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1.
Braz. j. oral sci ; 21: e226288, jan.-dez. 2022. ilus
Article in English | LILACS, BBO | ID: biblio-1366204

ABSTRACT

Aim: To describe the pattern of toothache experience in a cohort of children aged 2-5 over 21 years and to find the relationship between previous toothache experience and later reports of toothache to get finding on the most critical period of toothache as a problem in life. Methods: This is a secondary data analysis from Indonesia Family Life Survey (IFLS). A total of 1,927 children from IFLS-1 with complete data were included as baseline participants. They were followed up four times within 21 years (age 6-9y, age 9-12y, age 16-19y, age 23- 26y). Toothache was based on the question of self-reported toothache experience during the last four weeks. After 21 years, a total of 1,098 individuals could be traced and completed every cohort of the survey. Toothache experiences were reported for frequencies in every cohort and accumulative experiences over 21 years. Logistic regression tests were performed to analyze the association of previous toothache experience and later toothache experience. Results: Almost 40% of the respondents reported toothache at least once in their life. The age of 6-9 years is the period when a high percentage of children had teeth-related pain. The experience of toothache at this period was significantly related to every period of age in life. Conclusions: The period of early mixed dentition is important. Oral health status in this period is associated with future oral health. A comprehensive dental health prevention program targeting this population is essential to increase the quality of life


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Young Adult , Toothache/epidemiology , Oral Health , Socioeconomic Factors , Dental Health Surveys , Prevalence , Longitudinal Studies , Indonesia/epidemiology
2.
J. pediatr. (Rio J.) ; 97(2): 197-203, Mar.-Apr. 2021. tab, graf
Article in English | LILACS | ID: biblio-1287031

ABSTRACT

Abstract Objective: Irritable bowel syndrome is a frequent functional gastrointestinal disorder. The aims of this study were to investigate its epidemiology, focusing on the role of intestinal mucosal integrity and to evaluate the impact on the quality of life. Methods: A community-based survey applying a comparative cross sectional approach was conducted in six high schools in Palembang. Subjects were recruited using multistage random sampling divided in two groups. Rome III criteria were used to establish a diagnosis of IBS in combination with a questionnaire to determine risk factors. Determination of fecal alpha-1-antitrypsin and calprotectin levels was performed to determine impaired intestinal mucosal integrity. A questionnaire was used to evaluate how quality of life was affected by irritable bowel syndrome. Results: The survey was performed in 454 14−18 years old adolescents, of whom 30.2% fulfilled the Rome III criteria for IBS, with the following subtypes: 36.5% diarrhea, 18.9% constipation, 21.9% mixed, and 22.6% unclassified. Major risk factors were female gender, bullying, age 14-16 years, history of constipation and diarrhea, eating nuts, and drinking coffee, tea, and soft drinks. There was a significant association with intestinal inflammation (p = 0.013). A significantly impaired quality of life was found (p = 0.001). Conclusions: The prevalence of irritable bowel syndrome in adolescents was high, with bullying, female gender, age 14-16 years, constipation and diarrhea, and dietary consumption of soft drinks, coffee, and tea as risk factors. A significant association with intestinal inflammation was found.


Subject(s)
Humans , Male , Female , Adolescent , Irritable Bowel Syndrome/epidemiology , Quality of Life , Cross-Sectional Studies , Surveys and Questionnaires , Constipation/etiology , Constipation/epidemiology , Diarrhea/etiology , Diarrhea/epidemiology , Indonesia/epidemiology
3.
Article in English | WPRIM | ID: wpr-922378

ABSTRACT

OBJECTIVES@#To study the association between milk consumption and lactose malabsorption in Indonesian children aged 3-12 years.@*METHODS@#This cross sectional study was conducted in randomly selected presumed healthy children with good nutritional status aged 3-12 years in Central Jakarta, Indonesia (@*RESULTS@#The prevalence of lactose malabsorption in children aged 3-5 years and children aged 6-12 years was 20.8% (15/72) and 35.3% (36/102), respectively. There was no association between milk or milk product consumption and lactose malabsorption (@*CONCLUSIONS@#There is no association between milk consumption and lactose malabsorption in Indonesian children aged 3-12 years, suggesting that genetic predisposition may be more important than adaptive mechanisms to lactose consumption.


