Clinical application of ultra-micro 5 mm single-port endoscopic thoracic sympathicotomy in selected patients with primary palmar hyperhidrosis / 中国胸心血管外科临床杂志

@#Objective    To assess the feasibility and safety of ultra-micro 5 mm single-port endoscopic thoracic sympathicotomy in selected patients with primary palmar hyperhidrosis. Methods    From March 1, 2018 to February 1, 2021, 90 patients with primary palmar hyperhidrosis who underwent ultra-micro 5 mm single-port endoscopic thoracic sympathicotomy at the Thoracic Surgery Department of the University of Hong Kong-Shenzhen Hospital. There were 47 males and 43 females, with a median age of 26.0 (22.0, 31.0) years. During the operation, T3 and/or T4 thoracic sympathetic nerve chain was transected using an ultra-micro 5 mm single-port incision near the areola or under the axilla. The surgical data of the patients were retrospectively reviewed and analyzed. Results     All patients successfully completed the operation without major bleeding during the operation and no conversion to thoracotomy. There was no death or serious complication during the perioperative period. The operation time was 43.0 (23.0, 60.0) min, and the intraoperative blood loss was 2.0 (1.0, 2.0) mL. In the perioperative period, only one patient needed a tiny chest tube indwelling. The symptoms of hyperhidrosis on the hands all disappeared after the operation. The pain score on the postoperative day was 2.0 (2.0, 2.0) points. The hospital stay after surgery was 1.0 (1.0, 1.0) d. In the first month after the operation, the symptoms of hyperhidrosis on the hands were significantly relieved compared with those before the operation. The surgical incisions healed well, the wounds were concealed, and there was no wound infection or poor healing. The patients' satisfaction with the surgical incisions was 100.0%. After the operation, 14 (15.6%) patients had mild compensatory hyperhidrosis, 5 (5.6%) patients had moderate compensatory hyperhidrosis, and no patient had severe compensatory hyperhidrosis. Overall satisfaction rate was 94.0%. Conclusion     The clinical application of ultra-micro 5 mm single-port endoscopic thoracic sympathicotomy in selected patients with primary palmar hyperhidrosis is safe and feasible. The surgical wound is extremely small and hidden, the operation time is short, the pain is very slight, and the clinical outcome is good. It can fully meet the patients' pursuit of beauty.

Evaluation of therapeutic effects of the ultramicro needle knife combined with cervical spine fine adjusting on youth cervical curvature abnormality case / 中国骨伤

OBJECTIVE@#To study the therapeutic effects of the ultramicro needle-knife combine with cervical spine fine adjusting on youth cervical curvature abnormality case.@*METHODS@#From November 2016 to October 2018, 88 young patients with abnormal curvature of cervical spine were treated. Due to loss of follow up, 86 cases were actually completely including 37 males and 49 females, ranging in age from 20 to 40 years old, with an average of (30.55±5.21) years old, and the course of disease ranged from 1 to 42 months, with a mean of (14.21±7.38) months. All the patients were divided into two groups:treatment group (44 cases) and control group (42 cases). The patients in the treatment group were treated with ultramicro needle-knife and cervical spine fine adjusting, and the patients in the control group were treated with conventional acupuncture and manipulation. The treatments were done 1 time per week in the treatment group while 3 times per week in control group every week, with a duration of 3 weeks for both groups. Before treatment, 3 weeks after treatment, and at the end of 1 month follow-up, the score of neck pain questionnaire(NPQ), range of the motion(ROM) in the cervical region and the D values of cervical physiological curvature were recorded. The efficacy at the end of treatment and in the follow up was evaluated. During the treatment, the patients were also required to correct the bad posture in daily life, to sleep in a low pillow position, and put a moderately columnar pillow behind the neck for 0.5 hours every morning and evening.@*RESULTS@#Two patients in the control group were dropped out after 3 weeks treatment. No adverse reactions were found in the 2 groups during the treatment period. Compared with those before treatment, all scores at all the observation time points were significantly improved between two groups after treatment(all 0.05). There was statistical difference in ROM scores between different time points, there was time effect (=240.32), =0.000). There was no interaction between time factor and group factor (=0.311, =0.734). The effective rate of the treatment group and control group were 90.91%(40 / 44) and 80.95%(34 / 42) respectively, the treatment group was more effective than the control group (0.05) in the follow-up duration.@*CONCLUSION@#The method of needle knife combined with cervical spine fine adjusting has a better therapeutic efficiency than conventional acupuncturecombined with manipulation in treating youth cervical curvature abnormality patients. Because this novel method can recover the cervical curvature, relieve the neck pain, and improve cervical mobility.

