A new flavonone from seeds of Alpinia katsumadai and its neuroprotective effect on PC12 cells / 中国中药杂志

A new flavonone, named as (2R, 3S)-pinobanksin-3-cinnamate(1), together with six known compounds, pinocem-brin (2), pinobanksin (3), 3-O-acetylpinobanksin (4), galangin (5), kumatakenin(6), and 3-methylkaempferol (7), were isolated from a 95% ethanol extract of seeds of Alpinia katsumadai through a combination of various chromatographic techniques, including silica gel and Sephadex LH-20. The structure of compound 1 was elucidated by spectroscopic data analysis. Compound 1 exhibits a potent neuroprotective effect against the corticosterone-damaged PC12 cells, which may be underlying the effect by scavenging intracellular ROS.

Research progress on pharmacology, pharmacokinetics and determination of ergosta-4,6,8 (14),22-tetraen-3-one / 中国中药杂志

Ergosta-4,6,8(14),22-tetraen-3-one (ergone) is one of main components in many medicinal fungi. Ergone has been reported to possess the activities of diuresis, cytotoxicity, antitumor, immunosuppression, as well as treatment of chronic kidney disease. According to reported literatures, an overview of spectroscopy characteristics, content determination, pharmacological activity and pharmacokinetics, etc. for ergone is presented in this review. Furthermore, the present review can provide a certain reference value for the further study and development of ergone.

A new steroid with long cross-conjugation structure from the marine-derived fungus Aspergillus aculeatus / 药学学报

A new steroid with a long cross-conjugation structure, 15a-hydroxy-(22E, 24R)-ergosta-3, 5, 8 (14), 22-tetraen-7-one (1), was isolated from the marine-derived fungus Aspergillus aculeatus. Its structure was established by the extensive spectroscopic analyses, and its cytotoxicities against P388, HL-60, and PC-3 cell lines were measured in vitro.

Alisol B inhibited complement 3a-induced human renal tubular epithelial to mesenchymal transition / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study whether alisol B could inhibit complement 3a (C3a) induced renal tubular epithelial-mesenchymal transition (EMT).</p><p><b>METHODS</b>The in vitro cultured human renal tubular epithelial HK-2 cells were intervened with 5 ng/mL transforming growth factor-beta (TGF-beta), 0.1 micromol C3a, and 0.1 micromol C3a + 10 micromol alisol B, respectively. The mRNA and protein expressions of alpha-SMA, E-cadherin, and C3 were detected using RT-PCR, Western blot, and immunofluorescence, respectively.</p><p><b>RESULTS</b>The mRNA and protein expressions of C3 in HK-2 cells were up-regulated after intervention of C3a (P < 0.01), the mRNA and protein expressions of alpha-SMA in HK-2 cells were obviously enhanced (P < 0.01), the mRNA and protein expressions of E-cadherin obviously decreased (P < 0.01). When compared with the group intervened by exogenous C3a, after intervention of alisol B, the mRNA and protein expressions of alpha-SMA in HK-2 cells were obviously reduced (P < 0.01), the mRNA and protein expressions of E-cadherin obviously increased (P < 0.05).</p><p><b>CONCLUSIONS</b>Exogenous C3a could induce renal tubular EMT. Alisol B was capable of suppressing C3a induced EMT.</p>

Chemical constituents from Sarcandra glabra / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of the plant of Sarcandra glabra and provide reference for the study of the bioactive substances.</p><p><b>METHOD</b>The compounds were isolated from the EtOH extract by various chromatographic methods and their structures were elucidated by their physico-chemical properties and the analysis of their spectral data.</p><p><b>RESULT</b>Nine compounds were isolated and identified as isoscopletin (1), syringaresinol monoside (2), styraxjaponoside B (3), 5-O-caffeoylshikimic acid (4), shizukanolide E (5), isoastilbin (6), neoisoastilbin (7), astilbin (8), neoastilbin (9).</p><p><b>CONCLUSION</b>Compounds 1-7 were isolated from S. glabra for the first time.</p>

Simultaneous determination of four alisols in Rhizoma Alismatis by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of alisol A, alisol F, alisol A 24-acetate and alisol B 23-acetate in the rhizome of Alisma orientalis.</p><p><b>METHOD</b>The separation was performed on a Shim-Pack CLC-ODS a C18 column (6 mm x 150 mm, 5 microm) eluted with the mobile phases of acetonitrile (A)-water (B) in gradient elution. The absorbance was monitored at 210 nm. Orthogonal test was adopted to optimize the extraction conditions of the four alisols.</p><p><b>RESULT</b>The correlation coefficients of the four alisols were higher than 0.999 and the average recoveries were 99.23%, 96.67%, 97.30% and 99.61%, respectively. All the RSDs were less than 3%.</p><p><b>CONCLUSION</b>The validation data demonstrated that the method was accurate and repeatable, and can be used to measure the four alisols in Rhizoma Alismatis.</p>

Variation in yield and quality of Alisma orientalis grown under different ecological climatic regions / 中国中药杂志

