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Adv Rheumatol ; 61: 16, 2021. tab, graf
Article in English | LILACS | ID: biblio-1152746


Abstract Backgrounds: It is important to improve our understanding of the roles of calcium and vitamin D in bone health for preventing osteoporosis. We aimed at exploring the associations between serum calcium, vitamin D level, and bone mineral density (BMD) in adolescents included in the National Health and Nutrition Examination Survey (NHANES) 2001 - 2006. Methods: Weighted multivariate linear regression models were used to estimate the associations of serum calcium, 25(OH)D level with total BMD. Smooth curve fitting was used to explore the potential non-linear relationship. Results: A total of 5990 individuals aged between 12 and 19 years were included in this study. The fully-adjusted model showed serum calcium positively correlated with total BMD. However, an inverted U-shaped relationship was found when we performed the smooth curve fitting method, and the inflection point was calculated at 9.6 mg/dL using the two-piecewise linear regression model. In contrast, there was a positive correlation between serum 25(OH)D and total BMD after adjusting for potential confounders. Conclusions: The present study revealed a positive correlation between serum 25(OH)D level and total BMD, and an inverted U-shaped relationship between serum calcium and total BMD.(AU)

Humans , Adolescent , Vitamin D/analysis , Bone Density , Calcium/analysis , Linear Models , Nutrition Surveys , Multivariate Analysis
Medicina (B.Aires) ; 80(1): 87-90, feb. 2020. ilus
Article in Spanish | LILACS | ID: biblio-1125042


El síndrome de Gitelman forma parte de las denominadas tubulopatías perdedoras de sal. El bloqueo parcial de la reabsorción de sodio en el túbulo contorneado distal determina la aparición de hipokalemia e hipomagnesemia. Se realizó un estudio descriptivo de una serie de cinco casos de síndrome de Gitelman (4 mujeres, de 28 a 85 años de edad) atendidos en nuestra institución entre los años 2004 y 2015. La forma de diagnóstico más frecuente en nuestra serie fue por hallazgo de laboratorio. El único síntoma clínico manifestado en forma espontánea fue astenia. En cuanto a los valores de laboratorio, la potasemia fue 2.5 ± 0.5 mmol/l, con un valor mínimo de 2.1. Adicionalmente, el valor de magnesio en sangre fue 1.3 ± 0.3 mg/dl. Como conclusión, observamos que las formas de presentación consisten en alteraciones bioquímicas con o sin manifestaciones inespecíficas, lo que representa actualmente la mayor dificultad diagnóstica y refuerza la importancia de lograr un diagnóstico oportuno, en especial en pacientes jóvenes y con valores críticos de potasio sérico.

Gitelman syndrome is one of the salt losing tubulopathies. Hypokalemia and hypomagnesemia appear in the setting of the partial blockade of salt absorption in the distal tubule. We conducted a descriptive study of a case series of five patients with Gitelman syndrome (4 women, from 28 to 85 years) in our institution, between the years 2004 and 2015. The most frequent form of diagnosis in our series was by laboratory finding. The only acknowledged clinical symptom was malaise. Regarding laboratory findings, the mean potassemia was of 2.5 ± 0.5 mmol/l, with a minimum value of 2.1 mmol/l. Additionally, the serum magnesium value was of 1.3 ± 0.3 mg/dl. In conclusion, we observed that the forms of presentation consist of biochemical alterations with or without nonspecific manifestations, which currently represents the greatest diagnostic difficulty and reinforces the importance to achieve a timely diagnosis, especially in young patients with critical serum potassium values.

Humans , Male , Female , Adult , Middle Aged , Aged, 80 and over , Gitelman Syndrome/diagnosis , Gitelman Syndrome/therapy , Potassium/analysis , Asthenia/diagnosis , Calcium/analysis , Gitelman Syndrome/metabolism , Magnesium/analysis
Braz. j. med. biol. res ; 53(3): e8761, 2020. tab, graf
Article in English | LILACS | ID: biblio-1089339


Nitric oxide (NO) inhibition by high-dose NG-nitro-L-arginine methyl ester (L-NAME) is associated with several detrimental effects on the cardiovascular system. However, low-dose L-NAME increases NO synthesis, which in turn induces physiological cardiovascular benefits, probably by activating a protective negative feedback mechanism. Aerobic exercise, likewise, improves several cardiovascular functions in healthy hearts, but its effects are not known when chronically associated with low-dose L-NAME. Thus, we tested whether the association between low-dose L-NAME administration and chronic aerobic exercise promotes beneficial effects to the cardiovascular system, evaluating the cardiac remodeling process. Male Wistar rats were randomly assigned to control (C), L-NAME (L), chronic aerobic exercise (Ex), and chronic aerobic exercise associated to L-NAME (ExL). Aerobic training was performed with progressive intensity for 12 weeks; L-NAME (1.5 mg·kg-1·day-1) was administered by orogastric gavage. Low-dose L-NAME alone did not change systolic blood pressure (SBP), but ExL significantly increased SBP at week 8 with normalization after 12 weeks. Furthermore, ExL promoted the elevation of left ventricle (LV) end-diastolic pressure without the presence of cardiac hypertrophy and fibrosis. Time to 50% shortening and relaxation were reduced in ExL, suggesting a cardiomyocyte contractile improvement. In addition, the time to 50% Ca2+ peak was increased without alterations in Ca2+ amplitude and time to 50% Ca2+ decay. In conclusion, the association of chronic aerobic exercise and low-dose L-NAME prevented cardiac pathological remodeling and induced cardiomyocyte contractile function improvement; however, it did not alter myocyte affinity and sensitivity to intracellular Ca2+ handling.

Animals , Male , Physical Conditioning, Animal/physiology , Calcium/analysis , Nitric Oxide Synthase/drug effects , NG-Nitroarginine Methyl Ester/pharmacology , Enzyme Inhibitors/pharmacology , Myocardial Contraction/drug effects , Body Weight/physiology , Rats, Wistar , Ventricular Pressure/drug effects , Nitric Oxide Synthase/metabolism , NG-Nitroarginine Methyl Ester/administration & dosage , Models, Animal , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/physiology , Enzyme Inhibitors/administration & dosage , Adiposity , Hemodynamics , Motor Activity/physiology , Myocardium/pathology
Braz. arch. biol. technol ; 63: e20180670, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132160


Abstract Studies evaluating the influence of nutrients on plant anatomy are very important because nutritional deficiencies can alter the thickness and shape of certain tissues, compromising their functionality what can explain the reduction of productivity. The aim of this study was to characterize the anatomical changes in cherry tomato plants subjected to calcium (Ca) and magnesium (Mg) deficiencies. The experiment was conducted in nutrient solution and the plants subjected to three treatments: complete solution (Control), Ca restriction (1 mmol L-1 of Ca) and Mg omission (without Mg). The experimental design was completely randomized, with three repetitions. Sixty days after seedling transplanting leaves and stem were collected and submitted to anatomical evaluations. Ca or Mg deficiency promotes most evident anatomical changes in chlorophyllous and vascular tissues of the leaves, rather than in the stems. Leaves of 'Sindy' tomato plants with a concentration of 1.7 g kg-1 of Mg and visual symptoms of Mg deficiency present hyperplasia of both tissues, phloem and xylem. This deficiency also promotes increases in the thickness of mesophyll, spongy parenchyma and palisade parenchyma, and consequently of leaf thickness. The midrib of the leaves with a concentration of 10 g kg-1 of Ca, without visual symptoms of deficiency presented phloem hypertrophy and hyperplasia.

