A long-term follow up of surgical treatment for a child with melorheostosis: a case report and literature review / 中华骨科杂志

Melorheostosis is a rare disorder of osteopathia. The clinical characteristics of melorheostosis in children is totally different from that in adults. The radiographic features of melorheostosis include the hyperostosis in endosteal on the one side of the cavum medullare and formed streakiness. The soft tissue contracture of the limb and joint deformities are the symptoms of melorheostosis. Here, the authors reported a melorheostosis case of 6 years old girl who suffered from severe valgus deformity of the right knee with permanent patellar dislocation. A surgical stabilization was applied with lateral soft tissue release, medial soft tissue stabilization and transferred the vastus medialis laterally (kinetic stability). After 19 years follow up postoperatively, the limb developed well in satisfied alignment with good function of knee joints, even participated in some sports activities. Other authors reported a similar case of melorheostosis with surgical treatment and achieved good outcomes in limb realignment and reduction. The further suggested that the surgical treatment should be produced before epiphyseal closure.

The comparison of enhanced CT and color ultrasonography to the diagnosis of acute pancreatitis / 中华急诊医学杂志

Objective To study reliable evidence for the diagnosis of acute pancreatitis by comparing the diagnostic potential of enhanced computed tomography (CT) with color ultrasonography.Methods A total of 106 patients with acute pancreatitis admitted from January 2010 to December 2012 were examined by enhanced CT or color Ultrasonography for comparing the diagnostic sensitivity of the two methods.Results A total of 66 cases with acute pancreatitis were diagnosed by color ultrasound with 62.0％ positive rate,while 99 cases with acute pancreatitis were diagnosed by enhanced CT with 93.0％ positive rate,and thus the difference between them was statistically significant (P =0.03).Of them,22 cases associated with enlargement of the gallbladder,cholecystitis,lithiasis of gallbladder and/or bile duct were detected by color ultrasonography,whereas 43 cases associated with enlargement of the gallbladder,cholecystitis,lithiasis of gallbladder and/or bile duct were detected by enhanced CT,thus the difference between them was significant (P =0.02).Conclusions The diagnosis sensitivity of abdominal enhanced CT for acute pancreatitis was superior to abdominal ultrasonography,and enhanced CT is a ideal tool for the diagnosis of acute pancreatitis.

Digital anatomy of nucleus accumbens in the human brain / 解剖学报

Objective To explore the locating, parameter measurement and 3D display of nucleus accumbens in human brain in terms of digital anatomy .Methods The raw data of the head specimen of a 45-year-old male adult with 0.5mm as the section spacing was collected by using digital milling machine .Three hundreds images of continual cross sections containing brain were chosen and the segmentation of the caudate nucleus , putamen and nucleus accumbens was accomplished with Photoshop CS .The nucleus accumbens on the images of continual coronal section reconstruction were distinguished according to Harvard Medical School ’ s segment method to calculate the volume of nucleus accumbens and collect the correlative location information .The caudate nucleus , putamen and nucleus accumbens were 3D visualize with the software of Amira 3.1.1.Results The nucleus accumbens , the adjoining structure and the lesion target of nucleus accumbens were all clearly visible .The left nucleus accumbens volume was 972.5mm3 , and the right was 830.6mm3 .The 3D coordinate value was the left ( -11.0, 24.4, 1.3) and the right (9.3, 23.9, 1.7).Conclusion The digital anatomy of nucleus accumbens can distinctly display the nucleus accumbens , form and confirm it ’ s volume, location and adjoining area , which is useful to clinician .

The design of plantar pressure distribution monitoring system and preliminary clinical application / 生物医学工程学杂志

Plantar pressure distribution can reflect the force of several key points on foot while standing and walking. A comprehensive understanding of the plantar pressure distribution makes great sense in the following aspects: the understanding of the normal foot biomechanics and function, clinical diagnosis, measurement of disease extent, postoperative efficacy evaluation, and rehabilitation research. A simple plantar pressure measurement device was designed in this study. This paper uses FlexiForce flexible sensor to pickup plantar pressure signal and USB A/D board to do data acquisition. The data are transferred into a laptop and processed by a VB-based software which can display, remember and replay the data. We chose patients with hallux valgus and normal people to measure the pressure distribution and make contrast analysis of plantar pressure with this device. It can be concluded that people with hallux valgus have higher pressure on the second metatarsophalangeal joint and the distribution move outward. The plantar pressure of patients postoperative could be greatly improved compared to the preoperative. The function of this device has been confirmed.