Protective Effect of Gastrodiae Rhizoma Powder with Different Particle Sizes on Cerebral Ischemia Reperfusion Injury in Rats / 中国实验方剂学杂志

Objective: To study the protective effect of Gastrodiae Rhizoma ultrafine powder with different particle sizes on focal cerebral ischemia-reperfusion injury in rats, in order to provide theoretical basis for the application of ultrafine powder technology in related products. Method: The SD rats were randomly divided into six groups, namely sham-operated group, ischemia-reperfusion group, Gastrodiae Rhizoma ultramicro-powder Ⅰ group(1.2 g·kg-1), Gastrodiae Rhizoma ultramicro-powder Ⅱ group(1.2 g·kg-1), Gastrodiae Rhizoma ultramicro-powder Ⅲ group(1.2 g·kg-1), and nimodipine positive drug group(12.6 g·kg-1). The cerebral ischemia-reperfusion model was established after 7 days of drug administration. The focal ischemia reperfusion injury model was prepared by middle cerebral artery occlusion (MCAO) for 1 h followed by reperfusion for 24 h. The infarct size of brain tissue was measured by triphenyltetrazolium Chloride(TTC) staining. The activities of superoxide dismutase (SOD), malondialdehyde (MDA) and nitricoxide synthase (NOS) in brain homogenate and serum were measured by colorimetry. The contents of glutamate(Glu), aspartic acid(Asp) and glycine(Gly) in hippocampus were determined by liquid chromatography-tandem mass spectrometry (LC/MSMS). Result:The model group showed obvious signs of neurobehavioral deficit (PPPPPConclusion:Gastrodiae Rhizoma ultramicro-powder with different particle sizes has a protective effect on cerebral ischemia-reperfusion injury, and the Gastrodiae Rhizoma ultramicro-powder Ⅲ group has a more obvious protective effect than the the Gastrodiae Rhizoma ultramicro-powder Ⅰ group. The results showed that the protective effect of Gastrodiae Rhizoma ultramicro-powder on middle cerebral artery ischemia-reperfusion in rats was related to its particle size.

Inhibiting Effect of Ultra-micro Powder Qiweibaizhusan on Bacteria in Vitro / 中国中医药信息杂志

Objective To observe the bacteriostatic effect of single herbs, traditional complex prescription and ultra-micro powder of Qiweibaizhusan. Methods The inhibiting zone and MIC of single herb and compound of Qiweibaizhusan on Escherichia coli, Staphylococcus aureus, Eubacterium aerofaciens, Pseudomonas aeruginosa, Salmonella sp, Saccharomyces cerevisiaes and Candida glabrata were measured by filter paper method. Results The growth of the tested bacteria and yeast except Staphylococcus aureus and Salmonella sp were inhibited by ginseng. The antibacterial effect of licorice was the best, and only Pseudomonas aeruginosa’s growth was not inhibited by licorice. The growth of Staphylococcus aureus and Eubacterium aerofaciens were inhibited by Agastache rugosa. The growth of Escherichia coli, Staphylococcus aureus, Eubacterium aerofaciens and Pseudomonas aeruginosa were inhibited by Poria cocos. Only Eubacterium aerofaciens’s growth was inhibited by Radix aucklandiae and fried Atractylodes macrocephala. The growth of all the bacteria and yeast were not inhibited by Radix puerariae. The growth of Staphylococcus aureus, Eubacterium aerofaciens and Salmonella sp were inhibited by the traditional decoction and ultra-micro powder of complex prescription of Qiweibaizhusan, and all the MIC of ultra-micro powder were smaller than the traditional decoction. Conclusion The main antibacterial component of Qiweibaizhusan was ginseng and licorice. The inhibiting effect of ultra-micro powder on bacteria was better than traditional decoction of Qiweibaizhusan in vitro.

Content Determination of Three Ginsenosides in Shengmai Ultra-micro Powder / 世界科学技术-中医药现代化

This study was aimed to establish an HPLC method to determine three ginsenosides in Shengmai ultra-micro powder. The kromasil C18 (250 mm í 4.6 mm, 5 μm) was used as analytical column. The mobile phase was composed of acetonitrile (A) and water (B) with gradient elution (0~35 min, 19% A; 35~55 min, 19%~29% A; 55~70 min, 29% A; 70~100 min, 29%~40% A) at a flow rate of 1 mL·min-1. The detection wavelength was 203 nm and the column temperature was 30℃. The injection volume was 10 μL. The results showed that the linear ranges of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 were 0.083~0.834 μg, 0.086~0.863 μg, 0.091~0.911 μg, respec-tively. The average recovery rates (n = 6) were 100.7%, 100.5%, 100.5%, respectively. It was concluded that this method was quick, sensitive, repeatable and suitable to determine contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Shengmai ultra-micro powder.

