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1.
Autops. Case Rep ; 11: e2021276, 2021. tab, graf
Article in English | LILACS | ID: biblio-1249019

ABSTRACT

Adamantinoma of the long bones is an exceedingly rare and slow-growing tumor that affects the diaphysis of long bones, particularly the tibia. Based on the pattern of the epithelial cell component and the presence or absence of the osteofibrous dysplasia-like element, several histological variants have been described, such as (i) tubular (the most frequent), (ii) basaloid, (iii) squamous, (iv) spindle variant, (v) osteofibrous dysplasia -like variant, and (vi) Ewing's sarcoma - like adamantinoma (the least frequent). The diagnosis may be challenging since this tumor may be mistakenly interpreted as carcinoma, myoepithelial tumor, osteofibrous dysplasia, and vascular tumor. We report the case of a 41-year-old male who presented with swelling over the right leg associated with pain. The X-ray showed a lytic lesion of the right-sided tibia. The diagnosis of adamantinoma was made based on the clinico-radiological, histomorphology, and immunohistochemical findings. Histologically, classic adamantinoma is a biphasic tumor characterized by epithelial and osteofibrous components in varying proportions and differentiating patterns. The diagnosis can be confirmed by immunohistochemistry for demonstrating sparse epithelial cell nests when the radiological features are strongly consistent with adamantinoma. This case is highlighted because the epithelial component can lead to a misdiagnosis, particularly when the clinico-radiological features are overlooked. Adamantinoma of long bones has the potential for local recurrence and may metastasize to the lungs, lymph nodes, or other bones. The prognosis is good if early intervention is taken.


Subject(s)
Humans , Male , Adult , Tibia/pathology , Adamantinoma/pathology , Diaphyses
2.
Arq. bras. med. vet. zootec. (Online) ; 72(6): 2259-2265, Nov.-Dec. 2020. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1142302

ABSTRACT

A osteomielite é um desafio terapêutico em ortopedia, capaz de retardar ou mesmo impedir a consolidação óssea. O omento, há anos, tem sido empregado como alternativa em diferentes procedimentos cirúrgicos, por sua capacidade, entre outras, de angiogênese, sendo aplicado na ortopedia veterinária quando há o risco de não união óssea. Neste caso, um cão Fila Brasileiro foi submetido à realização de enxerto com retalho pediculado de omento maior, após osteomielite resistente presente em osteossíntese de fratura múltipla de tíbia aberta grau II. Durante 16 dias, manteve-se a comunicação do retalho, mas, diante do risco de peritonite, o pedículo foi seccionado. Numa sequência de intervenções cirúrgicas, após 89 dias, houve cicatrização óssea e remissão da osteomielite, mesmo na presença de bactérias multirresistentes. Neste relato, o omento foi efetivo como terapia adjuvante no tratamento da osteomielite e garantiu o retorno da função do membro.(AU)


Osteomyelitis is a therapeutic challenge in orthopedics, capable of delaying or even preventing bone healing. The omentum has been used in different surgical procedures as an alternative for its capacity, among others, of angiogenesis, being applied in veterinary orthopedics, when there is a risk of non-union of bone. In this case, a Brazilian row dog was submitted to grafting with pedicle flap of greater omentum, after resistant osteomyelitis present in open fracture osteosynthesis of open tibia grade II. For 16 days the communication of the flap was maintained, but at the risk of peritonitis, the pedicle was sectioned. In a sequence of surgical interventions, after 89 days, there was bone healing and remission of osteomyelitis, even in the presence of multi-resistant bacteria. In this report, the omentum was effective as adjuvant therapy in the treatment of osteomyelitis and guaranteed the return of limb function.(AU)


Subject(s)
Animals , Dogs , Omentum/transplantation , Osteomyelitis/therapy , Osteomyelitis/veterinary , Tibia/pathology , Pedicle Screws/veterinary
3.
Int. j. morphol ; 38(5): 1398-1404, oct. 2020. tab, graf
Article in English | LILACS | ID: biblio-1134455

ABSTRACT

SUMMARY: The objective of this study were bone defect complications that occur due to traumas or infections. Bone grafts are required to provide support, fill gaps and improve biological repair in skeletal damage. Dexamethasone plays role in calcium signaling modulation and used in diseases. Aim of this study was to evaluate osteonectin and osteopontin expressions in new bone development after dexamethasone application on tibial bone defects. Rats were divided into defect, defect+graft and defect+graft+dexamethasone treated groups. Tibial bone defect created, and rats were kept immobile for 28 days. Alloplastic material was placed in defect area in second and group third groups. 2.5 mg/kg Dex and normal saline were injected to dexamethasone and defect groups twice a week for 56 days. Inflammation and congestion were increased in defect and defect+graft groups. Defect+graft+dexamethasone group; increased number of osteoblast and osteocyte cells, dense bone matrix, formation of new bone trabeculae was observed. Defect+graft group; osteonectin expression in graft regions, osteoblast cells, some connective tissue cells and fibers were seen whereas in defect+graft+dexamethasone group; osteopontin expression in osteoblast and osteocyte cells of new bone trabeculae were observed. Dexamethasone may lead to formation of new bone trabeculae into the graft material resulting in increased osteoconduction and osteoinductive effect for differentiation of osteon.


