ABSTRACT
OBJECTIVE: This report presents a summary of the outcomes achieved at ART center FERTILAB in Buenos Aires, Argentina, with transport IVF-ICSI for 25 years (1990-2014). METHODS: This report included all patients submitted to oocyte retrieval for IVF-ICSI whose oocytes were transported from sites of aspiration located 0.5-58 kilometers away from the central laboratory. The numeric data herein reported were taken from annual reports submitted by our institution (Fertilab) and, for purposes of comparison, by all Argentinian centers to the Latin American Registry of Assisted Reproduction (RLA) within the same time period. RESULTS: From 1990 to 2014, 5091 aspirations followed by oocyte transport were performed in our center, resulting in 1258 pregnancies after fresh embryo transfers. The mean pregnancy/aspiration rate for the 25-year period was 24.71%. To validate the efficacy of our transport system, our results were compared to the outcomes of Argentinian centers reporting to RLA Argentina in the period ranging from 1990 to 2014. A total of 79,062 aspirations were performed, yielding 20,047 pregnancies and a pregnancy/aspiration rate of 25.36%. Delivery/aspirations rates were 15.34% for Fertilab patients and 14.79% for RLA Argentina centers. CONCLUSION: The results showed that the differences in clinical outcomes between our center and the bulk of Argentinian centers were not statistically significant, indicating that oocyte transport does not decrease the effectiveness of IVF-ICSI and might be advantageous under certain circumstances.
Subject(s)
Specimen Handling/adverse effects , Sperm Injections, Intracytoplasmic , Adult , Argentina , Embryo Transfer , Female , Humans , Oocyte Retrieval , Pregnancy , Pregnancy Rate , Specimen Handling/methodsABSTRACT
OBJECTIVE: To confirm the beneficial effect of endometrial coculture in patients with repeated failures with assisted reproductive techniques (ART). DESIGN: Patients with previous failures were offered a repetition of ART in conjunction with autologous endometrial coculture. SETTING: Private fertility center. PATIENT(S): Sixty-eight couples who had attempted 92 cycles of IVF or intracytoplasmic sperm injection without obtaining an evolutive pregnancy. INTERVENTION(S): Patients repeated one cycle of ART with concomitant endometrial coculture of their embryos. MAIN OUTCOME MEASURES(S): Comparative pregnancy and delivery rates in conventional ART cycles vs. cycles with autologous endometrial coculture. RESULT(S): In the previous 92 cycles (146 ETs, fresh plus frozen) only 8 pregnancies were initiated, and all ended in spontaneous abortion. Upon repeating 68 cycles (76 ETs) using coculture, 39 pregnancies were obtained, of which 19 resulted in live births, 10 are ongoing evolutive pregnancies, and 10 ended in spontaneous abortions. CONCLUSION(S): These results confirm the usefulness of autologous endometrial coculture for the treatment of patients with repeated implantation failure.
Subject(s)
Embryo Culture Techniques/methods , Embryo Implantation , Endometrium/cytology , Fertilization in Vitro , Infertility, Female/therapy , Pregnancy Outcome , Adult , Coculture Techniques , Female , Humans , Pregnancy , Sperm Injections, IntracytoplasmicABSTRACT
Two-cell murine embryos were cultured for 72 h in the presence or absence of granulocyte-macrophage colony stimulating factor (GM-CSF), frozen for 60 days and, after thawing, cultured for an additional 24 h in the presence or absence of GM-CSF. During the initial 72 h period, GM-CSF did not influence the percentage of embryos reaching the expanded blastocyst stage, but there was a significant increase (P < 0.05) in the number of cells in the embryos grown with GM-CSF. Survival after thawing was not affected by previous exposure to GM-CSF, but re-expansion of the blastocoele was diminished in that group. Exposure to GM-CSF during the post-thaw period greatly enhanced re-expansion of the blastocoele. The presence of human serum albumin in the culture media is thought to have masked the beneficial effect of GM-CSF upon embryos.
Subject(s)
Blastocyst/drug effects , Cryopreservation , Embryonic Development/drug effects , Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology , Animals , Blastocyst/cytology , Blastocyst/physiology , Cell Proliferation/drug effects , Culture Media , Female , Humans , Mice , Serum AlbuminABSTRACT
PROBLEM: To determine the ability of IgGs isolated from follicular fluids (hFFIgGs) to induce the acrosome reaction (AR) in human spermatozoa and to inhibit sperm-zona pellucida (ZP) interaction. METHOD OF STUDY: Incubation of capacitated spermatozoa with hFFIgGs (n = 40) and assessment of their effect on the AR or hemizona (HZ) assay in a condition that allows sperm-ZP interaction, avoiding acrosomal exocytosis. RESULTS: hFFIgGs from different women varied in their ability of inducing the AR. Those hFFIgGs with the highest AR-inducing capacity evoked the exocytotic response in most of the different sperm donors tested [high Induction Frequency (IF)]. Some of these antibodies were also able of inhibiting sperm binding to ZP [low HZ Index (HZI)]. A significant correlation was found between the IF and the HZI for each hFFIgG. CONCLUSIONS: Human follicular fluid contains antibodies capable of inducing the AR and inhibiting sperm-ZP binding, suggesting that they could be directed towards ZP receptors. hFFIgGs would constitute a tool for the identification of sperm entities involved in fertilization.
