Simultaneous determination and pharmacokinetics of five rhubarb anthraquinones in dog plasma by HPLC after orally administration the rhubarb extract

Rhubarb is widely used in the treatment of obstipation, gastrointestinal indigestion and other diseases in China and other Asian countries for thousands of years. Anthraquinones are the major group of polyphenol constituents including aloe-emodin, rhein, emodin, chrysophanol and physcion. In order to evaluate the pharmacokinetics of five rhubarb anthraquinones, a high-performance liquid chromatography with fluorescence detection [HPLC-FLD] method for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in dog plasma was established. Solid phase extraction [SPE] was applied to the extraction and purification of samples. The calibration curves of five anthraquinones showed good linearity with r greater than 0.9925. The average extraction recoveries, examined at three concentration levels, carried from 92.1% to 102.3%, and the accuracies ranged from 87.7% to 102.5% with precision [RSD] <10%. The pharmacokinetic paremeters of five anthraquinones were investigated systematically after orally administration the rhubarb extract. Five anthraquinones were rapidly absorbed and T[max] for aloe-emodin, rhein, emodin, chrysophanol and physcion was at 0.75, 1.50, 0.75, 1.0 and 2.0 h respectively. The C[max] of five anthraquinones was 0.031, 3.39, 0.27, 0.036 and 0.032 micro g/mL while the AUC of five anthraquinones was 0.35 +/- 0.058, 32.22 +/- 8.29, 2.97 +/- 0.66, 0.43 +/- 0.10 and 0.41 +/- 0.12 mg h/L, respectively

Antitumor mechanism of Qinghaosu derivatives--molecular docking studies of Qinghaosu derivatives with transferrin / 药学学报

To investigate the antitumor mechanism of artemisninin, a flexible docking analysis was used to score all kinds of functions of 11 Qinghaosu derivatives and transferrin with different resolutions. The distances of Asp-63, Tyr-188, His-249, Arg-124 and Lys-296 with Qinghaosu were less than 0.5 nm, separately. Meanwhile, the higher is the activity of Qinghaosu derivatives the higher is the score. Our model explains that Fe2+ is more feasible to react with Qinghaosu, and not involved in other metabolism in presence of transferrin. Docking results unveil that Iron(II)-transferrin increased the cytotoxicity of Qinghaosu derivatives and provide a rational basis for further design and synthesis of novel Qinghaosu derivatives.

Phosphorylated modification of genistein and their interaction with lysozyme / 药学学报

A series of genistein's phosphates were synthesized through a simplified Atherton-Todd reaction and the structures of the phosphates were determined by electrospray ionization mass spectrometry (ESI-MS) and NMR. In case of monophosporyl derivatives, NMR spectra show that substitutions occur at the 7-position of genistein but not at its 4' and 5-position. Moreover, the non-covalent complexes of lysozyme with the four new genistein phosphates were detected by ESI-MS.

Interaction of bovine serum albumin with luteolin and apigenin / 药学学报

<p><b>AIM</b>To study the interaction mechanism of bovine serum albumin (BSA) with luteolin and apigenin.</p><p><b>METHODS</b>Fluorescence quenching method and non-radioactive energy transfer theory were used.</p><p><b>RESULTS</b>The binding constants at different temperature were determined and the quenching mechanism of them were suggested as static quenching. The transfer efficiency of energy and distance between BSA and luteolin or apigenin were investigated according to the mechanism of the Förster energy transference.</p><p><b>CONCLUSION</b>The interaction between them seems to be strong and the binding force were mainly hydrophobic force. B(3')-OH,B(4')-OH strengthened the interaction of flavonoids and BSA.</p>

A quantum chemistry investigation on antimalarial mechanism of Qinghaosu based on cleavage of the peroxide bridge / 药学学报

<p><b>AIM</b>To investigate antimalarial mechanism of Qinghaosu ( QHS) and its derivatives.</p><p><b>METHODS</b>The electronic structure of QHS and its derivatives were completely optimized and calculated at B3LYP/6-31G * level, while the route was at HF/STO-3G level.</p><p><b>RESULTS</b>The peroxide bridge is the active center of QHS and induced by ferrous iron to produce cyclic product.</p><p><b>CONCLUSION</b>Heme can link with QHS derivatives.</p>

Determination of SARS-CoV by simplified nested fluorescent RT-PCR / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To establish a simple rapid and sensitive nested RT-PCR method for detection of SARS coronavirus RNA by designing the specific primers for SARS and optimizing the parameters for PCR.</p><p><b>METHODS</b>Primers and fluorescent probes were designed according to the sequences of SARS coronavirus genes available from GenBank. The optimization of the parameters for PCR was performed in PE 7700 thermal cycle. The 36 serum samples and 40 mouthwash of SARS patients and 80 samples of healthy people were tested.</p><p><b>RESULTS</b>The positive rate of patient serum and mouthwash was 33.6%, (12/36) and 67.5%, (27/40), respectively, while the positive rate of healthy people was zero (0/160).</p><p><b>CONCLUSION</b>The simple nested RT-PCR method was a rapid, efficient and sensitive one for SARS early diagnosis.</p>

Coxsackie virus B types were discriminated by RT-PCR / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.</p><p><b>METHODS</b>A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.</p><p><b>RESULTS</b>This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.</p><p><b>CONCLUSION</b>The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.</p>

Synthesis and antibacterial activity of 7-(4-acylamino-thiocarbamoyl-1-piperazinyl) fluoroquinolone analogues in vitro / 药学学报

<p><b>AIM</b>To synthesize new fluoroquinolone analogues as antibacterial compounds.</p><p><b>METHODS AND RESULTS</b>By reaction of acryl chloride(chloro-carbonic ester) with sodium sulfocyanate, acyl isosulfocyanic ester were easily obtained. Twelve 7-(4-acylamino-thiocarbamoyl-1-piperazinyl) fluoroquinolone analogues (1-12) were synthesized through modifying the 7-piperazine of norflorxacin and ciprofloxacin with isosulfocyanic ester synthesized above. The structures of synthesized compounds were characterized by 1HNMR, IR and elemental analysis.</p><p><b>CONCLUSION</b>Antibacterial activities of the new compounds were evaluated in vitro compared with norflorxacin. Compounds 5, 7, 10 and 12 showed antibacterial activities.</p>