RESUMEN: Los defectos óseos son complicaciones que ocurren debido a traumas o infecciones. Se requieren injertos óseos para proporcionar apoyo, llenar los espacios y mejorar la reparación biológica en el hueso dañado. La dexametasona desempeña un papel importante en la modulación de la señalización del calcio y se usa en enfermedades. El objetivo de este estudio fue evaluar las expresiones de osteonectina y osteopontina en el desarrollo óseo después de la aplicación de dexametasona en defectos óseos tibiales. Las ratas se dividieron en grupos: defecto, defecto + injerto y defecto + injerto + grupos tratados con dexametasona. Se creó un defecto óseo tibial, y las ratas se mantuvieron inmóviles durante 28 días. El material aloplástico se colocó en el área del defecto en el segundo y tercer grupo. Se inyectaron 2,5 mg / kg de dexametasona y solución salina normal a grupos de defectos dos veces por semana durante 56 días. La inflamación y la congestión aumentaron en los grupos de defectos y defectos + injerto; En el grupo defecto + injerto + grupo tratado con dexametasona se observó un aumento en el número de osteoblastos y osteocitos, de matriz ósea densa y en la formación de nuevas trabéculas óseas. En el grupo defecto + grupo de injerto se observó la expresión de osteonectina en las áreas de injerto, osteoblastos, algunas células y fibras de tejido conectivo, mientras que en el grupo defecto + injerto + dexametasona se observó la expresión de osteopontina en osteoblastos y osteocitos y formación de nuevas trabéculas óseas . En conclusión la dexametasona puede conducir a la formación de nuevas trabéculas óseas en el material de injerto, lo que resulta en un aumento de la osteoconducción y un efecto osteoinductivo para la diferenciación del osteón.


Subject(s)
Animals , Male , Rats , Tibia/surgery , Tibia/drug effects , Dexamethasone/administration & dosage , Bone Transplantation , Tibia/pathology , Bone Regeneration , Immunohistochemistry , Osteonectin/physiology , Bone Remodeling , Rats, Wistar , Disease Models, Animal , Osteopontin/physiology
4.
Int. j. morphol ; 38(1): 43-47, Feb. 2020. tab, graf
Article in English | LILACS | ID: biblio-1056395

ABSTRACT

Rheumatoid arthritis (RA) is a disease triggered by environmental and genetic factors. Research suggests that physical exercise has benefits such as delaying functional disability. In vivo studies using experimental models of arthritis can provide useful information about these benefits. to analyze the effects that different intensities of aquatic physical exercise have on the proprieties of the bones in induced arthritis in knees of Wistar rats. Male Wistar adults rats (n=20) were divided into 5 groups: Group Control Arthritis (GCA) n=4, Group control Placebo (GCP) n=4, Group Low Physical Activity (GB) n=4, Group Moderate Physical Activity (GM) n=4 and Group Intense Physical Activity (GI) n=4. The physical activity groups got an intra-articular injection of Zymosam on the right knee; the GCA received saline solution in the right knee; the GCP was submitted to the stress of the needle. The animals were submitted to aquatic activity for 30 minutes, 4 times a week for 5 weeks, and the intensity of the exercise was determined by a weight placed on their back: GB=1 %, GM=5 %, GI=15 % of their body weight. It was observed that the group GB, and the groups that did not exercise GCA and GCP, gained more weight compared to the group GM. In relation to the bone mineral content of the tibia, there was a decrease in the GM group when compared to the GCP group, whereas in the tibial bone mineral density there was a decrease in the GM group compared to the GCP, GCA, GB. As for the area of the femur, the GI group presented an increase of it compared to the GB and GM groups. It is concluded that the high intensity exercises promote better results in bone properties.


La investigación sugiere que el ejercicio físico tiene beneficios como retrasar la discapacidad funcional de la artritis reumatoide. Los estudios in vivo que utilizan modelos experimentales de artritis pueden proporcionar información útil sobre estos beneficios. Se analizaron los efectos de las intensidades del ejercicio físico acuático sobre las propiedades de los huesos, en la artritis inducida en las rodillas de ratas Wistar. Las ratas Wistar macho adultas (n = 20) se dividieron en 5 grupos: grupo de control artritis (ACG) n = 4, grupo control placebo (CGP) n = 4, grupo de actividad física baja (GB) n = 4, grupo de actividad física moderada (GM) n = 4 y grupo de actividad física intensa (GI) n = 4. Los grupos de actividad física recibieron una inyección intraarticular de Zymosam en la rodilla derecha; el GCA recibió solución salina en la rodilla derecha; el CGP fue sometido a la tensión de una aguja. Los animales fueron sometidos a actividad acuática durante 30 minutos, 4 veces a la semana durante 5 semanas, y la intensidad del ejercicio se determinó mediante un peso colocado sobre su espalda: GB = 1 %, GM = 5 %, GI = 15 % de su peso corporal. Se observó que el grupo GB, y los grupos que no ejercitaron GCA y CGP, ganaron más peso en comparación con el grupo GM. En relación con el contenido mineral óseo de la tibia, hubo una disminución en el grupo GM en comparación con el grupo GCP, mientras que en la densidad mineral del hueso tibial hubo una disminución en el grupo GM en comparación con el GCP, GCA, GB. En cuanto al área del fémur, el grupo GI presentó un aumento en comparación con los grupos GB y GM. En conclusión el ejercicio de alta intensidad promueve mejores resultados en las propiedades óseas.