Prevalencia de hepatitis B en donantes de sangre normal y específicos en el Banco de Sangre Provincial de Matanzas / Prevalence of the hepatitis B in normal and specific blood donors in the provincial bank of Matanzas

Se realizó un estudio descriptivo transversal en donantes de sangre normal y de plasma especifico del Banco de Sangre Provincial, de Matanzas, con previa inmunización o no mediante la vacuna Herbiovac durante el período comprendido entre enero de 2009 y diciembre de 2010, con el propósito de analizar la prevalencia de la positividad de hepatitis B mediante el pesquisaje de marcadores serológicos, diferenciar la positividad en los donantes de sangre normal y de plasma específico, e identificar el comportamiento de variables como la edad y el sexo de estos pacientes reactivos al antígeno de superficie. Se obtuvieron los siguientes resultados: disminución de la prevalencia de positivos en el 2010 con relación al 2009; no se encontró muestras reactivas en las plasmaféresis. El 71,7 % de los casos positivos perteneció al sexo masculino, el rango de mayor edad estuvo entre los 36-45 años con 61,5 % de reactividad. Este estudio permitió afirmar que en el territorio de atención del Banco de Sangre Provincial existe una prevalencia baja de reactividad al antígeno de superficie en donantes de sangre, al igual que los resultados nacionales. La aplicación de una buena selección del donante y el uso de la consejería predonación, favorecen la disminución de la positividad en donantes de sangre. Los resultados obtenidos para la plasmaféresis muestran la efectividad de la aplicación de la vacuna para inmunizar contra la hepatitis B.

We carried out a crossed, descriptive research in normal blood and plasma specific donors of the Provincial Blood Bank of Matanzas with previous immunization or not by means of the Herbiovac vaccine during the period between January 2009 and December 2010. Our purpose was analyzing the prevalence of hepatitis B positivity through serologic markers screening, differentiating the positivity in the normal blood and plasma specific donors and identifying the behavior of variables like age and genre of the patients that are reactive to the surface antigen. We obtained the following results: the prevalence of positives decreased in 2010 in relation with 2009; no reactive samples were found in the plasmapheresis; 71,7 % of the positive cases were male; the eldest age group was the 36-45 years-old one with 1,5 % of reactivity. This research allows us to affirm that there is a low prevalence of reactivity to the surface antigen among the blood donors of the territory covered by the Provincial Blood Bank of Matanzas, like the results at the national level. Applying a good selection of the donors and using the pre-donation counseling favor the decrease of the positivity in blood donors. The results obtained for the plasmapheresis show the vaccine effectiveness to reach the immunization against the hepatitis B.

Experimental Study on Ultramicro-powder Dosage of Chinese Complex Prescription / 国际中医中药杂志

The ultramicro-powder dosage is a new type decoction pieces of Chinese medicine.Due to high cell-wall penetrating rate of vegetal cell and increasing of specific surface area,the development of ultramicro-powder dosage has great significance to increasing clinical effects and bioavailability of Chinese medicine.

Study on effect of ultramicro-shatter technology on penetrating skin absorption of Pollen Typhae in Zhongtongxiao Cataplasm / 中华中医药杂志

Objective:To study the effect of ultramicro-shatter technology on penetrating skin absorption of isorhamnetin-3-O-neohesperidin in Zhongtongxiao Cataplasm.Methods:To apply reformed Frans penetrating skin absorption cell marching extraorgan penetrating skin experiment.HPLC method was used to determine the content of isorhamnetin-3-Oneohesperidin in ultramicro-shatter Zhongtongxiao Cataplasm and in common Zhongtongxiao Cataplasm.Results:The Q-t equation of ultramicro-shatter Zhongtongxiao Cataplasm:Q=3.0382t+47.082,penetrating skin velocity:3.0382(?g.cm2/h);the Q-t equation of common Zhongtongxiao Cataplasm:Q=2.7967t+39.752,penetrating skin velocity:2.7967(?g.cm2/h);Extraction rate of dynamic extracting micro-powder,the ephedrina hydrochloridum,glycyrrhizic acid and glycyrrhizae glycoside were higher than the trdtional cut crude drug decocting.Conclusion:The accumulating osmolality and penetrating skin velocity of isorhamnetin-3-O-neohesperidin in ultramicro-shatter Zhongtongxiao Cataplasm were all better than those in common Zhongtongxiao Cataplasm,it explained that ultramicro-shatter technology accelerate the dissolution of medicine compsitions.