Protective effect of ligustrazine hydrochloride on homocysteine-injured ECV304 cells / 中国中药杂志

<p><b>OBJECTIVE</b>To detect the protective effect of ligustrazine hydrochloride on homocysteine-injured ECV304 cells.</p><p><b>METHOD</b>In the in vitro study, human umbilical vein endothelial cells were selected as objects, with homocysteine as the molding agent, to judge the injury degree by monitoring NOS and NO contents. Based on that, the best homocysteine concentration in ECV304 cells, the best reaction time could be determined, and an endothelial cell injury model was established. After adding ligustrazine hydrochloride, NOS and NO contents in injured endothelial cells were determined to observe the protective effect of ligustrazine hydrochloride.</p><p><b>RESULT</b>It was proved that the optimal concentration of homocysteine on injured ECV304 cell was 1 mmol x L(-1), the best reaction time was 48 h. An injured endothelial cell model was established. At the same time, positive drug nitroglycerin and ligustrazine hydrochloride displayed a protection effect on injured ECV304 cells, NOS and NO formation were significantly increased compared with the model group.</p><p><b>CONCLUSION</b>Ligustrazine hydrochloride has a protective effect on homocysteine-injured ECV304 cells. The model established in this study can be used to screen anti-myocardial ischemia drugs targeting at an endothelial cell protective agent.</p>

Determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extractive of Wendan formula by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To develop a RP-HPLC method for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.</p><p><b>METHOD</b>DIKMA Diamonsil(2)-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phase of acetonitrile-0.1% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL min(-1). The detection wavelength was 237, 283 nm.</p><p><b>RESULT</b>The linear responses ranged from 0.0199-0.1191 microg for liquiritin (r = 0.9997, n = 6), 0.1800-1.0800 microg for naringin (r = 0.9997, n = 5), 0.1455-0.8730 microg for hesperidin (r = 0.9998, n = 6), 0.0393-0.2355 microg for monoammonium glycyrrhizinate (r = 0.9997, n = 6), respectively. The average recoveries were 97.7% with RSD 1.5% for liquiritin, 97.7% with RSD 2.0% for naringin, 97.1% with RSD 2.0% for hesperidin and 98.5% with RSD 1.9% for glycyrrhizic acid, respectively.</p><p><b>CONCLUSION</b>The method is quick, simple and repeatable for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.</p>

Advance in Apoptosis Mechanism on Nerve Cell after Cerebral Ischemia-reperfusion and Neuroprotective Drugs (review) / 中国康复理论与实践

@#Cerebral ischemia-reperfusion results in damage on neuron, leading to genes and proteins related to apoptosis activation. At the same time, generous cytokines released after cerebral ischemia-reperfusion can induce the apoptosis of the neuron. Many current studies have showed that the major damage mechanisms on apoptosis of the neuron are mitochondrion impairment, calcium overload, increased levels of oxygen radicals and so on. The advance research on the mechanism contributes to explore new neuroprotective drugs, and further identify the target and therapeutic effect of drug treatment.

Effects of Morroniside on IL-1β in Rat Cortex with Focal Cerebral Ischemia/Reperfusion / 中国康复理论与实践

@#ObjectiveTo investigate the effects of morroniside on IL-1β in rat cortex with focal cerebral ischemia/reperfusion.MethodsThe animal model was induced by occlusion of middle cerebral artery with suture embolus, ischemia for 30 min, and reperfusion for 72 h in rats. The content of IL-1β was detected with enzyme linked immunosorbent assay(ELISA).ResultsCompared with sham group, the content of IL-1β increased obviously in model rat. Compared with model group, morroniside(30 mg/kg, 90 mg/kg, 270 mg/kg) and colchicine (0.06 mg/kg) decreased the content of IL-1β significantly (P<0.001).ConclusionMorroniside may protect the cortex from inflammatory factor IL-1β.

Effects of Morroniside on Activation of Caspase-3 in Rats with Focal Cerebral Ischemia/Reperfusion / 中国康复理论与实践

@#Objective To investigate the effects of morroniside on activation of caspase-3 in rats with focal cerebral ischemia/reperfusion. MethodsThe model was induced with occlusion of middle cerebral artery with suture embolus, ischemia for 30 min, and reperfusion for 72 h in rats. Vitamin E used for the positive control. The activity of caspase-3 was detected with spectrophotometry. ResultsCompared with sham group, the caspase-3 activity increased obviously in model rat. Compared with model group, Morroniside (30 mg/kg,90 mg/kg,270 mg/kg) decreased the activation of caspase-3 remarkably, which was dose-dependent (P<0.05). ConclusionMorroniside may reduce apoptosis by decreasing the activation of caspase-3 in rats.