Subject(s)
Animals , Breath Tests , Child , Cross-Sectional Studies , Humans , Indonesia/epidemiology , Lactose Intolerance/epidemiology , Milk
4.
Article in English | LILACS, BBO | ID: biblio-1143394

ABSTRACT

ABSTRACT Objective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its growth measured by spectrophotometer. The saliva viscosity was analyzed by viscometer, and pH of saliva was measured by pH meter. Results: Based on the optical density values, growth of S. mutans in saliva ranged <300 CFU/mL (0.1 nm) at concentrations of 25%, 12.5% and 6.25% for 24 hours. Whereas at the 48 h and 72 h period of incubation shown an increase in growth of S. mutans ranged 300-600 CFU/mL (0.2-0.36 nm). The inhibitory biofilm formation of S. mutans in saliva was significantly higher at concentrations of 12.5% and 6.25% at 24 h incubation times on a moderate scale, whereas the histatin-5 was effective to inhibit S. mutans biofilm on the 50 and 25 ppm. The saliva possessed a higher inhibitory of biofilm S. mutans than histatin-5 and good level viscosity (0.91-0.92 cP). Conclusion: The saliva was able to control the growth of S. mutans, and histatin-5 can inhibit the biofilm formation S. mutans. Furthermore, the saliva was also able to respond to the pH change with good viscosity of saliva.


Subject(s)
Humans , Male , Female , Child , Saliva/microbiology , Biofilms , Viridans Streptococci , Histatins , Hydrogen-Ion Concentration , Spectrophotometry/instrumentation , Streptococcus mutans , Viscosity , Analysis of Variance , Statistics, Nonparametric , Indonesia/epidemiology
5.
Article in English | LILACS, BBO | ID: biblio-1346682

ABSTRACT

Abstract Objective: To determine differences in how orthodontic patients perceive the aesthetics relating to the use of Essix and Hawley retainers. Material and Methods: Photographs of a female patient using an Essix retainer and a Hawley retainer, respectively, were assessed by 70 orthodontic patients aged between 21 and 55 years. All participants were provided with the same images of the two retainer types being worn and were asked to grade the aesthetics of each retainer using a visual analog scale. The significance of differences in orthodontic patient perceptions of aesthetics in the use of Essix and Hawley Retainers in the young adult and adult age groups were tested using the Mann-Whitney test with assumed significance p<0.01. Results: There were differences in participants' perceptions between the two retainers that were statistically significant, with responses varying between young adults aged 21 to 35 years and adults aged 36 to 55 years. According to the VAS, the two retainers' average scores in the total population are 82 for Essix Retainer and 60 for Hawley Retainer in both groups. Conclusion: The Essix retainer as a retention appliance is considered more aesthetically pleasing than the Hawley retainer among both young and middle-aged adults.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Orthodontic Appliances , Orthodontic Retainers , Photography, Dental/instrumentation , Visual Analog Scale , Epidemiology, Descriptive , Surveys and Questionnaires , Statistics, Nonparametric , Data Accuracy , Indonesia/epidemiology
6.
Article in English | LILACS, BBO | ID: biblio-1143389