Subject(s)
Animals , Male , Rats , Arthritis, Rheumatoid/pathology , Swimming/physiology , Tibia/pathology , Femur/pathology , Arthritis, Rheumatoid/physiopathology , Tibia/physiopathology , Body Weight , Exercise/physiology , Rats, Wistar , Disease Models, Animal , Femur/physiopathology
5.
Braz. oral res. (Online) ; 34: e008, 2020. tab, graf
Article in English | LILACS | ID: biblio-1089382

ABSTRACT

Abstract This study aimed to investigate the effects of chronic restraint stress (RS) and a high-fat diet (HFD) on the osseointegration of titanium implants in a rat model. After the surgical insertion of titanium implants into the metaphysis of the tibial bone, the rats were randomly divided into four equal groups (n = 8 each): control (CNT), restraint stress (RS), high-fat diet (HFD), and restraint stress plus high fat diet (RS-HFD). CNT: Rats received no further treatment during the 92-day experimental period. RS: Stress was applied to the rats beginning from two days after the implant surgery for one hour per day for the first 30 days, two hours per day for the next 30 days, and three hours per day for the last 30 days. HFD: Rats were fed a HFD for the following 90 days starting two days after surgery. RS-HFD: Rats were fed a HFD and RS was applied to rats for the following 90 days, starting two days after surgery. At the end of the experimental period, the rats were euthanized, and the implants and surrounding bone tissues were removed for histological analysis. Statistical analysis was performed by one way ANOVA and Bonferrroni tests. There were no significant differences in the bone-implant connection levels between the groups (p > 0.05), but in the HFD and RS-HFD groups, the bone filling ratios were found to be lower compared with the controls (p < 0.05) The data analyzed in this study suggest that an HFD with or without chronic RS adversely affected bone tissue in the rats during the 90-day osseointegration period.


Subject(s)
Animals , Female , Stress, Psychological/physiopathology , Tibia/physiopathology , Titanium , Osseointegration/physiology , Diet, High-Fat/psychology , Bone-Anchored Prosthesis , Aspartate Aminotransferases/blood , Reference Values , Tibia/surgery , Tibia/pathology , Time Factors , Triglycerides/blood , Blood Glucose/analysis , Random Allocation , Cholesterol/blood , Reproducibility of Results , Rats, Sprague-Dawley , Dental Implantation, Endosseous/methods , Alanine Transaminase/blood
6.
Braz. oral res. (Online) ; 34: e008, 2020. tab, graf
Article in English | LILACS | ID: biblio-1055524

ABSTRACT

Abstract This study aimed to investigate the effects of chronic restraint stress (RS) and a high-fat diet (HFD) on the osseointegration of titanium implants in a rat model. After the surgical insertion of titanium implants into the metaphysis of the tibial bone, the rats were randomly divided into four equal groups (n = 8 each): control (CNT), restraint stress (RS), high-fat diet (HFD), and restraint stress plus high fat diet (RS-HFD). CNT: Rats received no further treatment during the 92-day experimental period. RS: Stress was applied to the rats beginning from two days after the implant surgery for one hour per day for the first 30 days, two hours per day for the next 30 days, and three hours per day for the last 30 days. HFD: Rats were fed a HFD for the following 90 days starting two days after surgery. RS-HFD: Rats were fed a HFD and RS was applied to rats for the following 90 days, starting two days after surgery. At the end of the experimental period, the rats were euthanized, and the implants and surrounding bone tissues were removed for histological analysis. Statistical analysis was performed by one way ANOVA and Bonferrroni tests. There were no significant differences in the bone-implant connection levels between the groups (p > 0.05), but in the HFD and RS-HFD groups, the bone filling ratios were found to be lower compared with the controls (p < 0.05) The data analyzed in this study suggest that an HFD with or without chronic RS adversely affected bone tissue in the rats during the 90-day osseointegration period.


Subject(s)
Humans , Animals , Stress, Psychological/physiopathology , Tibia/physiopathology , Titanium , Osseointegration/physiology , Diet, High-Fat/psychology , Bone-Anchored Prosthesis , Aspartate Aminotransferases/blood , Reference Values , Tibia/surgery , Tibia/pathology , Time Factors , Triglycerides/blood , Blood Glucose/analysis , Random Allocation , Cholesterol/blood , Reproducibility of Results , Rats, Sprague-Dawley , Dental Implantation, Endosseous/methods , Alanine Transaminase/blood
7.
J. appl. oral sci ; 27: e20180103, 2019. graf
Article in English | LILACS, BBO | ID: biblio-1002400

ABSTRACT

Abstract Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera. Material and Methods Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon®; G3) Hemospon® in a culture medium enriched with 8% Aloe vera; G4) Hemospon® in a culture medium containing hDPSCs and G5) Hemospon® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn's post-test. Results On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon® (p<0.05). No statistically significant difference was found between the groups regarding bone formation at the three experimental points in time. Osteopontin expression corroborated the intensity of bone formation. Fluorescence microscopy revealed positive labeling with Q-Tracker® in hDPSCs before transplantation and tissue repair. Conclusion The results suggest that the combination of Hemospon®, Aloe vera and hDPSCs is a form of clinical treatment for the repair of non-critical bone defects that reduces the inflammatory cascade's effects.