Calcium/analysis , Lycopersicon esculentum/chemistry , Plant Stems/chemistry , Plant Leaves/chemistry , Magnesium/analysis , Phloem/metabolism , Xylem/metabolism
Actual. osteol ; 15(3): 205-213, Sept-Dic. 2019. ilus, tab
Article in English | LILACS | ID: biblio-1104336


The dental caries is a progressive destruction of the teeth tissue due to the disbalance in the normal molecule interactions between the enamel and the bio!lm, which alters the demineralization-remineralization process. Milk fermentation produces caseinphosphopeptides with proved remineralizing capacity of the enamel. The presence of these peptides in fermented milk with ke!r grains has been described. The purpose of this work was to evaluate in vitro the capacity of milk ke!r to prevent the demineralization of dental enamel. Bovine incisors (n=68, 17 per group) were treated for 72 h with different solutions: I: artificial saliva at pH 7.2 , II: demineralizing solution at pH 4.5, III: supernatant of kefir fermented milk at pH 4.5, IV: milk supernatant at pH 4.5. The effects of treatments were evaluated by the change in the weight of the specimens, calcium concentration in the solution and by scanning electron microscopy (SEM) of the enamel. Kefir milk supernatant prevented the demineralization process, that was evidenced by a change in weight and calcium concentration that were not different from group I, although the pH was 4.5. In contrast, group IV showed a decrease in weight and an increase in calcium concentration, compared with group I (one way ANOVA, p<0.05). Images of SEM agree with the values of weight and calcium concentration. These results indicate that kefir milk supernatant has a protective effect on enamel demineralization in vitro. (AU)

La caries dental es una patología debido a un desequilibrio en las interacciones moleculares normales entre el esmalte y la biopelícula, que altera el proceso de desmineralización remineralización. La fermentación de la leche produce fosfopéptidos de caseína con probada capacidad remineralizante del esmalte, y se ha descripto la presencia de estos péptidos en la leche fermentada con granos de kéfir. El propósito de este trabajo fue evaluar in vitro la capacidad del kéfir de leche para prevenir la desmineralización del esmalte dental. Sesenta y ocho incisivos bovinos (17 por grupo) fueron tratados durante 72 h con diferentes soluciones: I: saliva artificial, pH 7.2, II: solución desmineralizante, pH 4.5, III: sobrenadante de leche fermentada con kefir, pH 4.5, IV: sobrenadante de leche, pH 4.5. El proceso de desmineralización se evaluó mediante el cambio en el peso de las muestras, la concentración de calcio en la solución y microscopía electrónica de barrido (SEM) del esmalte. El sobrenadante de leche fermentada con kéfir impidió el proceso de desmineralización, que se evidenció por un cambio en el peso y la concentración de calcio que no discreparon del grupo I, a pesar de haber tenido un pH de 4.5. En contraste, el grupo IV mostró una disminución en el peso y un aumento en la concentración de calcio, en comparación con el grupo I (ANOVA a un criterio, p<0.05). Las imágenes SEM concuerdan con los cambios en el peso y la concentración de calcio en los grupos estudiados. Los datos obtenidos demuestran que el sobrenadante de la leche tratada con kéfir tiene un efecto protector sobre la desmineralización del esmalte in vitro, inducida por el pH ácido. (AU)

Animals , Cattle , Tooth Demineralization/prevention & control , Kefir/microbiology , Saliva, Artificial/administration & dosage , Tooth Remineralization/methods , In Vitro Techniques , Cattle , Caseins/therapeutic use , Calcium/analysis , Tooth Demineralization/pathology , Tooth Demineralization/therapy , Biofilms , Dental Caries/prevention & control , Dental Enamel/cytology , Dental Enamel/physiopathology , Milk/microbiology , Formaldehyde/administration & dosage
Int. braz. j. urol ; 45(6): 1249-1259, Nov.-Dec. 2019. tab, graf
Article in English | LILACS | ID: biblio-1056334


ABSTRACT Objective: Urinary stones with oxalate composition can cause kidney failure. Recent findings evidenced that probiotics are effective in reducing oxalate absorption in these subjects based on their high colonic absorption levels at baseline. The purpose of this study was to evaluate the effect of the simultaneous use of oxalate-degrading bacteria, Urtica dioica and T. terrestris extract in reducing urinary oxalate. Materials and Methods: Anti-urolithiatic activity of Urtica dioica and T. terrestris extract and probiotic by using ethylene glycol induced rat model. In this study, 4 strains of Lactobacillus and 2 strains of Bifidobacterium and also 2 strains of L. paracasei (that showed high power in oxalate degrading in culture media) were used. Male Wistar rats were divided into four groups (n=6). The rats of group-I received normal diet (positive control group) and groups-II (negative control group), III, IV rats received diet containing ethylene glycol (3%) for 30 days. Groups III rats received Urtica dioica and T. terrestris extract. Groups IV rats received extracts + probiotic for 30 days. Findings: The results show that the use of herbal extracts (Urtica dioica and T. terrestris) reduced the level of urinary oxalate and other parameters of urine and serum. Also, the accumulation of calcium oxalate crystals in the kidney tissue was significantly reduced. Conclusion: Considering that the formation of calcium oxalate crystals can cause inflammation and tissue damage in the kidney, the use of herbal extracts with oxalate degrading bacteria can be a new therapeutic approach to preventing the formation of kidney stones.

Animals , Male , Oxalates/urine , Hyperoxaluria/prevention & control , Plant Extracts/pharmacology , Probiotics/pharmacology , Urtica dioica/chemistry , Tribulus/chemistry , Reference Values , Time Factors , Blood Urea Nitrogen , Kidney Calculi/urine , Kidney Calculi/prevention & control , Calcium/analysis , Reproducibility of Results , Rats, Wistar , Creatinine/analysis , Kidney Tubules/chemistry
São Paulo; s.n; s.n; 2019. 77 p. tab, graf.
Thesis in English | LILACS | ID: biblio-1007424