ABSTRACT

ABSTRACT Objective: To determine the relationship of transmission of COVID-19 virus knowledge with dental care during a pandemic. Material and Methods: Online questionnaire using Google forms distributed through social media (WhatsApp groups, Facebook, line groups) using a combination of convenience and snowball sampling. The level of knowledge about coronavirus transmission was measured using a questionnaire consisting of 5 statement items. This questionnaire uses the Guttman scale with 3 response and anxiety categories for dental care as measured using a modified questionnaire from the Dental Fear Survey. This questionnaire consisted of 9 question items using a Likert scale. Results: 285 respondents came from WhatsApp media users (89.9%) with an average age of 29.91 years. Respondents who experienced "dental health problems" during the pandemic were 44.7%. The knowledge of respondents about the transmission of the COVID-19 virus in dental care was 79.9% and the knowledge of the fear of caring during the pandemic period was 31.85% afraid. Statistical analysis showed a significant relationship between knowledge about transmission of the COVID-19 virus and fear of dental care (rs=0.388, p<0.001). Conclusion: The results obtained have a significant relationship between knowledge about transmission and fear for dental care during the COVID-19 pandemic in South Sulawesi Province.


Subject(s)
Humans , Male , Female , Dental Care , Dental Anxiety , SARS Virus , COVID-19/pathology , Indonesia/epidemiology , Surveys and Questionnaires , Statistics, Nonparametric , Fear , Pandemics , Social Media
7.
Article in English | LILACS, BBO | ID: biblio-1287497

ABSTRACT

ABSTRACT Objective: To analyze periodontal comparison between Systemic Lupus Erythematosus (SLE) subject and healthy control. Material and Methods: This descriptive cross-sectional study included 122 subjects, 61 SLE patients and 61 healthy subjects who visited the Rheumatology Department, Dr. Saiful Anwar General Hospital, Malang, during 2017-2018. Clinical examination of SLE was using Mexican SLE Disease Activity Index and oral cavity conditions were assessed using the periodontal index, gingival index, calculus index, bleeding on probing, clinical attachment loss and mobility teeth. Results: The age of SLE patients ranged from 18-55 years old with the mean age of 29.50 ± 9.57 years old. Periodontitis was higher in SLE patients (88.5%) than healthy subjects (22.95%). In addition, periodontitis occurrence in SLE (2.66 ± 1.02) was significantly different (p<0.001) compared to healthy subjects (0.51 ± 0.81). Conclusion: This study found higher rates of periodontitis, gingivitis, bleeding on probing, clinical attachment loss, and mobility tooth among SLE patients compared to healthy subjects. Periodontitis was also found to be higher along with more severe SLE group.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Periodontal Diseases/pathology , Tooth Mobility , Periodontal Index , Connective Tissue Diseases , Lupus Erythematosus, Systemic/pathology , Periodontitis , Clinical Diagnosis , Dental Plaque Index , Oral Health , Epidemiology, Descriptive , Cross-Sectional Studies/methods , Data Interpretation, Statistical , Statistics, Nonparametric , Gingivitis , Indonesia/epidemiology
8.
Article in English | LILACS, BBO | ID: biblio-1287491

ABSTRACT

Abstract Objective: To determine the level of biofilm formation of S. mutans after being exposed to 5% sucrose, 8% lactose, or 1% xylitol. Material and Methods: This research was a laboratory-based experimental study with post-test only control group design. S. mutans was grown in test tubes containing tryptose soy broth (TSB) medium supplemented with 1% glucose. They were incubated at 37° C for 24 hours to grow the biofilms. The culture was then exposed to 5% sucrose, 8% lactose or 1% xylitol, incubated for 24 hours at 37° C, and examined using ELISA at a wavelength of 625 nm. The statistical analysis was performed using a one-way analysis of variance followed by the least significant difference test (a=0.05). Results: There were some differences in the biofilm formation of S. mutans after exposure to 5% sucrose, 8% lactose, or 1% xylitol (p<0.05). An LSD test indicated significant differences among the biofilm formations after exposure to 5% sucrose and 8% lactose and between 5% sucrose and 1% xylitol. In comparison, there were no significant differences (p>0.05) between 8% lactose and 1% xylitol. Conclusion: Sucrose, lactose and xylitol can form biofilms and the formation of lactose biofilms is the same as xylitol.