Subject(s)
Humans , Animals , Male , Rats , Bone Regeneration/drug effects , Bone Regeneration/physiology , Plant Extracts/pharmacology , Dental Pulp/cytology , Mesenchymal Stem Cell Transplantation/methods , Aloe/chemistry , Osteogenesis/drug effects , Osteogenesis/physiology , Tibia/drug effects , Tibia/physiology , Tibia/pathology , Time Factors , Immunohistochemistry , Hemostatics/pharmacology , Cell Survival/drug effects , Cell Survival/physiology , Cells, Cultured , Reproducibility of Results , Collagen/pharmacology , Treatment Outcome , Osteopontin/analysis , Flow Cytometry , Microscopy, Fluorescence
8.
Acta cir. bras ; 34(4): e201900408, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001086

ABSTRACT

Abstract Purpose: To evaluate histologically and immunohistochemically the bone regeneration after application of simvastatin on tibial bone defects in rats. Methods: Sixty Wistar albino rats were divided into 3 groups as control (6 mm tibial bone defect), defect + graft (allograft treatment), and defect + graft + simvastatin (10 mg/kg/day) for 28 days. Results: Histopathological examination revealed inflammation in control group (defect group), congestion in blood vessels, and an increase in osteoclast cells. In defect + graft group, osteoclastic activity was observed and osteocyte cells were continued to develop. In defect + graft + simvastatin group, osteocytes and matrix formation were increased in the new bone trabeculae. Osteopontin and osteonectin expression were positive in the osteclast cells in the control group. Osteoblasts and some osteocytes showed a positive reaction of osteopontin and osteopontin. In defect + graft + simvastatin group, osteonectin and osteopontin expression were positive in osteoblast and osteocyte cells, and a positive expression in osteon formation was also seen in new bone trabeculae. Conclusion: The simvastatin application was thought to increase bone turnover by increasing the osteoinductive effect with graft and significantly affect the formation of new bone.


Subject(s)
Animals , Male , Rats , Tibia/drug effects , Bone Regeneration/drug effects , Simvastatin/pharmacology , Osteoblasts , Osteoclasts , Tibia/surgery , Tibia/pathology , Bone Remodeling/drug effects , Rats, Wistar , Disease Models, Animal , Autografts
9.
Rev. Asoc. Argent. Ortop. Traumatol ; 83(4): 268-273, dic. 2018. []
Article in Spanish | LILACS, BINACIS | ID: biblio-984993

ABSTRACT

Introducción: Las fracturas de ambos platillos tibiales ocurren por traumatismos de alta energía. A veces, pueden comprometer el tubérculo anterior de la tibia. Ni la clasificación de Schatzker ni la de la AO/OTA consideran la presencia de este fragmento. El objetivo de este estudio fue describir la incidencia y el manejo quirúrgico de este tipo de fracturas. Materiales y Métodos: Se realizó un estudio retrospectivo, entre 2009 y 2017, que incluyó 48 fracturas de ambos platillos tratadas con reducción y osteosíntesis, 10 presentaban un fragmento de la tuberosidad anterior asociado. Siete pacientes eran hombres y 3, mujeres. La edad promedio era de 33.5 años. Nueve fueron estabilizadas inicialmente con tutor externo. El fragmento de la tuberosidad se fijó con 2 tornillos de 3,5 mm (9 casos) y con una placa 1/3 de tubo (un caso). Un paciente tenía una fractura expuesta. El seguimiento mínimo fue de 12 meses. Resultados: En todos, se logró la consolidación ósea. No hubo infecciones. El rango de movilidad de la rodilla logrado fue de 10º de extensión (rango 0-20º) a 120º de flexión (rango 90-140º). En un caso fue necesario retirar el material de osteosíntesis. Un paciente requirió artroscopia para tratar una lesión meniscal. Conclusiones: El 20,8% de las fracturas de ambos platillos tibiales presenta un fragmento de la tuberosidad anterior de la tibia, según nuestra serie. La fijación de este fragmento con tornillos de 3,5 mm o una placa 1/3 de tubo bloqueada es una técnica eficaz para lograr una estabilidad adecuada del fragmento. Nivel de Evidencia: IV


Introduction: Tibial plateau fractures are secondary to high-energy traumas. Sometimes, tibial tubercle is involved. Neither Schatzker nor AO/OTA classifications consider tibial tubercle involvement. The aim of this study was to describe the incidence and management of these fractures. Methods: A retrospective study was performed between 2009 and 2017. Ten out of 48 tibial plateau fractures treated with internal fixation during this period had tibial tubercle involvement. There were 7 men and 3 women, with a mean age of 33.5 years. Nine were initially treated with an external fixation. Tibial tubercle was fixed with two 3.5 mm screws in 9 patients and with a 1/3 tubular plate in only one. There was one open fracture. Minimum follow-up lasted 12 months. Results: All patients achieved bone consolidation. There were no infections. Achieved range of motion was 10º of extension (range 0-20º) and 120º of flexion (range 90-140º). In one patient it was necessary to withdraw the implant. One patient underwent knee athroscopy because of a meniscal injury. Conclusions: According to our series, tibial plateau fractures associate with tibial tubercle involvement in 20.8% of cases. Fixation with 3.5 mm screws or 1/3 tubular plate is a reliable option to treat tibial tubercle involvement. Level of Evidence: IV


Subject(s)
Adult , Tibia/pathology , Tibial Fractures/surgery , Fracture Fixation, Internal/methods , Knee Injuries , Follow-Up Studies , Treatment Outcome
10.
J. appl. oral sci ; 26: e20170470, 2018. graf
Article in English | LILACS, BBO | ID: biblio-954503