Mitochondria are central players in cell metabolism, responsible for the vast majority of ATP production in most cells. Although originally thought to be passive organelles focused only in keeping cellular ATP at adequate levels, complex interplay between mitochondrial function and cell signaling has been largely recognized over the last decades. Not surprisingly, given their role, changes in nutritional status promoted by chronic interventions like caloric restriction or short-term situations like fasting in animals or nutrient deprivation in cultured cells are one of the main factors that can activate those signaling mechanisms. One particular way in which this mitochondria-cell crosstalk can occur is through mitochondrial Ca2+ handling, a process in which Ca2+ signals generated by the cell are able to translate into elevations in mitochondrial matrix [Ca2+] due to the presence of the mitochondrial Ca2+ uniporter in the organelle. While the impact of mitochondrial Ca2+ handling on cellular function has been widely studied, the conditions which can modulate the process of mitochondrial Ca2+ handling itself are still not well characterized. In this work, we sought to test the effects of different interventions linked to nutritional status on mitochondrial Ca2+ handling. We found that caloric restriction, physiological fasting and modulations of mitochondrial dynamics resulted in modulation of mitochondrial Ca2+ handling through changes in their maximal Ca2+ retention capacity or Ca2+ uptake rates. These changes were, measured by following mitochondrial Ca2+ uptake using different strategies, employing the fluorescent Ca2+ probe Ca2+ Green 5N for experiments in isolated mitochondria and permeabilized cells and the cytosolic probe Fura2-AM in intact cells. Caloric restriction resulted in higher calcium uptake and retention in liver mitochondria, protecting against pathological conditions of Ca2+ overload during ischemia/reperfusion. On the other hand, overnight and short term fasting resulted in lower mitochondrial Ca2+ retention and oxidative phosphorylation capacity in the liver. Modulating mitochondrial morpholoy in C2C12 myoblasts showed that more fragmented mitochondria were less capable of taking up Ca2+, while more fusioned mitochondria showed the opposite phenotype. This modulation in Ca2+ handling through changes in mitochondrial morphology interfered with the process of Store-Operated Ca2+ entry in the cells, showing that these modulations can have impacts in physiological contexts as well. Overall, this work both establishes novel mechanisms of modulation of mitochondrial Ca2+ handling and demonstrates their relevance both in pathology and normal cellular physiology

Mitocôndrias possuem um papel central no metabolismo das células, sendo responsáveis pela maioria da produção de ATP na maioria dos tipos celulares. Embora originalmente se pensasse nas mitocôndrias como organelas estáticas, focadas somente em manter os níveis adequados de ATP na célula, a interação entre a função mitocondrial e a sinalização celular tem sido fortemente reconhecida nas ultimas décadas. Dado este papel, não é surpreendente que mudanças no estado nutricional, tanto crônicas como na restrição calórica quanto em situações como o jejum em animais e a privação de nutrientes em cultura de células foram demonstradas como sendo um dos principais fatores que podem ativar estes mecanismos de sinalização. Uma das formas em que esta interação entre a mitocôndria e a célula ocorre é através do manejo de Ca2+ mitocondrial, um processo em que sinais de Ca2+ gerados pela célula podem resultar em aumentos na [Ca2+] na matriz mitocondrial devido à presença do uniportador de Ca2+ mitocondrial na organelaEmbora o impacto do manejo de Ca2+ mitocondrial na função da célula tenha sido amplamente estudado, a regulação do processo de manejo de Ca2+ mitocondrial em si não é bem conhecida. Neste trabalho, nós nos propusemos a testar os efeitos de diferentes intervenções ligadas ao estado nutricional no manejo de Ca2+ mitocondrial e o possível impacto destas modulações nacapacidade de retenção e na taxa de captação de Ca2+ mitochondrial. As intervenções estudadas foram a restrição calórica, jejum e mudanças na dinâmica mitocondrial, e todas elas resultando em mudanças no manejo de Ca2+ mitocondrial, que foram medidos acompanhando a captação de Ca2+ em mitocôndrias isoladas ou células permeabilizadas utilizando a sonda Ca2+ Green 5N e em células intactas utilizando a sonda de Ca2+ citosólica Fura2-AM. Enquanto a restrição calórica resultou em uma maior capacidade de retenção de Ca2+ e em maiores taxas de captação, protegendo contra as condições patológicas de desregulação de Ca2+ observadas durante a isquemia/reperfusão, o jejum curto ou pela duração da noite resultou em uma diminuição na capacidade de retenção de Ca2+ e na oxidação fosforilativa mitocondriais. As mudanças observadas modulando a dinâmica mitocôndria (feitas utilizando-se mioblastos da linhagem C2C12) revelaram que mitocôndrias mais fragmentadas são menos capazes de captar Ca2+, enquanto mitocôndrias mais fusionadas possuem o fenótipo oposto. Essas mudanças no manejo de Ca2+ mitocondrial interferem com o processo de Store-Operated Ca2+ entry nestas células, demonstrando que essas modulações da captação de Ca2+ mitocondrial também podem ser relevantes em contextos fisiológicos. Em resumo, este trabalho ajudou a estabelecer novos mecanismos de modulação do manejo de Ca2+ mitocondrial que podem ser relevantes tanto em condições patológicas quanto na fisiologia normal das células

Calcium/analysis , Nutritional Status , Mitochondrial Dynamics , Cell Death/immunology , Myoblasts/classification , Mitochondria/chemistry
J. appl. oral sci ; 27: e20180188, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-984574


Abstract Sources of calcium and phosphate have been added to dental restorative materials to improve their anticaries effect. Objective This study evaluated the effect of adding calcium glycerophosphate (CaGP) to resin-modified glass ionomer cement (RMGIC) on the physico-mechanical properties, ion release, and enamel demineralization. Material and Methods: Specimens were fabricated for each experimental group: RMGIC without CaGP (Control), RMGIC with 1, 3 and 9% CaGP. To determine the release of fluoride (F), calcium (Ca) and phosphorus (P), six specimens were immersed in demineralization and remineralization solutions for 15 days. In another experimental trial, the following physico-mechanical properties were evaluated at time intervals of 1 and 7 days after fabrication: compressive strength (n=12), diametral tensile strength (n=12), surface hardness of material (n=6) and the degree of conversion of monomers (n=8). To study enamel demineralization, specimens (n=12) were attached to enamel blocks and submitted to pH-cycling. Subsequently, surface and cross-sectional hardness and the concentration of F, Ca and P in enamel were determined. Results The addition of CaGP to RMGIC led to higher mean release of F, Ca and P when compared with control (p<0.001). Mechanical properties were within the range of those of the ionomer cements after addition of 1% and 3% CaGP. The degree of conversion did not differ between groups at the 1st and the 7th day (p>0.439). The addition of 3% and 9% CaGP reduced mineral loss and increased F, Ca and P in the enamel when compared with control (p<0.05). Conclusion The addition of 3% CaGP in RMGIC increased the release of F, P and Ca, reduced enamel demineralization, and maintained the physico-mechanical properties within the parameters for this material.