Subject(s)
Streptococcus mutans/immunology , Sucrose/adverse effects , Xylitol , Disaccharides , Indonesia/epidemiology , Enzyme-Linked Immunosorbent Assay , Epidemiology, Experimental , Analysis of Variance , Biofilms , Dental Plaque
9.
Article in English | LILACS, BBO | ID: biblio-1250459

ABSTRACT

Abstract Objective: To measure the level of immunoglobulin G (IgG) in the gingival crevicular fluid (GCF). Material and Methods: A total of 158 patients aged >45 years were examined for periodontitis and interviewed regarding their menopausal status. The non-menopause group entailed female patients with periodontitis without menopause (n=23). The menopause group included females who stopped menstruating since >1 year, had a pocket depth of 4-5 mm, and did not have other systemic conditions (n=40). Samples were selected based on periodontal and menopausal status. In total, 63 samples of GCF were collected from the participants and tested using an enzyme-linked immunosorbent assay kit for IgG. Results: The median level of IgG in the menopause group was 39.50 (g/mL, whereas that of the non-menopause group was 41.08 (g/mL. There was a positive correlation between the plaque index and IgG level in both groups. In contrast, there was a negative correlation between age and IgG level. However, there was no correlation between plaque index and age regarding the IgG level in both groups (p>0.05). Conclusion: The IgG levels in the menopause group were lower than those in the non-menopause group. As such, menopausal females should take great care of their overall health, including the periodontium.


Subject(s)
Humans , Female , Adult , Middle Aged , Periodontal Diseases/pathology , Periodontitis/pathology , Periodontium , Immunoglobulin G , Menopause , Cross-Sectional Studies/methods , Gingival Crevicular Fluid , Statistics, Nonparametric , Indonesia/epidemiology
10.
Article in English | LILACS, BBO | ID: biblio-1180860

ABSTRACT

ABSTRACT Objective: To describe the gonial angle characteristics in class III skeletal malocclusion in Javanese ethnic. Material and Methods: Pretreatment lateral cephalometric radiographs of 43 Javanese ethnic patients were measured: upper (Go1) and lower gonial angle (Go2), anterior (AFH) and posterior face height ratio (PFH), maxilla-mandibular length difference, mandibular plane angle (FMA), Y axis, ramus position, ANB angle, posterior cranial base/ramus height and mandibular body length/anterior cranial base. The relation between Go1, Go2 and other variables were analysed using correlation and regression analysis. Results: The total gonial angle is within normal range, but Go1 is below normal and Go2 is above normal. There is no difference between male and female gonial angle measurements (p=0.939 and p=0.861, respectively). Ramus position is positively correlated to Go1 (p=0.003), while AFH (p=0.000), maxilla-mandibular length difference (p=0.000), FMA (p=0.000), Y axis (p=0.000), and posterior cranial base/ramus height (p=0.018) are positively correlated to Go2. PFH is negatively correlated to Go2 (p=0.018). Conclusion: The upper gonial angle is influenced by the position of mandibular ramus, while the lower gonial angle is affected by the posterior and anterior lower facial height and mandibular size and rotation. Javanese with class III malocclusion tends to have hypodivergent facial type, with more posteriorly located mandibular ramus and excess mandibular length.


Subject(s)
Humans , Male , Female , Adult , Orthodontics , Cephalometry/methods , Indonesia/epidemiology , Malocclusion, Angle Class III/therapy , Mandible/anatomy & histology , Regression Analysis , Data Interpretation, Statistical , Maxilla/diagnostic imaging
11.
Article in English | LILACS, BBO | ID: biblio-1180859