ABSTRACT

Abstract The hypothesis of this study was that the peri-implant bone healing of the group of pinealectomized rats would differ from the control group. The samples were subjected to immunohistochemical, microtomographic (total porosity and connectivity density), and fluorochrome (mineralized surface) analyses. Objectives The goal of this study was to investigate the cellular changes and bone remodeling dynamics along the bone/implant interface in pinealectomized rats. Material and Methods The total of 18 adult male rats (Rattus norvegicus albinus, Wistar) was divided into three groups (n=6): control (CO), pinealectomized without melatonin (PNX) and pinealectomized with melatonin (PNXm). All animals were submitted to the first surgery (pinealectomy), except the CO group. Thirty days after the pinealectomy without melatonin, the second surgery was conducted, in which all animals received an implant in each tibia (36 titanium implants with surface treatment were installed - Implalife® São Paulo, SP, Brazil). By gavage, the rats of the PNX group received the vehicle solution, and the procedure. Results Immunohistochemical analysis for runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteopontin (OP) and osteocalcin (OC) showed that the bone repair process in the PNXm group was similar to that of the CO group, whereas the PNX group showed a delay. The microtomographic parameters of total porosity [Po(tot)] and bone surface (BS) showed no statistically significant differences, whereas for the connective density (Conn.Dn) a statistical difference was found between the CO and PNXm groups. Fluorochrome analysis of the active mineralized surface showed statistically significant difference between the CO and PNX and between the CO and PNXm groups. Conclusion The absence of the pineal gland impaired the bone repair process during osseointegration, however the daily melatonin replacement was able to restore this response.


Subject(s)
Animals , Male , Pineal Gland/surgery , Osseointegration/drug effects , Bone Density Conservation Agents/pharmacology , Bone-Implant Interface , Melatonin/pharmacology , Tibia/drug effects , Tibia/injuries , Tibia/pathology , Titanium , Immunohistochemistry , Osteocalcin/analysis , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Implants, Experimental , Dental Implantation, Endosseous , Alkaline Phosphatase/analysis , Core Binding Factor Alpha 1 Subunit/analysis , Osteopontin/analysis , X-Ray Microtomography , Fluorescent Dyes
11.
Acta cir. bras ; 32(9): 767-780, Sept. 2017. tab, graf
Article in English | LILACS | ID: biblio-886243

ABSTRACT

Abstract Purpose: To assess the efficacy of allogeneic mesenchymal stem cells and xenogenic platelet rich plasma in the treatment of bone failure of osteoporotic rabbits secondary to estrogenic deprivation and iatrogenic hypercortisolism. Methods: Eight female rabbits underwent ovarian resection and corticoid therapy to induce clinical status of osteoporosis. Four failures were produced in the tibiae, with each failure being treated with hemostatic sponge, allogenic mesenchymal stem cells, xenogenic platelet-rich plasma and the association between both. The animals were divided into two groups, evaluated radiographically and histopathologically at 30 and 60 days post treatment. Results: A radiographically confirmed consolidation of bone failures treated with allogeneic mesenchymal stem cells, associated with the histopathological image of mature and immature bone tissue, without evidence of osteopenia, was compared with the other groups, in which radiolucent failures with osteopenia and fibrosis were still present, denoting the satisfactory effect of the first treatment in detriment to the others. Conclusion: The treatment of bone failures of rabbits with secondary osteoporosis with allogeneic mesenchymal stem cells induced greater bone consolidation with mature and immature bone tissue production (p<0.01), when compared to the other treatments.


Subject(s)
Animals , Female , Rats , Osteoporosis/complications , Tibia/pathology , Bone Regeneration/physiology , Mesenchymal Stem Cell Transplantation , Platelet-Rich Plasma , Tibia/injuries , Time Factors , Transplantation, Heterologous
12.
Rev. Assoc. Med. Bras. (1992) ; 63(4): 303-306, Apr. 2017. graf
Article in English | LILACS | ID: biblio-842543

ABSTRACT

Summary Chronic recurrent multifocal osteomyelitis is an idiopathic nonpyogenic autoinflammatory bone disorder involving multiple sites, with clinical progression persisting for more than 6 months and which may have episodes of remission and exacerbation in the long term. It represents up to 2-5% of the cases of osteomyelitis, with an approximate incidence of up to 4/1,000,000 individuals, and average age of disease onset estimated between 8-11 years, predominantly in females. The legs are the most affected, with a predilection for metaphyseal regions along the growth plate. We describe the case of a female patient, aged 2 years and 5 months, with involvement of the left ulna, right jaw and left tibia, showing a predominance of periosteal reaction as main finding.


Resumo Osteomielite crônica multifocal recorrente é uma desordem autoinflamatória óssea idiopática não piogênica, envolvendo vários sítios e com evolução clínica persistindo por mais de 6 meses, podendo apresentar episódios de remissão e exacerbação em longo prazo. Representa de 2 a 5% das osteomielites, com incidência aproximada de até 4/1.000.000, com idade média de apresentação estimada entre 8 e 11 anos, predominando no gênero feminino. Os membros inferiores são os mais afetados, com predileção para regiões metafisárias junto à fise. Descrevemos um caso da doença em uma menina de 2 anos e 5 meses de idade, com acometimento de ulna esquerda, mandíbula à direita e tíbia esquerda, exibindo predomínio de reação periosteal como achado principal.


Subject(s)
Humans , Female , Child, Preschool , Osteomyelitis/pathology , Osteomyelitis/diagnostic imaging , Periostitis/pathology , Periostitis/diagnostic imaging , Tibia/pathology , Tibia/diagnostic imaging , Ulna/pathology , Ulna/diagnostic imaging , Biopsy , Tomography, X-Ray Computed , Mandible/diagnostic imaging
13.
Int. j. morphol ; 35(1): 178-183, Mar. 2017. ilus
Article in English | LILACS | ID: biblio-840951

ABSTRACT

The aim of this study is evaluate the efficacy of 904 nm laser diode in bone regeneration in the bone defect in diabetic rats. Six groups of 10 male Wistar rats and 2 mm bone defects drilled on the left and right tibia were used. The diabetic animals were treated with streptozotocin (40 mg/kg, i.v.). We compared the diode laser doses of treatment of bone defects 50 w ­ 4 J/cm and 100 w ­ 4J/. The right tibia was used for immunohistochemical analysis with the apoptosis markers XIAP and Caspase-3 and the left tibia was submitted to computer tomography (CT). Caspase-3 marker showed greater amount of apoptosis in all the untreated groups compared to both laser treatments. There was no statistical significance for XIAP marker. CT scan showed improvement of bone defect area and volume in both laser treated groups, control and diabetic. Therefore the low intensity laser therapy was effective in accelerating bone repair in both, control and diabetic groups. It was evidenced in our study that diabetes influences bone repair negatively.