Humans , Tooth Demineralization/prevention & control , Resin Cements/chemistry , Dental Enamel/drug effects , Glass Ionomer Cements/chemistry , Glycerophosphates/chemistry , Phosphates/analysis , Reference Values , Surface Properties , Time Factors , Materials Testing , Photomicrography , Calcium/analysis , Reproducibility of Results , Compressive Strength , Dental Enamel/chemistry , Fluorides/analysis , Hardness Tests
Arq. bras. cardiol ; 111(3): 400-409, Sept. 2018. tab, graf
Article in English | LILACS | ID: biblio-973754


Abstract Background: Caloric restriction is known to impair the cardiac function and morphology in hypertrophied hearts of spontaneously hypertensive rats (SHR); however, the influence of fasting/refeeding (RF) is unknown. Objective: To investigate the fasting/refeeding approach on myocardial remodeling and function. In addition, the current study was designed to bring information regarding the mechanisms underlying the participation of Ca2+ handling and b-adrenergic system. Methods: Sixty-day-old male SHR rats were submitted to food ad libitum (C), 50% food restriction (R50) or RF cycles for 90 days. Cardiac remodeling was assessed by ultrastructure analysis and isolated papillary muscle function. The level of significance considered was 5% (a = 0.05). Results: The RF rats presented lower cardiac atrophy than R50 in relation to C rats. The C rats increased weight gain, R50 maintained their initial body weight and RF rats increased and decreased weight during RF. The RF did not cause functional impairment because the isotonic and isometric parameters showed similar behavior to those of C. The isotonic and isometric cardiac parameters were significantly elevated in RF rats compared to R50 rats. In addition, the R50 rats had cardiac damage in relation to C for isotonic and isometric variables. While the R50 rats showed focal changes in many muscle fibers, the RF rats displayed mild alterations, such as loss or disorganization of myofibrils. Conclusion: Fasting/refeeding promotes cardiac beneficial effects and attenuates myocardial injury caused by caloric restriction in SHR rats, contributing to reduce the cardiovascular risk profile and morphological injuries. Furthermore, RF promotes mild improvement in Ca2+ handling and b-adrenergic system.

Resumo Fundamento: A restrição calórica compromete a função e a morfologia cardíacas em corações hipertrofiados de ratos espontaneamente hipertensos (SHR). No entanto, a influência de ciclo de jejum/Realimentação é desconhecida. Objetivo: Investigar o efeito de ciclos de jejum/realimentação sobre a remodelação e função miocárdica. Além disso, o presente estudo foi desenhado para avaliar os mecanismos subjacentes à participação do trânsito de cálcio (Ca+2) e sistema beta-adrenérgico. Métodos: Neste estudo, SHR machos de 60 dias de idade foram submetidos a alimento ad libitum (grupo C), 50% de restrição alimentar (grupo R50) ou ciclos de RF (grupo RF) por 90 dias. A remodelação cardíaca foi avaliada por meio da análise ultraestrutural e função do músculo papilar isolado. Adotou-se o nível de significância de 5% (a = 0,05). Resultados: Os ratos do grupo RF apresentaram menor atrofia cardíaca do que os do grupo R50 em relação aos do grupo C. Os ratos do grupo C aumentaram peso corporal, os ratos do grupo R50 mantiveram seu peso corporal inicial e os ratos do grupo RF aumentaram e reduziram seu peso durante o ciclo RF. O ciclo RF não causou comprometimento funcional, pois os parâmetros isotônicos e isométricos apresentaram comportamento similar aos dos ratos do grupo C. Os parâmetros cardíacos isotônicos e isométricos mostraram-se significativamente elevados nos ratos do grupo RF em comparação aos dos ratos do grupo R50. Além disso, os ratos do grupo R50 apresentaram dano cardíaco em comparação aos ratos do grupo C quanto às variáveis isotônicas e isométricas. Os ratos do grupo R50 apresentaram alterações focais em muitas fibras musculares, enquanto os ratos do grupo RF apresentaram leves alterações, como perda ou desorganização de miofibrilas. Conclusão: Ciclos de Jejum/Realimentação promovem efeitos benéficos cardíacos e atenuam o dano miocárdico causado por restrição calórica em SHR, contribuindo para reduzir o risco cardiovascular e os danos morfológicos. Além disso, o ciclo de jejum/realimentação promove leve melhora do trânsito do Ca2+ e do sistema beta-adrenérgico.

Animals , Male , Papillary Muscles/metabolism , Calcium/metabolism , Fasting/physiology , Animal Nutritional Physiological Phenomena/physiology , Cardiomyopathies/prevention & control , Papillary Muscles/pathology , Rats, Inbred SHR , Time Factors , Body Weight/physiology , Calcium/analysis , Ventricular Remodeling/physiology , Caloric Restriction/adverse effects , Isoproterenol/analysis , Isoproterenol/metabolism , Myocardial Contraction , Cardiomyopathies/pathology
Acta cir. bras ; 33(6): 533-541, June 2018. graf
Article in English | LILACS | ID: biblio-949351


Abstract Purpose: To investigate the specific molecular mechanisms and effects of curcumin derivative J147 on diabetic peripheral neuropathy (DPN). Methods: We constructed streptozotocin (STZ)-induced DPN rat models to detected mechanical withdrawal threshold (MWT) in vivo using Von Frey filaments. In vitro, we measured cell viability and apoptosis, adenosine 5'-monophosphate-activated protein kinase (AMPK) and transient receptor potential A1 (TRPA1) expression using MTT, flow cytometry, qRT-PCR and western blot. Then, TRPA1 expression level and calcium reaction level were assessed in agonist AICAR treated RSC96cells. Results: The results showed that J147reduced MWT in vivo, increased the mRNA and protein level of AMPK, reduced TRPA1 expression and calcium reaction level in AITCR treated RSC96 cells, and had no obvious effect on cell viability and apoptosis. Besides, AMPK negative regulated TRPA1 expression in RSC96 cells. Conclusions: J147 could ameliorate DPN via negative regulation AMPK on TRPA1 in vivo and in vitro. A curcumin derivative J147might be a new therapeutic potential for the treatment of DPN.

Animals , Male , Curcumin/analogs & derivatives , Curcumin/pharmacology , Diabetic Neuropathies/drug therapy , AMP-Activated Protein Kinases/drug effects , TRPA1 Cation Channel/drug effects , Time Factors , Cell Survival/drug effects , Cells, Cultured , Blotting, Western , Calcium/analysis , Reproducibility of Results , Apoptosis/drug effects , Streptozocin , Diabetes Mellitus, Experimental/complications , Diabetes Mellitus, Experimental/metabolism , Diabetic Neuropathies/metabolism , AMP-Activated Protein Kinases/analysis , Real-Time Polymerase Chain Reaction , TRPA1 Cation Channel/analysis , Microscopy, Fluorescence
Arq. bras. cardiol ; 110(5): 420-427, May 2018. tab, graf
Article in English | SES-SP, LILACS, SES-SP, SESSP-IDPCPROD, SES-SP | ID: biblio-950157