Advance in Neurogenesis after Focal Cerebral Ischemia-reperfusion Injury and Effect of Drug (review) / 中国康复理论与实践

@#Neural stem cells (NSCs), which will proliferate and differentiate into neurons and glial cells under certain conditions, involved in the repair of neurological function. This process is called neurogenesis. Focal cerebral ischemia-reperfusion injury is one of the most common diseases, which can induce the neurological functional deficits. It is significant to study the response and regulation of NSCs after focal cerebral ischemia-reperfusion injury. In this article, we reviewed the characteristics, molecular mechanisms, putative endogenous mediators and exogenous stimulators of neurogenesis in adult brain following ischemic injury, and response and regulation of drug in ischemic injury following neurogenesis.

Effects of Morroniside on Rats Neurons in Focal Cerebral Ischemia/Reperfusion Injury / 中国康复理论与实践

@#ObjectiveTo investigate the effects of morroniside on super oxide dismutase (SOD) and neurons in rats cortex with focal cerebral ischemia/reperfusion. MethodsThe animal model was induced by middle cerebral artery occlusion with suture embolus, cerebral ischemia 30 min and reperfusion 3 d or 7 d. Vitamin E for the positive control. The content of SOD was detected with spectrophotometry and the nerve cells was observed with immunohistochemistry. ResultsCompared with model group, morroniside （270 mg/kg）increased the activity of SOD and the number of neurons （30 mg/kg, 90 mg/kg, 270 mg/kg） significantly. ConclusionMorroniside may have neuroprotective effect and increasing the activity of SOD in rats cortex.

Effect of Morroniside on Cerebral Infarction Volume in Focal Cerebral Ischemia-reperfusion Rats / 中国康复理论与实践

@#Objective To observe the effect of morroniside on cerebral infarction volume in focal cerebral ischemia-reperfusion rat model.Methods The animal model was induced by occlusion of middle cerebral artery with suture embolus, ischemia for 30 minutes, and reperfusion for 7 days. The infarction volume was measured by 2,3,5-triphenyltetrazolium chloride(TTC) staining technique.Results Compared with sham operation group, the cerebral infarction volume ratios increased obviously in model group and in the drug-treated（90 mg/kg，270 mg/kg）groups. Compared with model group, the cerebral infarction volume ratios decreased obviously in the morroniside-treated（90 mg/kg，270 mg/kg）group,while the cerebral infarction volume ratios in vitamin E-treated（35 mg/kg）group didn't change.Conclusion Morroniside may decrease the cerebral infarction volume after cerebral ischemia-reperfusion. It possesses protective effect against cerebral ischemia-reperfusion injury.

Advance in Damage Mechanism on Blood Brain Barrier after Cerebral Ischemia-reperfusion and Neuroprotective Drugs (review) / 中国康复理论与实践

@#Cerebral ischemia-reperfusion Results in breakdown on construction and function of blood brain barrier, leading to hemorrhage transformation and brain edema. At the same time, generous cytokines and chemokines released after cerebral ischemia-reperfusion can regulate the opening of the blood brain barrier. Many current studies showed that the major damage mechanisms on blood brain barrier are inflammatory infiltration, proteolysis, opening aquaporin and so on. The deep research on the mechanism contributes to explore new neuroprotective drugs, and further identify the target and therapeutic effect of drug treatment.

Advance in Damage Mechanism on Blood Brain Barrier after Cerebral Ischemia-reperfusion and Neuroprotective Drugs (review) / 中国康复理论与实践

@#Cerebral ischemia-reperfusion Results in breakdown on construction and function of blood brain barrier, leading to hemorrhage transformation and brain edema. At the same time, generous cytokines and chemokines released after cerebral ischemia-reperfusion can regulate the opening of the blood brain barrier. Many current studies showed that the major damage mechanisms on blood brain barrier are inflammatory infiltration, proteolysis, opening aquaporin and so on. The deep research on the mechanism contributes to explore new neuroprotective drugs, and further identify the target and therapeutic effect of drug treatment.

Effects of Morroniside on Total Antioxidative Capacity in Rat Cortex with Focal Cerebral Ischemia/Reperfusion / 中国康复理论与实践

@# Objective To investigate the effects of morroniside on total antioxidative capacity (T-AOC) in rat cortex with focal cerebral ischemia/reperfusion. Methods The animal model was induced with occlusion of middle cerebral artery. The T-AOC was detected with spectrophotometer. Results Compared with model group, morroniside （270 mg/kg） increased the T-AOC significantly. Conclusion Morroniside may take the neuroprotection through increased T-AOC of rat cortex.