Study on pharmacokinetics of brucine from normal and ultra-micro powder of semen strychni in rats / 中华中医药杂志

Objective:To study the pharmacokinetic process ofbrucine from normal and ultra-micro powder ofsemen strychni in rat serum.Methods:HPLC techniques were used.All pharmacokinetics parameters were calculated with a 3P87 program.Results:The pharmacokinetics results indicated that the normal powder and the ultra-micro powder ofsemen strychni belonged to two compartment open model.The absorb ofbrucine was just several ten minutes, and there was no obvious difference ofTmax between normal powder and ultra-micro powder.This showed that:Brucine was some material that was easily absorbed.But we can find that Cmax increased from(51.13?0.48)?g/L to(69.26?1.40)?g/L after ultra-micro powder preparation which was quite obvious.K,Ka,B and ? had a obvious increase(P

Effect of ultra-micro Guipiwan on model rat with both qi and blood def iciency / 中华中医药杂志

Objective:To compare the effect of ordinary Guipiwan and Ultra-micro Guipiwan on the rat models of both qi and blood defi ciency.Methods:Rat models of both qi and blood defi ciency,induced by re1easing blood and intraperitoneal injections of cyclophosphamide,were divided into model group,high,middle,and low dosage ultra-micro Guipiwan solution groups,ordinary Guipiwan solution group and blank control group.Drugs were given once a day intragastrically,successively for 14 days.At the end of the experiment,the countings of red cells,white blood cells,platelet,hemoglobin and the levels of granulocyte-macrophage colony stimulating factor in serum and were measured.Results:Ultra-micro Guipiwan increased the levels of blood cells,platelet,hemoglobin and the levels of granulocyte-macrophage colony stimulating factor in serum of the rats with both qi and blood defi ciency,the effect was better than Guipiwan(P

Comparative study on dissolution of strychnine in normal and ultra-micro powder of semen strychi / 中华中医药杂志

Objective:Dissolution test was applied to study the dissolution rates of strychnine from the normal and ultramicro powder of semen strychi. Methods:HPLC techniques was used to detect the dissolution amount and the dissolution velocity of strychnine from the normal and ultra-micro powder of semen strychi. Results:The dissolution velocity of strychnine from the ultra-micro powder was higher than that from the normal powder,but there was no difference in the dissolution amount 4 hours later. Conclusion:The dissolution velocity of strychnine from the ultra-micro powder increased greatly and there was no loss of the principal active.

Study on the GC fingerprints of volatile oil in ultramicro-powder of Houttuynia cordata / 中华中医药杂志

Objective:To establish a GC fingerprint analysis method for identification of volatile oil in ultramicro-powder of Houttuynia cordata from different habitats, then to control the quality sensitively.Methods: GC was used to analyze the volatile constituents of ultramicro-powder of Houttuynia cordata from 12 different habitats;SPDTM-1CapillaryColumn(30m?0.32mm?0.25?m),temperature programming and FID detector were applied.Results: The mutual mode of GC fingerprints was set up and the similar degrees to the volatile oil from of different habitats were compared.Conclusion:The GC fingerprints of volatile oil in ultramicro-powder can be used to identify the Houttuynia cordata from different habitats and evaluate its quality.

Effect on the micro- and ultramicro-structure of intrahepatic bile duct mediated by hypoxic preconditioning after liver graft / 中国现代普通外科进展

Objective: To investigate the change of the micro- and ultramicro-structure of in- trahepatic bile duct after liver graft and the protection of hypoxic preconditioning. Methods: The model of orthotopic autologous liver transplantation was used, thirty SD rats were randomly divided into three groups. A: orthotopic autologous liver transplantation group; B: hypoxic preconditioning before operation group; C: sham operation group. The serum bilirubin ,the micro-structure of biliary epithelial cell and the ultramicro-structure of cholangiole were determined in three groups after 48hours after operation. Results: As compared with B group: the serum bilirubin increased (P