Abstract Background: In view of the high mortality for cardiovascular diseases, it has become necessary to stratify the main risk factors and to choose the correct diagnostic modality. Studies have demonstrated that a zero calcium score (CS) is characteristic of a low risk for cardiovascular events. However, the prevalence of individuals with coronary atherosclerotic plaques and zero CS is conflicting in the specialized literature. Objective: To evaluate the frequency of patients with coronary atherosclerotic plaques, their degree of obstruction and associated factors in patients with zero CS and indication for coronary computed tomography angiography (CCTA). Methods: This is a cross-sectional, prospective study with 367 volunteers with zero CS at CCTA in four diagnostic imaging centers in the period from 2011 to 2016. A significance level of 5% and 95% confidence interval were adopted. Results: The frequency of atherosclerotic plaque in the coronary arteries in 367 patients with zero CS was 9.3% (34 individuals). In this subgroup, mean age was 52 ± 10 years, 18 (52.9%) were women and 16 (47%) had significant coronary obstructions (> 50%), with involvement of two or more segments in 4 (25%) patients. The frequency of non-obese individuals (90.6% vs 73.9%, p = 0.037) and alcohol drinkers (55.9% vs 34.8%, p = 0.015) was significantly higher in patients with atherosclerotic plaques, with an odds ratio of 3.4 for each of this variable. Conclusions: The frequency of atherosclerotic plaque with zero CS was relatively high, indicating that the absence of calcification does not exclude the presence of plaques, many of which obstructive, especially in non-obese subjects and alcohol drinkers.

Resumo Fundamento: Diante da alta mortalidade por doenças cardiovasculares, faz-se necessária a estratificação dos principais fatores de riscos e escolha correta da modalidade diagnóstica. Estudos demonstraram que escore de cálcio (EC) zero caracteriza baixo risco de eventos cardiovasculares. No entanto, a frequência de portadores de placa aterosclerótica coronária com EC zero é conflitante na literatura especializada. Objetivo: Avaliar a frequência de pacientes com placa aterosclerótica coronária, seu grau de obstrução e fatores associados em pacientes com EC zero e indicação para angiotomografia computadorizada de coronárias (ATCC). Métodos: Trata-se de estudo transversal, prospectivo, com 367 voluntários portadores de EC zero, mediante a ATCC, no período de 2011-16, em quatro centros de diagnóstico por imagem. Foi assumido nível de significância 5% e intervalo de confiança de 95%. Resultados: A frequência de placa aterosclerótica nas artérias coronárias dos 367 pacientes com EC zero foi de 9,3% (34 indivíduos); neste subgrupo, a média de idade foi 52 ± 10 anos, 18 (52,9%) eram mulheres e 16 (47%) exibiam obstruções coronarianas significativas (> 50%), dos quais 4 (25%) apresentaram placas em pelo menos dois segmentos. A frequência de não obesos (90,6% vs. 73,9%; p = 0,037) e de etilistas (55,9% vs. 34,8%; p = 0,015) foi significativamente maior nos portadores de placa, apresentando, cada variável, odds ratio de 3,4 para o desenvolvimento das referidas placas. Conclusões: A frequência de placa aterosclerótica com EC zero foi considerável, evidenciando, portanto, que a ausência de calcificação não exclui placa, muitas das quais obstrutivas, principalmente nos não obesos e etilistas.

Humans , Male , Female , Middle Aged , Aged , Coronary Artery Disease/diagnostic imaging , Calcium/analysis , Plaque, Atherosclerotic/diagnostic imaging , Coronary Artery Disease/complications , Coronary Artery Disease/epidemiology , Brazil/epidemiology , Biomarkers/analysis , Body Mass Index , Cross-Sectional Studies , Prospective Studies , Risk Factors , Plaque, Atherosclerotic/complications , Plaque, Atherosclerotic/epidemiology , Computed Tomography Angiography
J. bras. nefrol ; 40(1): 35-43, Jan.-Mar. 2018. tab
Article in English | LILACS | ID: biblio-893814


ABSTRACT Introduction: Obesity and Metabolic Syndrome (MS) are associated with low urinary pH and represent risk factors for nephrolithiasis, especially composed by uric acid. Acidogenic diets may also contribute to a reduction of urinary pH. Propensity for calcium oxalate precipitation has been shown to be higher with increasing features of the MS. Objective: A retrospective evaluation of anthropometric and body composition parameters, MS criteria and the dietary patterns of overweight and obese calcium stone formers and their impact upon urinary pH and other lithogenic parameters was performed. Methods: Data regarding anthropometry, body composition, serum and urinary parameters and 3-days dietary records were obtained from medical records of 102(34M/68F) calcium stone formers. Results: A negative correlation was found between urinary pH, waist circumference and serum uric acid levels (males). The endogenous production of organic acids (OA) was positively correlated with triglycerides levels and number of features of MS (males), and with glucose, uric acid and triglycerides serum levels, and number of features of MS (females). No significant correlations were detected between Net Acid Excretion (NAE) or Potential Renal Acid Load of the diet with any of the assessed parameters. A multivariate analysis showed a negative association between OA and urinary pH. Conclusion: The endogenous production of OA and not an acidogenic diet were found to be independently predictive factors for lower urinary pH levels in calcium stone formers. Hypercalciuric and/or hyperuricosuric patients presented higher OA levels and lower levels of urinary pH.

RESUMO Introdução: A obesidade e a Síndrome Metabólica (SM) se associam a pH urinário ácido e representam fatores de risco para litíase renal, especialmente a úrica. Dietas acidogênicas também podem contribuir para a redução do pH urinário. Já foi demonstrado maior risco de precipitação de oxalato de cálcio em proporção aos critérios de SM. Objetivo: Avaliar retrospectivamente o impacto de parâmetros antropométricos, composição corporal, critérios de SM e padrão alimentar sobre o pH urinário e outros parâmetros litogênicos em pacientes com sobrepeso e obesos com litíase cálcica. Métodos: Foram coletados dados de antropometria, composição corporal, exames séricos e urinários, e registros alimentares (3 dias) de 102 (34M/68F) pacientes com litíase cálcica. Resultados: O pH urinário se correlacionou negativamente com a circunferência da cintura e ácido úrico sérico (homens). A produção endógena de ácidos orgânicos (AO) se correlacionou positivamente com os triglicérides séricos e o número de critérios de SM (homens), e com glicemia, ácido úrico, triglicérides e número de critérios para SM (mulheres). Não se observaram correlações significantes entre a excreção renal líquida de ácidos (NAE) e o potencial de carga ácida renal (PRAL) da dieta com nenhum dos parâmetros avaliados. Na análise de regressão multivariada, os AO apresentaram associação negativa significante com o pH urinário. Conclusão: A produção endógena de AO, e não um padrão de dieta acidogênica, foi o fator determinante independente para menores níveis de pH urinário em pacientes com litíase cálcica. Pacientes com hipercalciúria e/ou hiperuricosúria apresentaram maiores valores de AO e menores de pH urinário.

