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Objective:To observe the effects of fuyuan capsule on change of morphology of synovium,expression of IL-1?、MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits,and investigate the mechanism about the reparation and treatment of fuyuan capsule.Methods:Sixty New Zealand rabbits were divided into five groups randomly:normol group,model contrast group,low dose group, middle dose group and high dose group of fuyuan capsule.OA model was induced in rabbits by improved Hulth'S method.Three Chinese herb groups were treated with fuyuan capsule(0.727,1.453,4.795 g(/kg?d)),continuously for 4,8 weeks and 12 weeks,model contrast group was treated with 0.9%NS,ig,at the same time.Synovium sections were analyzed by immunohistochemistry for the IL-1?,MMP-13 and iNOS,observation and measurement of the change of morphology of synovium by light microscopic and transmission electron microscopic.After the experiment,the differences of these indexes between Three Chinese herb groups and model contrast group were compared.Results:There are found significant differences about measurement of morphology of synovium between Three Chinese herb groups and model contrast group after the experiment(P
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Objective: To observe the acute toxicity and the long-period toxicity reaction of Fuyuan Capsule(FYC) to provide the reference dosage for human beings. Methods:Tests of acute oral toxicity: 40 mice (Kun Ming kind) were randomly divided into two groups,the drug test group and the control group. The maximum administration dosage(MAD) of FYC were determined by iq administration in mice. Tests of long oral toxicity:80 SD rats were randomly divided into four groups:high,middle,and low dose of FYC group and control group.FYC was continuously administered for 90 days.Rats were given drugs every day. When the experiment was finished and withdrawed for 14 days,rats were killed and the growth condition,blood cell counting and biochemical targets were measured.Histomorphologieal examination were simultaneously performed. Results:The MAD of FYC by iq administration was greater than 30g/kg in mice.In the long-period toxicity test,after administered repeatedly for 90 days,in high dose group AST,ALT and Crea increased(P
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Objective:To explore the influence of fuyuan capsule treatment on patients with qi deficiency and blood stasis. Methods:40 cases with qi deficiency and blood stasis were divided into two groups,the fuyuan capsule group and the control group. Besides basic diseases were treated in two groups, the patients in control group were treated with xinxuekang capsule, while patients in fuyuan capsule group were treated with fuyuan capsule instead of xinxuekang capsule. Scores of symptom and indexes of Hemorheology and heart function were tested after 8 weeks.The data were compared with those of pretherapy. Results:Scores of symptom and indexes of Hemorheology in the fuyuan capsule group were significantly lower than those in the control group(P
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Objective:To study the effects of FuYuan capsule on articular cartilage degradation and expression of interleukin beta 1 and transforming growth factor beta 2 in osteoarthritis(OA).Methods:Fifty-four healthy New Zealand rabbits received unilateral anterior cruciate ligament transaction(ACTL)to establish model of OA.One week after operation they were randomly divided into 4 groups,named as model control group and FuYuan capsule low,moderate and high dose groups with 12 rabbits in each group.The other 6 rabbits divided into normal control and model control group were given physiological saline.Treatment groups were perfused with FuYuan capsule(727mg/kg、1453mg/kg、4795mg/kg)once a day for 4,8 and 12 weeks.Samples of joint were collected at the 4th,8th,and 12th week after managements for general,light microscopic and electron microcope observation,and the distribution of IL-1?and TGF?2 of cartilage was documented by immunohistochemistry.Cartilage of medial femoral condyles and tibial plateau at the margin of lesion nearby were obtained and mRNA expression of TGF?2 was detected using reverse transcription-polymerase chain reaction(RT-PCR).Results:Cartilage degeneration in FuYuan capsule group was significantly slighter than that in model control group.The levels of IL-1? in treatment groups were lower significantly than those in model control group(P
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OBJECTIVE:To explore the effect of Fuyuan capsule on the mRNA expression of HIF-1? in kidney of sepsis rats. METHODS:Male SD rats were randomly divided into sham group,model group and Fuyuan capsule group (low dose and high dose) with 4 rats in each group. CLP operation was done in Fuyuan capsule groups after four days of intragastric administration. RT-PCR testing was applied to determined the expression of HIF-1a in 3 h,6 h,12 h and 24 h after model induction. The level of IL-6 was measured by ELISA method. Blood of heart was sampled for detection of renal function. HE staining was used to observe pathological change of kidney. RESULTS:As compared with sham group,the mRNA expression of HIF-1? increased gradually within 3 h and reached peak at 24 h(P
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Objective To observe the effect of Fuyuan Capsule (FC) on vascular endothelial cell function of rats with qi deficiency and blood stasis.Method Forty-eight 16-month-old SD rats were equally randomized to 5 groups:FC group,positive control group (Qishen Capsule),model group and blank control group.Rat models of qi deficiency and blood stasis were induced by exertion,starvation and cold.The mice were given the drugs by gastric perfusion for 4 weeks.After treatment,endothelin (ET),6-keto-prostaglandin F_(1a) and nitric oxide (NO) levels were mea- sured.Results NO and 6-keto-PGF_(1a) levels were lower in the model group than those in the blank control group (P
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Objective:To study the effects of FuYuan capsule on rat liver fibrosis induced by pig serum.Methods:The rats were randomly divided into six groups,including the normal group,the model group,the low,middle and high dose FuYuan capsule group,and the positive control group(compound Biejiaruangan-chip solution).The rat models of immune liver fibrosis were induced with pig serum.Alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum were detected with autoumatic biochemical analyzer.Hyaluronan(HA),laminin(LN).IV collagen(IV-C)and the typeⅢcollagen(PCⅢ)in serum were quantified by radiative-immune technology.The expression of TIMP-1(tissue inhibitor of metalloproteinases-1)and TGF-?1(transforming growth factor?-1)in the liver were observed by immunohistochemical assay.Results:ALT,AST,HA,LN,IV-C and PCⅢin the model group were significantly higher than those in the other group(sP