Los objetivos de este estudio fueron evaluar la eficacia del láser diodo de 904 nm en la regeneración ósea del defecto óseo en ratas diabéticas. Se utilizaron seis grupos de 10 ratas Wistar macho y se generó un defecto óseo de 2 mm en las tibias izquierda y derecha de los animales. El animal diabético fue generado con estreptozotocina (40 mg / kg, i.v.). Se compararon las dosis de tratamiento de los defectos óseos con láser de diodo de 50 w - 4 J / cm y 100w - 4 J /. La tibia derecha fue utilizada para el análisis inmunohistoquímico con los marcadores de apoptosis XIAP y Caspasa-3 y la tibia izquierda fue sometida a tomografía computarizada. El marcador caspasa-3 mostró mayor cantidad de apoptosis en todos los grupos no tratados en comparación con ambos tratamientos con láser. No hubo significación estadística para el marcador XIAP. La tomografía computarizada mostró una mejoría del área y el volumen de los defectos óseos en ambos grupos tratados con láser, control y diabéticos. Por lo tanto, la terapia con láser de baja intensidad fue eficaz en la aceleración de la reparación ósea tanto en los grupos control como en los diabéticos. Se evidenció en nuestro estudio que la diabetes afecta negativamente la reparación ósea.


Subject(s)
Animals , Male , Rats , Bone and Bones/pathology , Bone and Bones/radiation effects , Diabetes Mellitus , Low-Level Light Therapy , Apoptosis , Bone Regeneration/radiation effects , Immunohistochemistry , Rats, Wistar , Tibia/pathology , Tibia/radiation effects
14.
Braz. oral res. (Online) ; 31: e7, 2017. tab, graf
Article in English | LILACS | ID: biblio-839537

ABSTRACT

Abstract The aim of this study was to evaluate the efficacy of low-level 940 nm laser therapy with energy intensities of 5, 10 and 20 J/cm2 on bone healing in an animal model. A total of 48 female adult Wistar rats underwent surgery to create bone defects in the right tibias. Low-level laser therapy (LLLT) was applied immediately after surgery and on post-operative days 2, 4, 6, 8, 10 and 12 in three study groups with energy intensities of 5 J/cm2, 10 J/cm2 and 20 J/cm2 using a 940 nm Gallium-Aluminium-Arsenide (Ga-Al-As) laser, while one control group underwent only the tibia defect surgery. All animals were sacrificed 4 or 8 weeks post-surgery. Fibroblasts, osteoblasts, osteocytes, osteoclasts and newly formed vessels were evaluated by a histological examination. No significant change was observed in the number of osteocytes, osteoblasts, osteoclasts and newly formed vessels at either time period across all laser groups. Although LLLT with the 10 J/cm2 energy density increased fibroblast activity at the 4th week in comparison with the 5 and 20 J/cm2 groups, no significant change was observed between the laser groups and the control group. These results indicate that low-level 940 nm laser with different energy intensities may not have marked effects on the bone healing process in both phases of bone formation.


Subject(s)
Animals , Female , Wound Healing/radiation effects , Bone Regeneration/radiation effects , Low-Level Light Therapy/methods , Lasers, Semiconductor/therapeutic use , Osteoblasts/radiation effects , Osteoclasts/radiation effects , Osteogenesis/radiation effects , Reference Values , Tibia/radiation effects , Tibia/pathology , Time Factors , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Dose-Response Relationship, Radiation , Fibroblasts/radiation effects
16.
J. appl. oral sci ; 24(3): 239-249, tab, graf
Article in English | LILACS, BBO | ID: lil-787550

ABSTRACT

ABSTRACT Diabetes mellitus (DM) causes an increased production of free radicals that can impair bone healing. Melatonin is a hormone secreted mainly by the pineal gland, which participates in the neutralization process of free radicals. Objective The aim of this study was to investigate histologic and biochemical effects of supplemental melatonin administration on bone healing and antioxidant defense mechanism in diabetic rats. Material and Methods Eighty-six Sprague-Dawley male rats were used in this study. Diabetes mellitus was induced by intraperitoneal (i.p.) administration of 65 mg/kg streptozotocin (STZ). Surgical bone defects were prepared in the tibia of each animal. Diabetic animals and those in control groups were treated either with daily melatonin (250 μg/animal/day/i.p.) diluted in ethanol, only ethanol, or sterile saline solution. Rats were humanely killed at the 10th and 30th postoperative days. Plasma levels of Advanced Oxidation Protein Products (AOPP), Malondialdehyde (MDA), and Superoxide Dismutase (SOD) were measured. The number of osteoblasts, blood vessels and the area of new mineralized tissue formation were calculated in histologic sections. Results At the 10th day, DM+MEL (rats receiving both STZ and melatonin) group had significantly higher number of osteoblasts and blood vessels as well as larger new mineralized tissue surfaces (p<0.05 for each) when compared with DM group. At the 30th day, DM group treated with melatonin had significantly lower levels of AOPP and MDA than those of DM group (p<0.05). Conclusion Melatonin administration in STZ induced diabetic rats reduced oxidative stress related biomarkers and showed beneficial effects on bone healing at short term.