Humans , Male , Female , Middle Aged , Uric Acid/urine , Kidney Calculi/metabolism , Calcium/metabolism , Diet , Overweight/metabolism , Kidney Calculi/complications , Kidney Calculi/urine , Kidney Calculi/chemistry , Calcium/analysis , Nutritional Status , Retrospective Studies , Overweight/complications , Overweight/urine , Obesity/complications , Obesity/metabolism , Obesity/urine
Braz. oral res. (Online) ; 32: e54, 2018. tab
Article in English | LILACS | ID: biblio-952137


Abstract Osteoporosis is an insidious and increasingly prevalent disease that can cause fractures and affect patients' quality of life. The current study comparatively evaluates patients with and without loss of bone mass in terms of salivary calcium, viscosity, and pH. A controlled cross-sectional study was conducted in two groups of 32 postmenopausal women subjected to a bone densitometry scan and later referred for dental management at the Federal University of Minas Gerais, Brazil. The patients were assigned to two groups: Group 1 - patients with low bone mineral density (BMD) and Group 2 - patients without bone mineral changes. The following salivary parameters were evaluated: calcium concentration, flow rate, viscosity, pH, and average total protein. An oral examination was performed for assessment of DMFT variables and tongue coating. Data were analyzed using descriptive and inferential statistics, adopting a p-value < 0.05. The patients' mean age was 60 years (± 7.35). Salivary flow, pH, and viscosity were similar among the groups. Average total protein was 14.8 mg/mL and 19.0 mg/mL in Groups 1 and 2, respectively. Tongue coating and salivary calcium levels were significantly higher in Group 1 (p < 0.001). Salivary calcium is an important screening tool and may eventually be used for the diagnosis of bone mineral changes.

Humans , Female , Aged , Saliva/chemistry , Osteoporosis, Postmenopausal/physiopathology , Osteoporosis, Postmenopausal/metabolism , Calcium/analysis , Oral Health , Reference Values , Tongue/physiopathology , Viscosity , Proteins/analysis , Absorptiometry, Photon , Bone Density/physiology , Case-Control Studies , DMF Index , Cross-Sectional Studies , Statistics, Nonparametric , Hydrogen-Ion Concentration , Middle Aged
J. appl. oral sci ; 26: e20170374, 2018. tab
Article in English | LILACS, BBO | ID: biblio-893735


Abstract Objectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels.

Animals , Cattle , Chelating Agents/pharmacology , Enterococcus faecalis/drug effects , Biofilms/drug effects , Dental Pulp Cavity/drug effects , Dental Pulp Cavity/microbiology , Root Canal Irrigants/pharmacology , Root Canal Irrigants/chemistry , Sodium Hypochlorite/pharmacology , Sodium Hypochlorite/chemistry , Spectrophotometry, Atomic , Materials Testing , Water/chemistry , Chelating Agents/chemistry , Calcium/analysis , Edetic Acid/pharmacology , Edetic Acid/chemistry , Enterococcus faecalis/chemistry , Dental Pulp Cavity/chemistry , Hydrogen-Ion Concentration , Ions , Iron/analysis , Manganese/analysis
J. appl. oral sci ; 25(5): 515-522, Sept.-Oct. 2017. graf
Article in English | LILACS, BBO | ID: biblio-893656


Abstract Hypersensitivity, local irritative and cytotoxic effects are known for the chemical components of Syzygium aromaticum and Cinnamomum zeylanicum contained in dental materials. However, there is no intimate data in dentistry using the whole extracts of these plants and introducing new ones. Salvia triloba is a well-known anti-inflammatory plant that correspondingly could be used in several dental traumas. Objectives: We aimed to show and compare the effect of S. aromaticum, C. zeylanicum, and S. triloba extracts on dental pulp stem cells (DPSCs) proliferation, differentiation, and immune responses. Material and Methods: Using xCELLigence, a real time monitoring system, we obtained a growth curve of DPSCs with different concentrations of the Extracts. A dose of 10 μg/mL was the most efficient concentration for vitality. Osteogenic differentiation and anti-inflammatory activities were determined by using an ELISA Kit to detect early and late markers of differentiation. Results: The level of osteonectin (ON, early osteogenic marker) decreased, which indicated that the osteogenic differentiation may be accelerated with addition of extracts. However, the level of osteocalcin (OCN, late osteogenic marker and sign of calcium granulation) differed among the extracts, in which S. aromaticum presented the highest value, followed by S. triloba and C. zeylanicum. Surprisingly, the determined calcium granules were reduced in S. aromaticum and S. triloba. In response to tumor necrosis factor alpha (TNF-α), S. triloba-treated DPSCs showed the most reduced level of IL-6 cytokine level. We suggest C. zeylanicum as a promising osteogenic inducer and S. triloba as a potent anti-inflammatory agent, which could be used safely in biocomposite or scaffold fabrications for dentistry. Conclusions: Because calcium granule formation and cell viability play a critical role in hard tissue formation, S. aromaticum in dentistry should be strictly controlled, and the mechanism leading to reduced calcium granule formation should be identified.

Humans , Adolescent , Young Adult , Drugs, Chinese Herbal/pharmacology , Plant Extracts/pharmacology , Cinnamomum zeylanicum/chemistry , Syzygium/chemistry , Dental Pulp/cytology , Mesenchymal Stem Cells/drug effects , Anti-Inflammatory Agents/pharmacology , Osteogenesis/drug effects , Time Factors , Enzyme-Linked Immunosorbent Assay , Antigens, Differentiation/analysis , Osteocalcin/analysis , Osteonectin/analysis , Cell Differentiation/drug effects , Cells, Cultured , Calcium/analysis , Reproducibility of Results , Analysis of Variance , Cytokines/analysis , Dental Pulp/drug effects , Cell Proliferation/drug effects , Flow Cytometry
Rev. Soc. Cardiol. Estado de Säo Paulo ; 27(2): 103-108, abr.-jun. 2017. ilus
Article in Portuguese | LILACS | ID: biblio-847887


A angiotomografia de coronárias emergiu recentemente como ferramenta para avaliação não invasiva da anatomia coronariana, para estratificação de risco cardiovascular e para acompanhamento dos pacientes portadores de stent e enxerto cirúrgico, fornecendo dados diagnósticos com boa acurácia. O método apresenta boa resolução temporal e espacial, o que tem contribuído para seu avanço na prática clínica. Permite visualização direta do stent, quantificação de reestenose intra-stent, além de informar sobre a patência dos enxertos cirúrgicos, entre outros propósitos. Este artigo revisa as aplicações e possíveis contribuições deste método em pacientes submetidos à revascularização miocárdica

The coronary computed tomography angiography has recently emerged as a tool for the non- -invasive evaluation of coronary anatomy, stratification of cardiovascular risk and for following up patients with coronary stent and bypass grafting, providing diagnostic data with accurate diagnostic perfomance. This method has a good temporal and spatial resolution, which has allowed advance in clinical practice. Its enables direct visualization of stent, quantification of in-stent restenosis, besides the assessment of graft patency, among others purposes. This article reviews the applications and the potencial contributions of this technique in patients submitted to myocardial revascularization

Humans , Coronary Artery Disease/mortality , Stents , Computed Tomography Angiography/methods , Myocardial Revascularization/methods , Prognosis , Prostheses and Implants , Calcium/analysis , Risk Factors , Coronary Vessels , Diagnosis , Angina Pectoris/diagnosis
Rev. Soc. Cardiol. Estado de Säo Paulo ; 27(2): 88-95, abr.-jun. 2017. ilus, graf
Article in Portuguese | LILACS | ID: biblio-847873


