ABSTRACT
The activity of receptor-operated Ca2+ channels (ROCCs) was studied in rat portal vein in L-thyroxine-induced experimental hyperthyroidism. The following parameters were evaluated: 1. NE-stimulated 45Ca influx. 2. CaCl2-induced contractile responses in Ca2+ free NE-stimulated tissues to calculate EC50 value of CaCl2. The NE (10(-6)mol) stimulated 45Ca influx and the mean EC50 value of CaCl2 did not differ significantly in portal veins isolated from hyperthyroid rats as compared to those of euthyroid control rats. The study revealed no significant change in the functional status of ROCCs in experimental hyperthyroidism.
Subject(s)
Animals , Calcium Channels/metabolism , Calcium Signaling , Hyperthyroidism/chemically induced , Male , Muscle, Smooth, Vascular/metabolism , Portal Vein/metabolism , Rats , Rats, Sprague-Dawley , Receptors, Adrenergic, alpha/metabolism , Thyroxine/toxicityABSTRACT
Effect of pinacidil, a K+ channel opener, was studied on contractility of cyclophosphamide-treated rat vas deferens. The mean IC50 value of pinacidil against 1 mmol barium chloride induced rhythmic contractions and 40 mmol potassium chloride induced tonic contractions was significantly (P < 0.01 and P < 0.001, respectively) increased in the cyclophosphamide treated group as compared to the control. The mean EC50 value of norepinephrine (NE) in the presence of pinacidil (10(-6) mol) was significantly (P < 0.001) increased in the cyclophosphamide treated group. These findings indicate that the responsiveness of rat vas deferens smooth muscle to pinacidil is reduced following cyclophosphamide treatment.
Subject(s)
Animals , Antineoplastic Agents, Alkylating/toxicity , Calcium/metabolism , Cyclophosphamide/toxicity , Male , Norepinephrine/pharmacology , Pinacidil/pharmacology , Potassium Channels/drug effects , Rats , Vas Deferens/drug effectsABSTRACT
Possible central modulation of acute peripheral inflammation by putative amino acid neurotransmitters was investigated in rats by adopting formalin induced pedal inflammation as an experimental model. Out of five amino acids (GABA, glycine, DL-alanine, L-glutamic acid and L-aspartic acid) tested, intracerebroventricular (icv) administration of GABA and L-aspartic acid produced significant alteration in acute inflammation. GABA showed a significant attenuation of paw oedema and nociception whereas, L-aspartic acid produced significant increase in oedema volume along with marked hyperalgesia. In conclusion, the study confirms that CNS is capable of modulating peripheral inflammation.
Subject(s)
Acute Disease , Amino Acids/pharmacology , Animals , Aspartic Acid/pharmacology , Brain/physiology , Formaldehyde , Inflammation/physiopathology , Male , Neurotransmitter Agents/pharmacology , Pain Threshold/drug effects , Rats , Rats, Sprague-Dawley , gamma-Aminobutyric Acid/pharmacologyABSTRACT
The possibility of central noradrenergic and dopaminergic modulation of Brewer's yeast-induced peripheral inflammation was investigated in rats. Centrally administered noradrenaline (NA), amphetamine, which liberates NA and dopamine in the central nervous system and L-dopa, the precursor of dopamine significantly suppressed paw oedema. Conversely, the beta-adrenoceptor blocker, propranolol, catecholaminergic neuron degenerator, 6-hydroxydopamine (6-OHDA), dopaminergic antagonist, haloperidol and dopamine synthesis inhibitor, alpha-methyl para tyrosine (AMPT) augmented paw oedema. In addition, 6-OHDA and haloperidol produced significant reduction in pain threshold. The results of this study indicate that central NA and dopamine exert inhibitory effects on Brewer's yeast-induced peripheral inflammation.
Subject(s)
Amphetamine/administration & dosage , Animals , Dopamine/physiology , Inflammation/physiopathology , Male , Norepinephrine/administration & dosage , Pain/physiopathology , Rats , Rats, Sprague-Dawley , Saccharomyces cerevisiae , Sympathomimetics/administration & dosageABSTRACT
Possible modulation of the Brewer's yeast-induced peripheral inflammation by two central neuropeptides, bradykinin and substance P (SP), was investigated in rats. Centrally administered bradykinin significantly increased pedal oedema and pain threshold whereas, SP produced significant augmentation of oedema volume and nociception. The results of the present study indicate that central bradykinin exerts pro-inflammatory and analgesic effects whereas, central SP exerts pro-inflammatory and pro-nociceptive effects on Brewer's yeast-induced peripheral inflammation.
Subject(s)
Analgesics/pharmacology , Animals , Bradykinin/pharmacology , Edema/drug therapy , Inflammation/drug therapy , Male , Pain/drug therapy , Rats , Rats, Sprague-Dawley , Saccharomyces cerevisiae , Substance P/pharmacologyABSTRACT
Possible central serotonergic and histaminergic modulation of acute peripheral inflammation was investigated in rats, adopting the formaldehyde-induced acute pedal inflammation as an experimental model. Intracerebroventricular (icv) administration of central inhibitory neurotransmitter, serotonin and its precursor, 5-hydroxytryptophan (5-HTP) attenuated the oedema volume and exudate protein content alongwith augmentation in pain threshold. On the contrary, cyproheptadine, a 5-HT-receptor antagonist and selective serotonin synthesis inhibitor, parachlorophenylalanine (PCPA) produced oedema augmenting and pro-nociceptive effects besides elevating the protein content of the exudate. Centrally administered histamine attenuated pedal oedema, nociception as well as protein concentration in oedema fluid. Cimetidine, an H2 histaminergic receptor blocker did not produce any significant effect on inflammation.
Subject(s)
Animals , Foot/pathology , Formaldehyde , Histamine/administration & dosage , Histamine Agonists/pharmacology , Histamine Antagonists/pharmacology , Inflammation/chemically induced , Injections, Intraventricular , Male , Nociceptors/physiology , Pain/chemically induced , Pain Threshold/drug effects , Rats , Rats, Sprague-Dawley , Serotonin/administration & dosage , Serotonin Receptor Agonists/pharmacology , Serotonin Antagonists/pharmacology , Time FactorsABSTRACT
Possible central noradrenergic and cholinergic modulation of acute peripheral inflammation was investigated in rats, adopting the formaldehyde-induced pedal inflammation as the experimental model. Intracerebroventricularly (icv) administered noradrenaline (NA), alpha-adrenoceptor agonist, L-phenylephrine, alpha-2 adrenoceptor agonist, clonidine and non-selective beta-adrenoceptor blocker, propranolol, suppressed formaldehyde-induced inflammation producing a decrease in oedema volume and increase in pain threshold. Conversely, both noradrenergic neuron degenerator, 6-hydroxydopamine (6-OHDA) and non-selective alpha-adrenoceptor antagonist, phenoxybenzamine produced an increase in paw oedema along with an augmentation of pain. Significant oedema augmenting effects were also produced by central excitatory neurotransmitter, acetylcholine (ACh) on icv administration. ACh also produced pro-nociceptive action. An ACh antagonist, scopolamine and ACh synthesis inhibitor, hemicholinium-3 (HC) reduced pedal oedema and produced analgesia. The results of this study indicate that central NA exerts an inhibitory effect on peripheral oedema and pain whereas, ACh has an augmenting effect on formaldehyde-induced peripheral inflammation.