Subject(s)
Animals , Male , Free Radical Scavengers/administration & dosage , Fracture Healing/drug effects , Diabetes Mellitus, Experimental/metabolism , Melatonin/administration & dosage , Osteoblasts/drug effects , Reference Values , Superoxide Dismutase/blood , Tibia/drug effects , Tibia/pathology , Time Factors , Fibrosis , Calcification, Physiologic/drug effects , Biomarkers , Cell Count , Reproducibility of Results , Rats, Sprague-Dawley , Streptozocin , Oxidative Stress/drug effects , Diabetes Mellitus, Experimental/chemically induced , Advanced Oxidation Protein Products/blood , Malondialdehyde/blood
17.
Int. j. morphol ; 34(2): 763-769, June 2016. ilus
Article in English | LILACS | ID: lil-787066

ABSTRACT

The aim of this study was to evaluate the effects of melatonin healing in a tibial bone defect model in rats by means of histopathological and immunohistochemistry analysis. Twenty one male Wistar albino rats were used in this study. In each animal, bone defects (6 mm length ) were created in the tibias. The animals were divided into three groups. In group 1 control group (rats which tibial defects). Group 2 melatonin (10 mg/kg) + 14 days in the tibial defect group) was administered intraperitoneally to rats. Group 3 melatonin (10 mg/kg) + 28 days in the tibial defect group) was administered intraperitoneally to rats. Histopathological analysis of samples was performed to evaluate the process of osteoblastic activity, matrix formation, trabecular bone formation and myeloid tissue in bone defects. Immunohistochemical and immunoblot analysis demonstrated non-collagenous proteins (osteopontin and osteonectin) differences in tibial bone defects. The expression of osteopontin on tibia was increased by 14 days melatonin treatment. The expression of osteonectin on tibia was dramatically increased by 14 days melatonin treatment.


El objetivo fue evaluar por medio de análisis histopatológico e inmunohistoquímico los efectos cicatrizantes de la melatonina en un modelo de defecto óseo tibial en ratas. Se utilizaron 21 ratas albinas Wistar macho. En cada animal, se crearon defectos óseos en las tibias de 6 mm de longitud. Los animales se dividieron en tres grupos. El Grupo 1 correspondió al grupo control (defectos tibiales sin tratamiento). Al Grupo 2 se administró melatonina por vía intraperitoneal (10 mg/kg) 14 días posteriores al defecto tibial. Al Grupo 3 se administró melatonina por vía intraperitoneal (10 mg/kg) 28 días posteriores al defecto tibial. Se realizó un análisis histopatológico para evaluar los procesos de actividad osteoblástica, formación de matriz, formación de hueso trabecular y tejido mieloide en los defectos óseos. Los análisis inmunohistoquímicos y de inmunotransferencia mostraron diferencias de proteínas no colágenas (osteopontina y osteonectina). La expresión de osteopontina en defectos óseos tibiales se incrementó en el Grupo 2. La expresión de osteonectina en la tibia se incrementó fuertemente bajo el tratamiento con melatonina por 14 días.


Subject(s)
Animals , Rats , Melatonin/pharmacology , Tibial Fractures/drug therapy , Tibia/drug effects , Disease Models, Animal , Melatonin/administration & dosage , Osteonectin/drug effects , Osteonectin/metabolism , Osteopontin/drug effects , Osteopontin/metabolism , Rats, Sprague-Dawley , Tibial Fractures/pathology , Tibia/pathology , Wound Healing/drug effects
18.
Braz. j. med. biol. res ; 49(3): e5086, Mar. 2016. tab, graf
Article in English | LILACS | ID: lil-771934

ABSTRACT

High-impact exercise has been considered an important method for treating bone loss in osteopenic experimental models. In this study, we investigated the effects of osteopenia caused by inactivity in femora and tibiae of rats subjected to jump training using the rat tail suspension model. Eight-week-old female Wistar rats were divided into five groups (n=10 each group): jump training for 2 weeks before suspension and training during 3 weeks of suspension; jump training for 2 weeks before suspension; jump training only during suspension; suspension without any training; and a control group. The exercise protocol consisted of 20 jumps/day, 5 days/week, with a jump height of 40 cm. The bone mineral density of the femora and tibiae was measured by double energy X-ray absorptiometry and the same bones were evaluated by mechanical tests. Bone microarchitecture was evaluated by scanning electron microscopy. One-way ANOVA was used to compare groups. Significance was determined as P<0.05. Regarding bone mineral density, mechanical properties and bone microarchitecture, the beneficial effects were greater in the bones of animals subjected to pre-suspension training and subsequently to training during suspension, compared with the bones of animals subjected to pre-suspension training or to training during suspension. Our results indicate that a period of high impact exercise prior to tail suspension in rats can prevent the installation of osteopenia if there is also training during the tail suspension.