O escore de cálcio coronariano (CAC) é obtido por meio de tomografia de tórax sem contraste, acoplada a eletrocardiograma com baixa dose de radiação e baixo custo. Atualmente, acessível na grande maioria de tomógrafos de múltiplos detectores, o exame tem como objetivo a identificação da carga de aterosclerose associada à presença de cálcio na topografia das artérias coronárias. Seu valor diagnóstico e prognóstico para doença arterial coronária subclínica foi demonstrado em diversos estudos pregressos envolvendo dezenas de milhares de pacientes com seguimento prolongado, de até 15 anos. O escore de cálcio mostrou-se superior aos escores clínicos tradicionais na predição de eventos cardiovasculares em indivíduos assintomáticos, sobretudo naqueles com risco intermediário pré-teste, em que pode haver dúvida sobre a melhor abordagem de tratamento. O achado de um escore de cálcio zero está associado a um risco cardiovascular significativamente baixo, enquanto um escore elevado re-estratifica o indivíduo para uma classe de risco superior. Grande parte das diretrizes clínicas indica o exame exatamente neste paciente ou nos indivíduos com risco inicial mais baixo, mas com antecedente familiar fortemente positivo. Embora não haja trabalhos randomizados com desfechos clínicos primários guiados pelo CAC, ao menos três estudos prospectivos demonstraram benefícios com seu uso, além dos inúmeros trabalhos observacionais e estudos de coorte que demonstraram excelente valor prognóstico. Recentemente, recomendações de como guiar a terapia de prevenção primária de acordo com o resultado do escore de cálcio foram publicadas com base em opiniões de especialistas, e uma proposta de estudo randomizado vem sendo discutida de forma intensa. Finalmente, o escore de cálcio também passou a ser visto como possível indicação para complementar outros exames, como tomografia de tórax, avaliação pulmonar ou em associação com exames funcionais, além de aumento de adesão à terapia

The coronary artery calcium score (CAC) is obtained through a computed tomography (CT) of the chest without the need for contrast, coupled with low radiation dose electrocardiogram and low cost. The exam is currently available in most multidetector tomographs, and its objective is to identify coronary atherosclerotic burden associated with the presence of calcium in the topography of the coronary arteries. Its diagnostic and prognostic value for subclinical coronary arterial disease has been demonstrated in many studies involving tens of thousands of patients, with long follow-up times of more than 15 years. The calcium score has been shown to be superior to most traditional clinical scores for predicting cardiovascular events in asymptomatic individuals, particularly those with moderate pre-test risk, where there might be more uncertainty in selecting the best treatment approach. A finding of a calcium score of zero is associated with a very low cardiovascular risk, while a high score re-stratifies an individual to a higher risk class. Most of the clinical guidelines recommend the exam in these patients, or in individuals with an initial low risk category but a strong positive familiar history for cardiovascular disease. Although there are currently no randomized controlled trials with outcomes guided by the use of CAC, at least three prospective studies have demonstrated the benefits of its use, and numerous observational works and cohort studies have demonstrated its excellent prognostic value. Recently, recommendations on how to guide primary preventive therapy based on the results of the calcium score have been published based on specialist opinions, and a randomized controlled trial is now in advanced discussions. Finally, the calcium score is now seen as a possible indication to complement other exams, such as chest CT, pulmonary evaluation, or in association with other functional stress exams, and adhesion to therapy has increased

Humans , Tomography, X-Ray Computed/methods , Calcium/analysis , Coronary Vessels , Atherosclerosis/diagnosis , Prognosis , Radiation , Coronary Artery Disease/complications , Coronary Artery Disease/diagnosis , Calcification, Physiologic , Predictive Value of Tests , Dyslipidemias , Heart Rate
Pesqui. vet. bras ; 37(1): 17-22, jan. 2017. tab., graf.
Article in Portuguese | LILACS, VETINDEX | ID: biblio-837444


Este estudo avaliou o status do cálcio sérico em 39 vacas com deslocamento de abomaso (DA), provenientes de 30 propriedades leiteiras selecionadas, na região de Campos Gerais no Paraná. O diagnóstico do deslocamento de abomaso foi realizado por percussão auscultatória, além dos sinais clínicos e informações da anamnese. Previamente ao procedimento cirúrgico, amostras de sangue foram coletadas para a mensuração dos níveis sanguíneos de cálcio, albumina, proteína e glicose. Como grupo controle, amostras de sangue foram coletadas de vacas hígidas que se encontravam em semelhante período de lactação. Das 39 vacas com deslocamento de abomaso, 35 apresentaram hipocalcemia e no grupo controle, apenas um animal. Concentrações sanguíneas de glicose e proteína foram inferiores nos animais com DA, quando comparados com animais do grupo controle.(AU)

This study evaluated the status of serum calcium in 39 Holstein cows with displacement of the abomasum (DA), from 30 dairy farms selected in the region of Campos Gerais, Paraná state, Brazil.. The diagnosis of abomasal displacement was performed by auscultation and percussion, besides the clinical signs and history information. Before surgery, blood samples were collected to measure blood levels of calcium, albumin, protein and glucose. As a control group, blood samples were collected from healthy cows that were in similar period of lactation. Of the 39 cows with abomasal displacement, 35 had hypocalcemia and in the control group only one cow. Blood glucose and protein concentrations were lower in the animals with DA when compared with the control group.(AU)

Animals , Cattle , Abomasum/pathology , Calcium/analysis , Calcium/blood , Hypocalcemia/blood , Hypocalcemia/veterinary , Blood Glucose/analysis , Blood Proteins/analysis
Rev. Fed. Argent. Soc. Otorrinolaringol ; 24(3): 48-52, 2017. graf, tab
Article in Spanish | LILACS | ID: biblio-908156


Introducción: la causa más frecuente de vértigo en los adultos es el Vértigo Posicional Paroxístico Benigno (VPPB). La vitamina D interviene en la correcta biomineralización de la otoconia; niveles bajos de la misma y la consiguiente alteración de la homeostasis del calcio pueden alterar la formación otoconial normal, e intervenir en la aparición de dicha patología. Objetivos: Analizar la distribución de pacientes con VPPB idiopático con recurrencias según edad, sexo, valores de vitamina D total y calcemia. Determinar la frecuencia de las alteraciones de estos valores en pacientes con dicha patología. Material y método: Estudio retrospectivo, descriptivo. Resultados: 30 pacientes, con edad media de 57 años (rango: 27-87 años), de los cuales 10 (33%) fueron hombres y 20 (67%) mujeres. Del total, 29 (97%) presentaron valores normales de calcemia; sólo uno (3%) presentó hipocalcemia; 9 (30%) pacientes presentaron valores normales de vitamina D y 21 (70%), valores alterados. Del total de pacientes con hipovitaminosis D (21), 6 (29%) fueron hombres y 15 (71%) mujeres, con edad media para dicha muestra de 52 años. De la misma, el mayor número de pacientes (9 = 43%) se encontraba entre el rango de edad 47-56 años. De la población con hipovitaminosis D (21), 10 (48%) correspondieron a valores insuficientes y 11 (52%), a valores deficientes. Conclusiones: En la muestra analizada, el 70% presentó hipovitaminosis D, deficiente o insuficiente en similar proporción. Esta alteración fue más prevalente en individuos de entre 47 y 56 años, con una edad media de 52, principalmente mujeres. No se objetivaron alteraciones significativas en los valores de calcemia analizados.

