Progress of immunologic derangement in the pathogenesis of primary nephritic syndrome / 国际儿科学杂志

Help T cell 1/Help T cell 2 and some cytokines disequilibrium can give a suitable explanation for hypersensitivity and hypogammaglobulinemia in primary nephritic syndrome(PNS) patients. The disturbance of regulatory T cell(Treg cell) and Th17 cell can lead to correlated cytokines derangement, which explained the pathogenesis of PNS from another aspect. Refractory nephrotic syndrome can be effectively treated by rituximab followed the percentage of regulatory T cell increasing, which indicated that Treg may play an important role in pathogenesis of PNS.

Changes of Activity of Peripheral Blood Mononuclear Cells Nuclear Factor-?B in Children with Primary Nephrotic Syndrome and the Invention of Astragalus / 实用儿科临床杂志

Objective To investigate the pathogenesis of peripheral blood mononuclear cells(PBMC) nuclear factor kappa-?B(NF-?B) in children with primary nephritic syndrome(PNS) and the effect of astragalus on the activity of NF-?B.Methods Twenty-five children with PNS and 20 normal children were studied.Isolated PBMC were separated from 5 mL venous blood in asepsis condition.NF-?B stimulator,NF-?B inhabitor and astragalus were added into the different tubes of PBMC,respectively.The nuclear protein was extracted from the pellets and the optical density(A) values of nuclear protein was measured by enzyme-linked immunosorbent assay(ELISA).Results The activity of PBMC NF-?B in PNS group was higher than that in normal group(P0.05).Astragalus could decrease the activity of PBMC NF-?B which had been stimulated by interleukin-1?(IL-1?)(P

Correlation of Genetical Point Mutation of Platelet Activating Factor Acetylhydrolase and Primary Nephritic Syndrome / 实用儿科临床杂志

Objective To release the correlation of point mutation of platelet activating factor acetylhydrolase(PAF-AH)gene and primary nephritic syndrome (PNS).Method According to the effect of hormonal therapy,94 children with PNS were divided into three groups:steroid-sensitive nephritic syndrome(SSNS),steroid-resistent nephritic syndrome(SRNS),steroid-dependent nephritic syndrome(SDNS).The point mutation of PAF-AH gene (G994T) were identified by molecular biology technique in children with PNS and 239 healthy children were set as control group.Results No statistics differences were found relating to the genotype and allele frequencies between patients with PNS,SSNS,SRNS and normal controls.But it is confirmed that the genotype and allele frequencies among patients with nephritic type nephritic syndrome (NTNS)was higher than patients with simple type nephritic syndrome(STNS) and normal controls.SDNS was higher than both SSNS and normal controls.The number of relapses during the first year after onset was significantly higher in the patients who were heterozygous for the mutant allele (GT) or homozygotes (TT) than in those of the GG homozygotes.Conclusion Most PNS children with PAF-AH gene mutation occurred at position 994 were NTNS.The risk of relapse during the treatment period was higher in patients with PAF-AH gene mutation occurred at position 994.

Study on Detection of Immunoglobulin and Glucocorticoid Administration in Children with Nephrotic Syndrome / 实用儿科临床杂志

Objective To examine serum levels of IgG, IgA, IgM, IgE and C3 and explore sensitivity of glucocorticoid (prednisone) in primary nephropathyic children to study pathogenesis of primary nephropathy and direct clinical therapy. Methods Examine serum IgG, IgA, IgM and C3 by scattering turbidimetry and IgE by ELISA. Results Compared with healthy children, children suffering from primary nephropathy had lower IgG and higher IgM and IgE, but the same IgA and C3 Simple and nephritic nephropathy had very significant difference in sensitivity of glucocorticoid(x2=18.48 P

THE RELATIONSHIP BETWEEN THERAPEUTIC EFFECT OF“TRIPLEX THERAPY”AND PATHOLOGICAL TYPES IN PRIMARY NEPHROTIC SYNDROME / 解放军医学杂志

Fifty cases of primary nephrotic syndrome were studied. There were 40 cases of [type I and 10 cases of type I, all confirmed by renal biopsy. Among them, 17 cases (34%) were mesangial prolife-rativc glomerulonephritis, 12 cases (24%) membranoproliferative glomerulonephritis, 9 cases (18%) proliferative endocapillary glomerulonephritis, 3 cases (6%) membranous nephropathy, 3 cases (6%) minimal change nephrosis, and 6 cases (12%) focal sclerosed glomerulonephritis. 4 to 6-weeks therapy with prednisone, cyclophosphamide and heparin ("triplex therapy") witnessed disappearance of protein-uria in 22 cases, and 44% of the patients showed marked improvement. The total effective rate was 94%. All patients except those with focal sclerosed glomerulonephritis showed favorable response to the treatment. The difference in effectiveness seemed to be related to types and degree of immune injury of the renal pathology.

THE STUDY OF T LYMPHOCYTE SUBSETS AND SUPPRESSOR CELL ACTIVITY IN CHILDREN WITH PRIMARY NEPHRITIC SYNDROME / 西安交通大学学报(医学版)

The peripheral blood T lymphocyte subsets and ConA-induced suppressor cell activity (ConA-SCA) were determined in 37 patients with primary nephritic syndrome(PNS). The results showed that the patients in initial onset had the abnormal number and function of T lymphocytes, which returned to a normal level with treatment with steroid and improvement of patients'condition. The indings suggest that the immunoregulatory imbalance of this disease may be a primary change and play an important role in pathogenesis of PNS.