<p><b>UNLABELLED</b>To compare yield, alisol content of Alisma orientalis planted at different ecological climatic regions, and explore further the impact of environmental factors on the yield and quality.</p><p><b>METHOD</b>Different local varieties were planted at varing ecological climatic conditions. Diameter, yield was measured after harvest, the contents of 23-acetyl alisol B and 24-acetyl alisol A were quantitatively analyzed by HPLC.</p><p><b>RESULT</b>The result revealed that ecological condition had significant impacts on yield and alisol content. Yield of MeiShan was the highest which was up to 1 200.72 kg x hm(-2). The contents of 23-acetyl alisol B and 24-acetyl alisol A of A. orientalis cultivated in Dujiangyan were significantly higher than those of other regions, the values were up to 4.222, 2.727 g x kg(-1), respectively. 23-acetyl alisol B content was positively correlated with 24-acetyl alisol A content (P < 0.01). The diameter was positively correlated with yield (P < 0.01).</p><p><b>CONCLUSION</b>Considering yield and medicinal ingredients, Dujiangyan may be the most suitable region to plant A. orientalis.</p>

Studies on chemical components of Lobelia chinensis / 中国中药杂志

<p><b>OBJECTIVE</b>To study on the chemical constituents of Lobelia chinensis.</p><p><b>METHOD</b>The coloumn chromatographic techniques were applied to isolate constituents, and their structures were elucidated by means of spectral data analysis.</p><p><b>RESULT</b>Sixteen compounds were isolated and identified as daucosterol (1), diosmetin (2), apigenin (3), chrysoeriol (4), loteolin (5), hesperidin (6), loteolin-7-O-beta-D-glucoside (7), apigenin-7-O-beta-D-glucoside (8), linarin (9), diosmin(10), 5,7-dimethoxy-8- hydroxycoumarin (11), palmitinic acid (12), lacceroic acid (13), stearic acid (14), beta-sitosterol (15), daucosterol (16).</p><p><b>CONCLUSION</b>All of these compouds were obtained from L. chinensis for the first time.</p>

Chemical constituents from involatile moiety of Pogostemon cablin / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of involatile moiety of Pogostemon cablin.</p><p><b>METHOD</b>Compounds were isolated and purified by repeated column chromatography, and their structures were elucidated by spectroscopic analysis.</p><p><b>RESULT</b>Nine compounds have been isolated and identified: epifriedelinol (1), 5-hydroxymethol-2-furfural (2), succinic acid (3), beta-sitosterol (4), daucosterol (5), crenatoside (6), 3'''-O-methylcrenatoside (7), isocrenatoside (8), and apigenin-7-O-beta-D-(6"-p-coumaryl)-glucoside (9).</p><p><b>CONCLUSION</b>Compounds 2, 3, 6-8 were isolated from Pogostemon genus for the first time.</p>

Chemical constituents in roots of Osbeckia opipara / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of the roots of Osbeckia opipara.</p><p><b>METHOD</b>Repeated column chromatography over silica gel, RP-18 and Sephadex LH-20, and preparative thin layer chromatography(PTLC) were used to isolate the compounds, whose structures were determined by spectroscopic methods by direct comparing spectral data with those reported references.</p><p><b>RESULT</b>From the MeOH extract of the roots O. opipara, twelve compounds were isolated and identified as follows: lasiodiplodin (1) , de-O-methyllasiodiplodin (2), 2, 3- dihydro-2-hydroxy-2, 4-dimethyl-5-trans-propenylfuran-3-one (3), integracin (4), 5alpha, 8alpha-epidioxy-(22E, 24R)-ergosta-6, 22-dien-3beta-ol (5), 3, 3', 4'-tri-O-methylellagic acid (6), 5-hydroxymethyl furaldehyde (7), vomifolio (8) , betulintic acid (9), 2alpha-hydroxyursolic acid (10), (24R)-stigmast-4-ene-3-one (11), and eugenitin (12).</p><p><b>CONCLUSION</b>Compounds 1-12 were isolated from O. opipara for the first time.</p>

Chemical constituents of Alisma orientalis and their immunosuppressive function / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents with immunosuppressive function from Alisma orientalis.</p><p><b>METHOD</b>The chemical constituents were isolated and purified by kinds of column chromatography and its structures were elucidated by NMR spectra and physicochemical properties. Its immunocompentence of lymphocytes taken from spleen of mouse were examined by MTT assay.</p><p><b>RESULT</b>Twelve compounds were isolated and identified as clovandiol (1), orientalol E (2), alismoxide (3), alismol (4), 4alpha, l0alpha-dihydroxy-5beta-H-guaj-6-en (5), alismorientols A (6), alisol F (7), alisol A (8), 13beta,17beta-epoxy alisol A (9), alisol B 23-acetate (10), 1H-indole-3-carboxylic acid (11) and cuccinic acid (12). Compounds 9, 10 and alisol A 24-acetate showed immunosuppressive function.</p><p><b>CONCLUSION</b>Compounds 1, 5, 11 and 12 were isolated firstly from this genus,and the NMR spectra data of 1 were corrected firstly, some protostan-type triterpenoids may be developed as new drug with immunosuppressive function.</p>