Parallel ultramicro and macro determinations of blood sugar

A modified ultramicro method for blood sugar determination, using locally available apparatus has been described. This method gives very close results to the macro method of Folin and Wu. The difference being insignificant, it may be concluded that the ultramicro method is just as good, if not better than the macro method of Folin and Wu. (Conclusion)

Protective Action of Ultramicro-powder of Rhizoma Gastrodiae on Nerves in the Rat of Cerebral Ischemia-Reperfusion Injury / 中医杂志

Objective:To explore protective action of ultramicro-powder of Rhizoma Gastrodiae on nerves in the rat of Cerebral ischemia- reperfusion injury.Methods:The local ischemia-reperfusion injury at model was established by cerebral ischemia for 2 hours and reperfusion for 24 hours.HE staining,2,3,5-triphenytetrazolium(TTC)staining,immunohistochemical methods were respectively used to investigate pathological changes of brain slices,the size of cerebral infarction and nervous cell apoptotic relative proteins Bcl-2,Bax expression after treatment with ultramicro-powder of Rhizoma Gastrodiae.Results:(1)Ultramicro-powder of Rhizoma Gastrodiae could reduce the size of cerebral infarction.When the low,middle and high dose groups were compared with the ischemia group there were significant differences(P

HPLC fingerprint of ultramicro decoction piece of Radix paeomae Alba / 中成药

AIM: To determine the HPLC fingerprint of ultramicro decoction piece of Radix paeoniae Alba. METHODS: HPLC was used to analyze the extracts of ultramicro decoction piece of Radix paeomae Alba from 10 different sources. RESULTS: The fingerprint of ultramicro decoction piece of Radix paeoniae Alba was composed of 20 peaks,among which there were 10 characteristic peaks. CONCLUSION: The fingerprmt can be used to control the ultramicro decoction piece of Radix paeoniae Alba qualities.

HPLC fingerprint of ultramicro decoction piece of Fructus gardeniae / 中成药

AIM: To determine the HPLC fingerprint of ultramicro decoction piece of Fructus gardeniae. METHODS: Chromatographic conditions were as follows: column: Hypersil BDS C_ 18 (4.6 mm?200 mm,5 ?m), mobile phase: acetonitrile-water (10∶90), column tempemture: 25 ℃,flow rate: 1 mL?min -1 , wavelength: 238 nm, and inject volume: 10 ?L. RESULTS: The fingerprint of ultramicro Fructus gardeniae was established and 7 common peaks in the fingerprint were indicated. CONCLUSION: It can nearly completely reflect intrinsic quality of ultramicro decoction piece of Fructus gardenian.

Comparison of dissolution of asperosaponin Ⅵ between ultra-micro powder and fine powder of Radix Dipsaci / 中成药

AIM:To compare the dissolution of asperosaponin Ⅵ between the ultra-micro powder and the fine powder of Radix Dipsaci.METHODS:The real contents,in vitro release and releasing rate of asperosaponin Ⅵwere determined by HPLC for the ultra-micro powder and the fine powder.RESULTS:In the ultra-micro powder and the ordinary powder,the real content of asperosaponin Ⅵ were 4.87%,4.74%,respectively;in vitro release in 1 h were 48.2 mg/g,47.5 mg/g,respectively;releasing rate parameter T_ 0.9 were 0.23 min,10.41 min,respectively.CONCLUSION:The ultra-micro porphyrization could not influent the real content and in vitro release of asperosaponin Ⅵ in Radix Dipsaci.But it could improve the releasing rate of asperosaponin Ⅵ.

EFFECT OF ZINC DEFICIENCY ON MACROPHAGE AND MUCOSAL EPITHELIUM OF SMALL INTESTINE IN MICE / 营养学报

Zinc deficient model was made by feeding zinc deficient rations to mice. Their general appearance, body weight, organ body weight ratio, macroph-age (M?) and intestinal mucosal epithelium were observed. Rations intake of these Zn deficient mice decreased 2 weeks later. There was prominent difference in body weight between the Zn deficient (n = 24) and the control group (n =11), and the difference was more prominent 4 weeks later. The mortality of the mice in Zn dificient group was 29.17% while all of the control mice were alive. Weight of liver, spleen, kidney, adrenal glands thymus and the oryan body weight ratio of the thymus of Zn deficient mice decreased markedly. Under the scanning electron microscope, the following variance were shown. The superficial structure of M? in the Zn deficient group tended to be simplified, the pseudopodium tended to be decreased and short. The ratio of activated cells to total M?cells decreased to 56.4% while that of the control group was 81.9% (P