Subject(s)
Animals , Female , Bone Diseases, Metabolic/prevention & control , Hindlimb Suspension/physiology , Physical Conditioning, Animal/physiology , Biomechanical Phenomena/physiology , Bone Density/physiology , Cancellous Bone/pathology , Femur/pathology , Femur/physiology , Hindlimb Suspension/adverse effects , Models, Animal , Rats, Wistar , Tibia/pathology , Tibia/physiology
19.
Einstein (Säo Paulo) ; 13(4): 574-579, Oct.-Dec. 2015. graf
Article in Portuguese | LILACS | ID: lil-770488

ABSTRACT

ABSTRACT Objective To analyze the effects of physical exercise on cartilage histomorphometry in osteoporosis-induced rats subjected to immobilization. Methods We used 36 Wistar rats that were separated into six groups: G1, G2 and G3 submitted to pseudo-oophorectomy, and G4, G5 and G6 submitted to oophorectomy. After 60 days at rest, G2, G3, G5 and G6 had the right hind limbs immobilized for 15 days, followed by the same period in remobilization, being free in the box to G2 and G5, and climb ladder to G3 and G6. At the end of the experiment, the rats were euthanized, their tibias bilaterally removed and submitted to histological routine. Results There was significant increase in thickness of the articular cartilage (F(5;29)=13.88; p<0.0001) and epiphyseal plate (F(5;29)=14.72; p<0.0001) as the number of chondrocytes (F(5;29)=5.11; p=0.0021) in ovariectomized rats, immobilized and submitted to exercise. In the morphological analysis, degeneration of articular cartilage with subchondral bone exposure, loss of cellular organization, discontinuity of tidemark, presence of cracks and flocculation in ovariectomized, immobilized and free remobilization rats were found. In ovariectomized and immobilized remobilization ladder rats, signs of repair of the cartilaginous structures in the presence of clones, pannus, subcortical blood vessel invasion in the calcified zone, increasing the amount of isogenous groups and thickness of the calcified zone were observed. Conclusion Exercise climb ladder was effective in cartilaginous tissue recovery process damaged by immobilization, in model of osteoporosis by ovariectomy in rats.


RESUMO Objetivo Analisar os efeitos do exercício físico sobre a histomorfometria da cartilagem de ratas induzidas à osteoporose e submetidas à imobilização. Métodos Foram utilizadas 36 ratas Wistar separadas em seis grupos: G1, G2 e G3 submetidas à pseudo-ooforectomia e G4, G5 e G6, à ooforectomia. Após 60 dias em repouso, G2, G3, G5 e G6 tiveram o membro posterior direito imobilizado por 15 dias, seguido pelo mesmo período em remobilização, sendo livres na caixa para G2 e G5, e de subida em escada para G3 e G6. Ao final do experimento, as ratas foram eutanasiadas, e suas tíbias foram retiradas bilateralmente e submetidas à rotina histológica. Resultados Houve aumento significativo de espessura da cartilagem articular (F(5;29)=13,88; p<0,0001) e da placa epifisária (F(5;29)=14,72; p<0,0001), bem como do número de condrócitos (F(5;29)=5,11; p<0,0021) em ratas ooforectomizadas, imobilizadas e submetidas ao exercício em escada. Nas análises morfológicas, verificaram-se degeneração da cartilagem articular com exposição de osso subcondral, perda da organização celular, descontinuidade da tidemark, presença de fissuras e floculações em ratas ooforectomizadas, imobilizadas e com remobilização livre. Nas ratas ooforectomizadas, imobilizadas e com remobilização em escada, observaram-se sinais de reparação das estruturas cartilaginosas com presença de clones, pannus, invasão de vasos sanguíneos subcorticais na zona calcificada, aumento da quantidade de grupos isógenos e espessura da zona calcificada. Conclusão O exercício físico de subida em escada mostrou-se efetivo no processo de recuperação do tecido cartilaginoso danificado pela imobilização, em modelo de osteoporose por ooforectomia em ratas.


Subject(s)
Animals , Female , Cartilage, Articular/pathology , Exercise Therapy/methods , Immobilization/adverse effects , Motor Activity/physiology , Osteoporosis/therapy , Tibia/pathology , Cell Count , Cartilage, Articular/metabolism , Chondrocytes/pathology , Disease Models, Animal , Growth Plate/metabolism , Ovariectomy , Osteoporosis/etiology , Osteoporosis/pathology , Rats, Wistar
20.
Acta ortop. mex ; 29(3): 182-185, ilus
Article in Spanish | LILACS | ID: lil-773381

ABSTRACT

Objetivo: Reportar la capacidad de la proteína morfogenética de lograr la consolidación ósea en pacientes con seudoartrosis congénita de tibia. Pacientes y métodos: Se realizó en tres pacientes con diagnóstico de seudoartrosis congénita de tibia, se hizo seguimiento durante seis meses a cada uno observando la consolidación y capacidad funcional de la extremidad. Resultados: Se logró consolidación en todos los casos presentándose ésta a los tres meses y medio en promedio. Conclusiones: La proteína morfogenética tiene utilidad como coadyuvante para conseguir la regeneración y consolidación ósea en pacientes con seudoartrosis congénita de tibia.


Objective: To report the ability of bone morphogenetic protein to achieve bone healing in patients with congenital pseudoarthrosis of the tibia. Patients and methods: Three patients with a diagnosis of congenital pseudoarthrosis of the tibia were followed-up for six months. Bone healing and functional capacity of the limb were observed. Results: Bone healing occurred in all cases at a mean of three and half months of treatment. Conclusions: Morphogenetic protein is useful as an adjuvant to achieve bone regeneration and healing in patients with congenital pseudoarthrosis of the tibia.


Subject(s)
Child , Child, Preschool , Humans , Male , Bone Morphogenetic Proteins/administration & dosage , Bone Regeneration/drug effects , Pseudarthrosis/congenital , Follow-Up Studies , Pseudarthrosis/pathology , Pseudarthrosis/therapy , Treatment Outcome , Tibia/pathology
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