Studies on phenolic compounds from Sophora alopecuroides / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Sophora alopecuroides.</p><p><b>METHOD</b>The non-alkaloid constituents on 95% ethanol extracts of S. alopecuroides were separated and purified with various chromatographic method after solvent extraction and their structures were elucidated by means of physical property and the analysis of their spectral data.</p><p><b>RESULT</b>Seven compounds were isolated and identified as ferulic acid (1), butein (2), 7,3',4'-trihydroxyursaflavonoid (3), 7-hydroxy-3', 4'-methylenedioxyisoflavone (4), butein4-O-beta-D-glucopyranoside (5), piscidic acid (6) and 7,3',4'-trihydroxyur-sadimethoxyflavan-7-O-beta-D-glucopyranoside (7).</p><p><b>CONCLUSION</b>Seven compounds were obtained from this plant for the first time.</p>

Effects of S-3307 on the yield and main ingredients of Alisma plantago-aquatica / 中国中药杂志

<p><b>UNLABELLED</b>To study the effect of S-3307 on the yield and main ingredients of Alisma plantago-aquatica.</p><p><b>METHOD</b>The contents of 24-acetyl alisol A and the 23-acetyl alisol B in tuber were determined by HPLC.</p><p><b>RESULTS</b>The contents of 24-acetyl alisol A and the 23-acetyl alisol B as well as yield were significantly increased in all groups applied with different concentrations of S-3307 comparing with control group. The optimal concentration of S-3307 was 80 mg x kg(-1). The residues of S-3307 was detected under 0.316 8 mg x kg(-1) (detecting limit).</p><p><b>CONCLUSION</b>The optimal concentration of S-3307 is 80 mg x kg(-1), it reached the best result when applied 36 d after seedling.</p>

Studies on chemical constituents of Cynanchum forrestii / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Cynanchum forrestii.</p><p><b>METHOD</b>Chromatographic techniques were applied to isolated chemical constituents. The structures were identified on the basis of physico-chemical constants and spectroscopic data.</p><p><b>RESULT</b>Eight compounds were isolated from the 95% ethanol extract of the roots of C. forrestii and elucidated as ( + ) -5'-methoxyisolariciresinol 3a-O-beta-D-glucopyranoside (1), hexahydroxycholest-7-en-6-one (2), tylophorinidine (3), sucrose (4), palmitic acid (5), beta-sitosterol (6), daucosterol (7), nonanedioic acid (8).</p><p><b>CONCLUSION</b>Compounds 1-3 from this genus, and compounds 4-8 from the plant were obtained for the first time.</p>

Establishment of chromatographic fingerprint and quality assessment on Sichuan native medicinal plant Alisma plantago-aquatica / 中国中药杂志

<p><b>OBJECTIVE</b>To establish chromatographic fingerprint of Sichuan native medicinal plant Alisma plantago-aquatica by RP-HPLC for the quality control.</p><p><b>METHOD</b>The gradient elution mode was applied in chromatographic separation, and data were analyzed by "Computer Aided Similarity Evaluation" software to compare the quality of A. plantago-aquatica samples from different habitats.</p><p><b>RESULT</b>The HPLC chromatographic fingerprinting of A. plantago-aquatica with 26 characteristic peaks was established from 17 lots of A. plantago-aquatica samples, peak 16 and 22 were identified as 24-acetyl alisol A and 23-acetyl alisol B, respectively.</p><p><b>CONCLUSION</b>The chromatographic fingerprinting of A. plantago-aquatica with high specificity can be used to control its quality and assure lot to lot consistency. The RP-HPLC fingerprint method is repeatable, feasible in analysis of A. plantago-aquatica.</p>

Studies on the chemical constituents in herb of Ranunculus sceleratus / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and determine the chemical constituents from Ranunculus sceleratus.</p><p><b>METHOD</b>Six compounds were isolated and purified by extraction and different kinds of column chromatography. The structures were determined on the basis of extensive spectral analysis such as EI-MS, 1H-NMR, 13C-NMR, DEPT and NOEDS.</p><p><b>RESULT</b>The structures were elucidated as stigmasta-4-ene-3,6-dione I, stigmasterol II, isoscopoletin III, scoparone IV, protocatechuic aldehyde V and protocatechuic acid VI, respectively.</p><p><b>CONCLUSION</b>Compound I, III, IV, V and VI were isolated from R. genus for the first time.</p>

Studies on chemical constituents in the mycelia from fermented culture of Flammulina velutipes / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the mycelia of Flammulina velutipes.</p><p><b>METHOD</b>The compounds were isolated with silica gel column chromatography and their structures were elucidated on the basis of chemical evidences and spectral analysis (IR, EI-MS, 1H-NMR, 13C-NMR).</p><p><b>RESULT</b>Five compounds were identified as 5alpha,8alpha-epidioxy-(22E,24R)-ergost-6,22-dien-3beta-ol (1), ergosta-4,6,8(14),22-tetraen-3-one (2), sterpuric acid (3), mannitol (4), ribitol (5).</p><p><b>CONCLUSION</b>The compounds (2)-(5) were isolated for the first time from the mycelia of Flammulina velutipes.</p>