Reviews on Historical Evolution and Modern Research of Rhizoma Pinelliae Fermentata / 世界科学技术-中医药现代化

Rhizoma Pinelliae Fermentata(RPF)wasoneofthecommonlyusedChinesemateriamedica(CMM). According to the ancient and modern literatures on RPF, the historical evolution, fermentation methods, chemical compositions, efficacy and microbes of RPF were systematically summarized in this paper. Through the analysis on existing problems of fermentation strains, effective components, quality standard and fermentation process, the corresponding solutions were proposed. This work may provide an idea and reference for the further study of RPF.

Research on the HPLC characteristic fingerprints of Rhizoma Pinelliae and processed products / 中国药学杂志

OBJECTIVE: To establish and compare the HPLC fingerprints of different parts of Rhizoma Pinelliae (RPI), Rhizoma Pinelliae Praeparatum Cum Alumine (RPA), Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine (RPZ), Rhizoma Pinelliae Praeparatum (RPP) and Jing Pinelliae (JPI) and provide reliable method and scientific basis for their quality control. METHODS: The HPLC fingerprints of water, 75% ethanol and 95% ethanol extracts of Rhizoma Pinelliae and processed products were established and analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A). RESULTS: The chromatograms of water, 75% ethanol and 95% ethanol extracts were generated as the representative standard fingerprints. Thirteen common peaks were obtained in the fingerprints of Rhizoma Pinelliae and Rhizoma Pinelliae Praeparatum Cum Alumine; 15 common peaks were obtained in the fingerprints of Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine; 17 common peaks were obtained in the fingerprints of Rhizoma Pinelliae Praeparatum and Jing Pinelliae. Among the common peaks, 8 characterized peaks were identified as inosine, guanosine, adenosine, succinic acid, ephedrine hydrochloride, liquiritin, glycyrrhizic acid, and 6-Gingerol. Guanosine, adenosine, succinic acid, and ephedrine hydrochloride existed in Rhizoma Pinelliae and processed products. 6-gingerol was only detected in Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine. Liquiritin and glycyrrhizic acid were detected in Rhizoma Pinelliae Praeparatum and Jing Pinelliae. A new peak (peak 8) appeared in the chromatograms of Rhizoma Pinelliae Praeparatum Cum Alumine and Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine; Rhizoma Pinelliae Praeparatum and Jing Pinelliae did not show inosine and had a new peak (peak 11) compared to Rhizoma Pinelliae. CONCLUSION: This study established the HPLC characteristic fingerprints of different parts of Rhizoma Pinelliae and processed products. The method is stable, time-saving, reliable and can identify Rhizoma Pinelliae and its four different processed products, which provides a scientific basis for the quality control of Rhizoma Pinelliae.

Determination of Alum Residual Amount in Rhizoma Pinelliae Preparata / 中国中医药信息杂志

Objective To establish a method for the determination of alum residual amount in Rhizoma Pinelliae Preparata from different companies,and to provide a scientific evidence for quality evaluation of Rhizoma Pinelliae Preparata.Methods Na2EDTA titration method was applied for the quantitative analysis.Results There were obvious differences in residual quantity of alum in Rhizoma Pinelliae Preparata from different companies.Conclusion The method is simple,reproducible and accurate,it can be used for the determination of alum residual amount in Rhizoma Pinelliae Preparata.

Clinical experience of veteran TCM practitioner LI Ke on using unprocessed Rhizoma Pinelliae / 中华中医药杂志

Outlining the medicinal properties of unprocessed Rhizoma Pinelliae and its general usage nowadays,this paper highlighs the view of veteran TCM practitioner LI Ke on processed Rhizoma Pinelliae and the reasons for the clinical overdose application and its clinical effect.Besides,before proposing a new thinking to the clinical use of herbs in the future,the author concludes his clinical experience in application of unprocessed Rhizoma Pinelliae under the guidance of Master LI,particularly the application in the treatment of difficulty miscellaneous disease.

Determining of ?-aminobutyric Acid in Fabanxia by Pre-column Derivatization RP-HPLC / 中国药房

OBJECTIVE:To determine the content of ?-aminobutyric acid in Rhizoma Pinelliae Praeparatum.METHO-DS:In alkaline condition,amino acids reacted with phenyl isothiocyanate and resulted in the derivatives of benzene isothiocyanate-amino acid complex.A Venusil-AA amino acid analysis column(250 mm?4.6 mm,5 ?m)was used and the absorbance at 248 nm was measured.The mobile phase A is 0.05 mol?L-1 sodium acetate solution(pH6.5)and the mobile phase B consisted of acetonitrile-water(80∶20).RESULTS:The linear range of ?-aminobutyric acid was from 2.056 ?g?mL-1 to 12.336 ?g?mL-1,and the recovery was 99.0%(RSD=0.92,n=6).CONCLUSION:This method is simple,accurate and reproducible,and it can be used for quality control of Rhizoma Pinelliae Praeparatum.