ABSTRACT

ABSTRACT Objective: To evaluate the improvement in masticatory performances two weeks after posterior implant restoration. Material and Methods: Nine patients with missing first and second molars were included in the study. Masticatory performances were evaluated using subjective and objective methods utilizing color-changeable chewing gum and the visual analog scale (VAS) assessment. The subjects were asked to chew the color-changeable chewing gums before and two weeks after the posterior implant restoration. Their expectations regarding aesthetics and function were verified on the VAS before implant placement. Additionally, the VAS was used for the posttreatment completion rating two weeks after the implant restoration. Results: Significant differences in masticatory performance were noted at baseline (before implant posterior restoration) and two weeks after implant restoration (p<0.05). The posttreatment aesthetic and functional expectations ratings significantly exceeded the expectations (p<0.05). Conclusion: Masticatory performances were improved two weeks after implant restoration. In addition, the significant posttreatment ratings of the patients exceeded their initial expectations. In particular, patients with poor masticatory functions demonstrated significant improvements and satisfaction following implant restoration compared to those with good mastication. These findings indicate that a posterior implant restoration can increase the masticatory performance.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Chewing Gum/microbiology , Patient Satisfaction , Dental Implantation/instrumentation , Molar/anatomy & histology , Statistics, Nonparametric , Visual Analog Scale , Indonesia/epidemiology , Mastication
12.
Article in English | LILACS, BBO | ID: biblio-1155006

ABSTRACT

ABSTRACT Objective: To evaluate the regeneration of mandibular cartilage defect after implantation of human umbilical cord mesenchymal stem cells (hUCMSC) over platelet rich fibrin (PRF) as scaffold. Material and Methods: 20 male Wistar rats were randomly divided into four experimental groups consisting of: a control group featuring untreated mandibular defects (C), experimental groups whose mandibular defects were implanted with hUCMSC (E1), mandibular defects implanted with PRF (E2), mandibular defects implanted with hUCMSC and PRF scaffold (E3). The subjects were sacrificed after six weeks of observation for immunohistochemical examination in order to evaluate the expression of Ki67, Sox9, FGF 18, type 2 collagen, and aggrecan, in addition to histology examination to evaluate chondrocyte number and cartilage thickness. Data was analyzed with univariate analysis (ANOVA). Results: The implantation of hUCMSC and PRF scaffold proved capable of regenerating mandibular cartilage defect through the expression of FGF 18, Sox9, Ki67, chondrosis counts, type 2 collagen, aggrecan, and cartilage thickness. The regeneration were significantly higher in group E3. Conclusion: Human umbilical cord mesenchymal stem cells in platelet rich fibrin scaffold proved capable of regenerating mandibular cartilage defect.


Subject(s)
Animals , Rats , Cartilage , Cord Blood Stem Cell Transplantation , Regenerative Medicine , Mesenchymal Stem Cells/microbiology , Platelet-Rich Fibrin/microbiology , Immunohistochemistry , Epidemiology, Experimental , Analysis of Variance , Rats, Wistar , Indonesia/epidemiology
13.
Article in English | LILACS, BBO | ID: biblio-1135569

ABSTRACT

Abstract Objective: To evaluate the knowledge and behaviour of mothers in maintaining their oral health during pregnancy. Material and Methods: This study included 167 women in five community primary health centres in Central Jakarta whose last child ranged between 0-59 months. The participants were asked to fill the questionnaire "Knowledge and Behaviour Towards Oral Health during Pregnancy" (KBOHP). This questionnaire assessed the knowledge and behaviour in maintaining oral health during pregnancy. Results: Almost 80% of participants reported that they had government dental health coverage. Only about 16% of participants reported that they visit dentist before pregnancy and only about 32% of the participants reported to have oral health problems during pregnancy. Although they have oral health problems, only 18% of them visited the dentists during pregnancy. Only 33% of the participants knew that the periodontal problems can affect pregnancy outcome, and 54.5% of participants had never heard of a possible connection between oral health and pregnancy. About 74% of participants reported that they never had specific oral health care instruction during pregnancy. Participants who received oral health instruction from their health providers also tend to have dental visit during pregnancy. Conclusion: Knowledge and behaviour towards oral health during pregnancy in this population was still low. A program that is developed to improve knowledge and behaviour in maintaining oral health during pregnancy is needed.


