plus removed olfactory bulb model(3)and XIU Three Needles(4),Eugenol(5),combined acupuncture and medicine(6)groups.Morris maze tests were conducted for valuating the learning and memory abilities.The contents of MDA,SOD and GSH-Px activity of hippocampus were detected.[Results]In comparison with group 1,the average escape latency and travel distance prolonged significantly in group 2.In comparison with group 2,it shortened remarkably in groups 4 and 5.No significant differences were found between groups 1 and 2.In comparison with group 6,it prolonged in groups 4 and 5.No significant differences were found between them.The MDA content of hippocampus in VD model group was higher than that of control group(P