Effects of Morroniside on Apoptosis in Rats with Focal Cerebral Ischemia/Reperfusion Injury / 中国康复理论与实践

@#Objective To investigate the effects of morroniside on glutathione (GSH) and Caspase-3 in rats with focal cerebral ischemia/reperfusion. Methods The animal model was induced by middle cerebral artery occlusion with suture embolus with 30 min for cerebral ischemia and 7 d for reperfusion. The content of GSH was detected with spectrophotometry and Caspase-3 expression was observed by Western blot.Results Compared with model group, the GSH increased and Caspase-3 expression reduced significantly at 270 mg/kg of morroniside.Conclusion Morroniside may have neuroprotective effect by increasing GSH in rats cortex and reduce the apoptosis in focal cerebral ischemia/reperfusion injury.

Inflammatory Reaction during Cerebral Ischemia Reperfusion Injury (review) / 中国康复理论与实践

@#The cerebral ischemia reperfusion injury is one of factors in aggravating brain injury, and the inflammatory response is one of the main reasons in the reperfusion injury after acute cerebral ischemia. Inflammation is modulated by many factors such as inflammatory mediators and inflammatory cells that promote brain damage from ischemia injury to reperfusion injury. This paper would review the role of inflammatory cells and mediators such as cytokines, chemotactic factors, adhesion molecules on cerebral tissue injury.

Morroniside Inhibiting Lipid Peroxidation of Cortex in Rats with Focal Cerebral Ischemia/Reperfusion / 中国康复理论与实践

@#Objective To investigate the effects of morroniside on lipid peroxidation of cortex in rats with focal cerebral ischemia/reperfusion. Methods The animal model was induced by occlusion of middle cerebral artery with suture embolus, ischemia for 30 min, and reperfusion for 72 h in rats. The content of malondialdehyde (MDA) was detected with spectrophotometry. Results Compared with sham group, the content of MDA increased obviously in model rat. Compared with model group, Morroniside（30 mg/kg，90 mg/kg，270 mg/kg）and vitamin E（35 mg/kg） decreased the content of MDA significantly (P<0.05). Conclusion Morroniside may protect the cortex from lipid peroxidation.

Repair of rabbit radius defects by inductive artificial periosteum and allogenic bone grafts / 中华骨科杂志

Objective To investigate the feasibility of repairing the radius defect with inductive bone regeneration membrane combined with freezed allogenic bone. Methods Preparation PLGA/bBMP+IGF-Ⅱ+ bFGF complosite was prepared as artificial periosteum. Twenty seven rabbits with 1.0 cm long right radius segmental osteoperiosteum defect were werved as the animal model, and randomly assigned to 3 groups with 9 in eath group; Group A: the defects were connected with the PLGA/bBMP+IGF-Ⅱ+bFGF membrane and allogenic bone; Group B: the defects were treated with the PLGA/bBMP+IGF-Ⅱ+bFGF membrane; Group C: the defects were implanted only woth allogenic bone grafts. Animals were killed at 2, 4, 8 weeks postoperation respectively. The healing was study with radiological, histological and immunological methods. Results The osteogenic ability in PLGA/bBMP+IGF-Ⅱ+bFGF group was superior to that in the other groups. The defects of group A ware fully repaired at week 8; bony bridging under remodeling was observed in group B; and the putrescence of allogenic bone which was packaged by mass of connective tissue was observed with group C; Group A caused lower titer of antibody than group C and inhibited lymphocytes reproduction in vitro; There was significant difference between group A and group C. Conclusion PLGA/bBMP+IGF-Ⅱ+bFGF artificial periosteum had provided a excellent osteoinduction condition. Artificial periosteum combined with freezed allogenic bone can effectively promote the rapair of radius defects of rabbit.

Studies on antishock effect of active constituents in GINSENG SINI TANG / 中草药

Object To study the chemical constituents of the extracts S 1 and S 7 in GINSENG SINI TANG, which has the effect of antihemorrhagic shock Methods The constituents of S 1 and S 7 were isolated and purified by silica gel column chromatographic methods and analyzed by ESI/MS n, MALDI TOF/MS Results The 12 compounds were identified as ginsenosides Ra 1, Ra 2, Rb 1, Rb 2, Rb 3, Rc, Rd, Re, Rg 1, Rg 2, Rg 3, Rf from constituent S 7 in GINSENG SINI TANG The six compounds of diterpenoid alkaloid were identified as 14 benzoylhypaconine 8 linoleate (HAL), 14 benzoyldeoxyaconine 8 oleate (HAO), 14 benzoylhypaconine 8 palmitate (HAP), benzoylmesaconitine (BM), benzoylaconitine (BA), benzoylhypaconitine (BH) from constituent S 1 in GINSENG SINI TANG Conclusion All these compounds were obtained from GINSENG SINI TANG and identified for the first time