Study on the Cleaning Action of Rhizoma Pinelliae Mixture on Trachea Secretions / 中国药房

OBJECTIVE:To explore the cleaning action of rhizoma pinelliae mi xture(RPM)on trachea secretions of the model animals.METHODS:The rats were divided randomly into high and low dose of RPM group and the control group;Then the secretion level of phenol red in each group was observed after the forming of models by tracheostomy and intraperitonal injection with phenol red;The respective time that spent on moving cork wood filings to a same distance by oral mucosa ep ?ithelium ciliary movement of toad both before and after medication were observed.RESULTS:The secretion level of phenol red in RPM group increased significantly compared with the control group(P

Purification of a protein with trypsin-inhibiting activity from Rhizoma Pinelliae and analysis of its N-terminal amino acid sequence / 中草药

Objective To purify a Rhizoma Pinelliae protein,determine its inhibiting activity and mechanism to trypsin,and analyze its N-terminal amino acid sequence. Methods Active Rhizoma Pinelliae protein was purified from the 40% (NH_4)_2SO_4 sediment of the crude extracted protein from Rhizoma Pinelliae by affinity chromatography of trypsin-Sepharose CL 4B and gel filtration of Sephadex G-50;12% of SDS-PAGE was used for determining the purity of the purified Rhizoma Pinelliae protein and estimating its molecular weight;N-terminal amino acid sequence of active Rhizoma Pinelliae protein was analyzed by Edman degradation. Results The purified active Rhizoma Pinelliae protein showed a sin-gle band on SDS-PAGE gel,which estimated molecular weight was about 1. 4?10～4,its specific activity was 1 059. 012 U/mg,and the yield was about 24.40%;The N-terminal sequence of the preceding six amino acid residues of active Rhizoma Pinelliae protein was DPVVDG;The quality inhibiting ratio of active Rhi- zoma Pinelliae protein to trypsin was 1:4. 72,and the Ki value ,the inhibition constant was about 7. 17 ?10～(-6) mol/L. Conclusion Active Rhizoma Pinelliae protein is a kind of serine protease inhibitor,a com-petive inhibitor to trypsin.

Quality control of Rhizoma Pinelliae by chromatograph-digitized fingerprint spectrum / 中成药

AIM: To establish HPLC-digitized fingerprint spectrum (HPLC-DFPS) of Rhizoma Pinelliae so that it will be applied in quality control of Rhizoma Pinelliae. METHODS: A gradient separated method was applied. Column: Agilent Zorbax ODS C_ 18 ; Mobile phase: acetonitrile-water; Detection-wavelength: 210 nm; Flow rate: 0.5 mL/min; Column temperature: 25℃. RESULTS: Using above optimum chromatograph condition, the HPLC-DFPS and technic parameters of Rhizoma Pinelliae were established. CONCLUSION: Comparison of a group of characteristic peaks suggests that the HPLC-DFPS technology is feasible in quality control of Rhizoma Pinelliae.

Study on Percolation Processes of Rhizoma Pinelliae(Processed with Ginger) / 中成药

Objective: To study the optimum extraction conditions of alkaloid of Rhizoma pinelliae processecd with ginger.Methods:The alkaloid content was used as a marker.The percolation process of Rhizoma Pinellia(processed with ginger) was studied by orthogonal design with 3 factors and 3 levels(L 9(3 4)) .Results:The alcohol concentration was remarkable factor in the test.Conclusion:Considering the demand of manufacture,the best extraction condition is A 3B 1C 1。

Progress of studies on preparation of Rhizoma Pinelliae / 中成药

To review and summarize the studies on preparation of Rhizoma Pinelliae in the recent years, including cleaning processing, preparing technology, and influenc e on chemical ingredients and pharmacological effects caused by prepara tion.

Determination of succinic acid in Rhizoma Pinelliae in various areas by TCL-scanning / 中成药

Objective: To compare Succinic Acid of Rhizoma Pinelliae in viarious areas in China.Method: Using the technology of TCL.Result:Succinic Acid in various areas in China are not very close to one another.The highest amount is in Shanxi Province(about 0.0254% )and the lowest amount is in Hunan Province(about 0.0038% ).Conclusion:There are obvious differences of Succinic Acid among various areas in China.Succinic Acid shoule be between 0.0038%～0.0254%

Study on Processing for Rhizoma Pinelliae (Processed with Licorice and Lime)by Orthogonal Design / 中成药

The procedure for Rhizoma Pinelliae(Processed with Licorice and Lime) was combinatively evaluated by L_9(3)~4 orthogonal design with three factors:decocting times for Radix Glycyrrhizae,concentrated volume of decoction and macerating time. The optimum condition is as follows: each 100g of Rhizoma Pinelliae,accompanied with 15g of Radix Glycyrrhizae (decocted with water for two times,then the two decoctions were combined,and concentrated to 150ml.)and 10g of calx,was macerated in concentrated decoction of Radix Glyeyrrhizae for six days