Subject(s)
Humans , Female , Adult , Pregnancy , Attitude of Health Personnel , Health Knowledge, Attitudes, Practice , Oral Health , Mothers , Oral Hygiene/education , Chi-Square Distribution , Cross-Sectional Studies/methods , Surveys and Questionnaires , Indonesia/epidemiology
14.
Article in English | LILACS, BBO | ID: biblio-1135549

ABSTRACT

Abstract Objective: To examine the cytotoxicity of calcium hydroxide on human umbilical cord mesenchymal stem cells (HUCMSC) to understand the characteristics for use in regenerative dentistry procedures especially regenerative endodontics. Material and Methods: HUCMSC was isolated, cultured, and confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSC was cultured and divided into two groups, the control group (cultured in minimum essential medium (MEM) alpha) and calcium hydroxide group (cultured in MEM alpha and calcium hydroxide). Methyl-thiazole-tetrazolium (MTT) assay was done on different concentrations of calcium hydroxide (0.39 to 25 µg/mL) and the cells were observed and counted. One-way ANOVA test was used with a significance level set at 5%. Results: Flow cytometric analysis confirmed positive of CD73, CD90, CD105, negative of CD45 and CD34. A significant difference was found between the concentration of 6.25 and 3.125 µg/mL (p=0.004). There was no significant difference among 6.25, 12.5 and 25 µg/mL concentrations. There was also no significant difference among 0.39, 0.78, 1.56, and 3.125 µg/mL concentrations. Conclusion: Even though calcium hydroxide is a medicament of choice in clinical endodontics, it decreases the viability of HUCMSC. The lower the concentration of calcium hydroxide, the higher the viability of HUCMSC.


Subject(s)
Humans , Calcium Hydroxide/therapeutic use , Cell Survival , Stem Cell Research , Mesenchymal Stem Cells , Regenerative Endodontics , Umbilical Cord , Analysis of Variance , Indonesia/epidemiology
15.
Article in English | LILACS, BBO | ID: biblio-1135546

ABSTRACT

Abstract Objective: To determine the prevalence of cross-sectional variations in the apical thirds of the root canals in maxillary and mandibular teeth. Material and Methods: Eighty tooth samples (maxillary second premolar, maxillary first molar, and mandibular first molar) were scanned using micro-computed tomography. The apical third area of each root canal was sectioned and the maximum and minimum diameters were calculated from the mesiodistal and buccolingual diameters. The shapes were categorized as a round, oval, long oval, and flat based on the ratios obtained. Results: The most common shape of the apical third of the root canals in the maxillary second premolars was oval (66.7%), followed by long oval (24.6%), flat (7%), and round (1.7%). The corresponding values in the mesiobuccal root of the maxillary first molars were oval (68.2%), long oval (22.7%), flat (9.1%), 94.1% of the distobuccal roots were oval, while the remaining were long oval (5.9%). All the palatal root canals were oval. In the mesiobuccal root of the mandibular first molars, 47.4% were long oval in shape, followed by 36.8% oval and 15.8% flat canals. All the mesiolingual root canals were oval, whereas, in the distal root, 68.4% were oval, 21.1% long oval, and 10.5% were flat. Conclusion: The oval shape was most commonly observed in the majority of the root canals. Knowledge about the apical anatomy of the root can help the operator improve the root canal treatment's success.


Subject(s)
Humans , Bicuspid , Anatomy, Cross-Sectional/instrumentation , Dental Pulp Cavity , Endodontics , Molar , Tomography, X-Ray Computed/instrumentation , Cross-Sectional Studies/methods , Data Interpretation, Statistical , Indonesia/epidemiology
16.
Article in English | LILACS, BBO | ID: biblio-1135538