Subject(s)
Acrosome Reaction , Follicular Fluid/immunology , Immunoglobulin G/pharmacology , Spermatozoa/drug effects , Zona Pellucida/drug effects , Adult , Female , Follicular Fluid/metabolism , Humans , Male , Spermatozoa/immunology , Spermatozoa/metabolism , Zona Pellucida/immunology , Zona Pellucida/metabolismABSTRACT
La interacción inicial entre las gametas es mediada por proteínas de superficie de la cabeza del espermatozoide y de la matriz extracelular del ovocito, la zona pellucida (ZP). El presente trabajo tuvo como objetivo la identificación de antígenos de superficie de espermatozoides humanos potencialmente involucrados en el reconocimiento de la ZP. Para ello se obtuvo un extracto de proteínas periféricas de espermatozoides humanos por tratamiento de las células con solución de alta fuerza iónica (HSE - High Salt Extract) (Buffer Pipes 100mM, pH 7,4; NaCl1M, sacarosa 0,25 M). Se desarrollaron anticuerpos policlonales (anti-HSE) que reconocieron numerosas proteínas en HSE (9-200 KDa). Las proteínas fueron separadas por electroforesis en geles de poliacrilamida, transferidas a membranas de nitrocelulosa y utilizadas para adsorber inmunoglobulinas de anti-HSE. Con éste método se obtuvieron anticuerpos contra dos polipéptidos mayoritarios de 49 (p49) y 66 (p66) kDa. Ambos anticuerpos (anti-p49 y anti-p66) reconocieron epitopes localizados en la cabeza y el flagelo de espermatozoides eyaculados y capacitados...(AU)
Subject(s)
Humans , Male , Female , Sperm-Ovum Interactions/immunology , Antigens, Surface , Sperm-Ovum Interactions/physiology , Zona Pellucida , Spermatozoa/immunology , Immunodominant Epitopes , Antibodies , Sperm Head/immunologyABSTRACT
La interacción inicial entre las gametas es mediada por proteínas de superficie de la cabeza del espermatozoide y de la matriz extracelular del ovocito, la zona pellucida (ZP). El presente trabajo tuvo como objetivo la identificación de antígenos de superficie de espermatozoides humanos potencialmente involucrados en el reconocimiento de la ZP. Para ello se obtuvo un extracto de proteínas periféricas de espermatozoides humanos por tratamiento de las células con solución de alta fuerza iónica (HSE - High Salt Extract) (Buffer Pipes 100mM, pH 7,4; NaCl1M, sacarosa 0,25 M). Se desarrollaron anticuerpos policlonales (anti-HSE) que reconocieron numerosas proteínas en HSE (9-200 KDa). Las proteínas fueron separadas por electroforesis en geles de poliacrilamida, transferidas a membranas de nitrocelulosa y utilizadas para adsorber inmunoglobulinas de anti-HSE. Con éste método se obtuvieron anticuerpos contra dos polipéptidos mayoritarios de 49 (p49) y 66 (p66) kDa. Ambos anticuerpos (anti-p49 y anti-p66) reconocieron epitopes localizados en la cabeza y el flagelo de espermatozoides eyaculados y capacitados...
Subject(s)
Humans , Male , Female , Antigens, Surface , Sperm-Ovum Interactions/immunology , Antibodies , Immunodominant Epitopes , Sperm-Ovum Interactions/physiology , Sperm Head , Spermatozoa , Zona PellucidaABSTRACT
Determinar la posibilidad de utilizar un laboratorio central defertilización in vitro al cual se trasportan los ovocitos desde el sitio de su recuperación. Determinar si ese trasporte altera la capacidad de los mismos para ser fertilizados y dar origen a embriones viables.
Subject(s)
Comparative Study , Humans , Male , Female , Pregnancy , Adult , Reproductive Techniques/statistics & numerical data , Fertilization in Vitro/methods , Oocytes , Fertilization in Vitro/statistics & numerical data , Fertilization in Vitro/standards , Reproductive Techniques/instrumentation , Reproductive Techniques/standards , Embryonic Development , Infertility/epidemiology , Infertility/therapy , Oocytes/growth & development , Oocytes/drug effectsABSTRACT
Determinar la posibilidad de utilizar un laboratorio central defertilización in vitro al cual se trasportan los ovocitos desde el sitio de su recuperación. Determinar si ese trasporte altera la capacidad de los mismos para ser fertilizados y dar origen a embriones viables.