Introduction: the most common cause of vertigo in adults is Benign Paroxysmal Positional Vertigo (BPPV). Vitamin D is involved in the correct biomineralization of otoconia; low levels of calcium and the consequent alteration of calcium homeostasis can alter the normal otoconial formation and to intervene on the onset of this pathology. Objectives: To analyze the distribution of patients with Idiopathic BPPV with recurrence according to age, sex, total vitamin D and calcemia. To determine frequency of the alterations of these values in patients with this pathology. Material and method: Retrospective, descriptive study. Results: 30 patients, mean age 57 years (range: 27- 87 years), 10 (33%) men and 20 (67%) women. Of the total, 29 (97%) presented normal values of calcemia, only one (3%) presented hypocalcemia. 9 (30%) patients had normal values of vitamin D and 21 (70%), altered values. Of the total number of patients with hypovitaminosis D (21), 6 (29%) were men and 15 (71%) women, mean age of 52 years. In hypovitaminosis D group (21), the age of the largest number of patients (9 = 43%) ranged from 47 to 56 years. In this group, 10 (48%) patients corresponded to insufficient vitamin D values and 11 (52%) had vitamin D deficiency. Conclusions: In our sample, 70% of patients presented insufficiency or deficiency of vitamin D values in a similar proportion. These alterations were more prevalent in individuals with age ranged from 47 to 56 years, mean age of 52, mainly women. There were no significant alterations in the analized calcemia values.

Introdução: a causa mais comum de vertigem em adultos é a Vertigem posicional paroxística benigna (VPPB). A vitamina D está envolvida na biomineralização correta da otoconia; baixos níveis de cálcio e a consequente alteração da homeostase de cálcio podem alterar a formação normal de otoconia e intervir no início desta patologia. Objetivos: Analisar a distribuição de pacientes com VPPB idiopática com recorrência de acordo com a idade, sexo, vitamina D total e calcemia. Para determinar a freqüência de alterações desses valores em pacientes com esta patologia. Material e método: Estudo descritivo, retrospectivo. Resultados: 30 pacientes, idade média de 57 anos (faixa: 27-87 anos) dos quais 10 (33%) eram homens e 20 (67%) eram mulheres. Do total, 29 (97%) apresentaram valores normais de calcemia, apenas um (3%) apresentou hipocalcemia. 9 (30%) pacientes apresentaram valores normais de vitamina D e 21 (70%), valores alterados. Do total de pacientes com hipovitaminose D (21), 6 (29%) eram homens e 15 (71%) eram mulheres, com idade média de 52 anos. Do mesmo modo, o maior número de pacientes (9 = 43%) estava entre a faixa etária de 47-56 anos. Da população com hipovitaminose D (21), 10 (48%) pacientes corresponderam a valores insuficientes de vitamina D e 11 (52%) apresentaram deficiência de vitamina D. Conclusões: Em nossa amostra, 70% dos pacientes apresentaram insuficiência ou deficiência de valores de vitamina D em proporção similar. Estas alterações foram mais prevalentes em indivíduos entre 47 e 56 anos, idade média de 52, principalmente mulheres. Não houve alterações significativas nos valores de calcemia analizados.

Male , Female , Humans , Adult , Middle Aged , Aged , Aged, 80 and over , Benign Paroxysmal Positional Vertigo/epidemiology , Benign Paroxysmal Positional Vertigo/physiopathology , Calcium Deficiency , Calcium/analysis , Hypocalcemia , Vitamin D Deficiency/epidemiology , Vitamin D/analysis
Rev. Asoc. Odontol. Argent ; 104(4): 166-174, oct.-dic. 2016. tab
Article in Spanish | LILACS | ID: biblio-869381


Objetivo: analizar bebidas y alimentos de consumo frecuente para evaluar el pH, grados Brix (ºBx), efecto buffer a pH 5,5 y 7, y concentración de fosfatos, calcio y fluoruros. Materiales y métodos: en este estudio observacional, descriptivo y transversal, se evaluaron 438 alimentos, divididos en 18 grupos. Se analizó pH, capacidad Buffer a pH 5,5 y 7, grados Brix y concentración de fosfatos, de fluoruros y de calcio. Los datos fueron procesados por SPSS Statistics 20 (IBM Corp., Estados Unidos). Resultados: las gaseosas, el jugo en polvo para diluir, las aguas saborizadas y las bebidas isotónicas presntaron menor pH. El rango de sólidos solubles, expresado en grados Brix, fue de 0,05 a 15,9. El mayor valor de efecto buffer correspondió a los jugos: exprimido, en polvo, listo para consumir y para diluir. En este último, se halló mayor cantidad de fosfato. Sólo en las aguas minerales con gas se observaron fluoruros. El alimento de soja líquido natural presentó el mayor contenido de calcio. Concusiójn: las gaseosas, los jugos, las leches fermentadas y las bebidas isotónicas, resultaron ser ácidas, con bajo efecto buffer, alto valor de grados Brix y contener pocos factores protectores.

Aims: to analyze drinks and foods commonly consumedby evaluating pH, degrees Brix, buffer effect at pH 5.5 and 7.0and phosphate, calcium and fluoride concentration.Materials and methods: In this observational, descriptiveand cross-sectional study, 48 foods were evaluated,divided into 18 groups. pH, buffer capacity at 5.5 and 7, degreesBrix and phosphate, fluoride and calcium concentrationwere analyzed. The data were processed by SPSS Statistics 20(IBM Corp., USA).Results: Soda, juice powder to dilute, flavored water andsports drinks had lower pH. The range of soluble solids (degreesBrix) was from 0.05 to 15.9. The greatest value in buffereffect was in juices: squeezed, powdered, ready to consumeand to be diluted. The latter had a higher amount of phosphate.Only sparkling mineral waters showed detectable valuesfor fluorides. The liquid natural soy food had the highestcalcium content.Conclusion: Soft drinks, juices, fermentable milk, isotonicdrinks were found to be acidic, with lower buffer value,high Brix degrees and low in protective factors.

Humans , Food and Beverages/analysis , Dental Caries/etiology , Risk Factors , Tooth Erosion , Breast-Milk Substitutes , Carbonated Beverages , Cross-Sectional Studies , Calcium/analysis , Fluorides/analysis , Phosphates/analysis , Juices , Mineral Waters , Soy Milk , Statistical Analysis