ABSTRACT

Abstract Objective: To determine whether psychosocial status is associated with orthodontic treatment needs in high school students using the Psychosocial Impact of the Dental Aesthetic Questionnaire (PIDAQ) and the Index of Orthodontic Need (IOTN). Material and Methods: This cross-sectional study comprised 95 adolescents, both genders, aged 15-17 years old. PIDAQ was used to assess psychosocial status. The IOTN Aesthetic Component (IOTN-AC) and the IOTN Dental Health Component (DHC) was used to assess treatment needs. Examination of the oral cavity was carried out with the help of the IOTN ruler by observing the MOCDO components: missing teeth, overjet, crossbite, displacement, and overbite. The Chi-square test was used to determine the association of psychosocial status and orthodontic treatment need. The reliability test was carried out by using Cronbach's Alpha test (0.978). Results: There was an association between psychosocial status and orthodontic treatment need based on the IOTN-AC (p=0.001) and between psychosocial status and orthodontic treatment need based on the IOTN-DHC (p=0.140). Conclusion: There is an association between psychosocial status based on the PIDAQ and subjective orthodontic treatment need based on the IOTN-AC, but there is no association between psychosocial status based on the PIDAQ and objective orthodontic treatment need based on the IOTN-DHC in students of SMAN 27 Jakarta.


Subject(s)
Humans , Male , Female , Adolescent , Orthodontics , Dental Health Surveys/methods , Overbite , Index of Orthodontic Treatment Need/methods , Indonesia/epidemiology , Chi-Square Distribution , Cross-Sectional Studies/methods , Surveys and Questionnaires , Psychosocial Impact
17.
Article in English | LILACS, BBO | ID: biblio-1135530

ABSTRACT

Abstract Objective: To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2-3 years. Material and Methods: A cross-sectional survey was conducted and samples from 87 children aged 2-3 years, who lived in selected villages in the Bandung City area, Indonesia, were collected. Examination for dental caries was performed using standard checks for decay, missing, and filled surfaces (dfms), and saliva samples were taken. Microbiological examination was performed using RT-PCR with primers consisting of one primary set for Veillonella spp. and one universal primary set for 16S rDNA. We performed statistical testing using the Mann Whitney rank-sum test. Results: A total of 87 children were sampled, and an ECC prevalence of 71.3% was found, with a mean dmfs of 7.1 (± 9.1). The proportion of Veillonella spp. in caries-free children was 2.13 ± 2.30, while in children with ECC, it was 3.29 ± 6.83. Conclusion: The proportion of Veillonella spp. in children with ECC was higher than in caries-free children; therefore, Veillonella spp. may be a risk factor for ECC.


Subject(s)
Humans , Male , Female , Child, Preschool , Veillonella , Polymerase Chain Reaction/methods , Risk Factors , Dental Caries/prevention & control , Gram-Negative Bacteria/immunology , Epidemiologic Studies , Prevalence , Cross-Sectional Studies , Statistics, Nonparametric , Real-Time Polymerase Chain Reaction/methods , Indonesia/epidemiology
18.
Article in English | LILACS, BBO | ID: biblio-1135526

ABSTRACT

Abstract Objective: To determine the diversity of microbiota in the sub-gingival fluids from periodontitis and rheumatoid arthritis (RA) patients and to know the relationship of microbiota abnormalities between periodontitis and RA. Material and Methods: Samples were collected from sub-gingival fluids of nine participants by swabbing technique. The participants were divided into 3 groups, namely the control (not periodontitis and RA), periodontitis, and RA. The total number of bacteria was calculated by using direct culture in serial dilution by applying the total plate count method. Isolation of microbiota was conducted by pour plates and incubated in an anaerobic jar. The bacterial diversity was performed by Simpson index and DNA Isolation. Results: There were differences in the concentration of sub-gingival fluids bacteria in the control group with the periodontitis and RA group. The microbiota composition profiles were different for each group. The bacterial isolate 4, 5 and 22 were found in all groups, while isolate 14 was assumed to be related to the metabolic pathway in microbiota abnormalities associated with disease progression. Conclusion: The Simpson index has a value >0.61 with high bacterial dominance; however, the diversity of microbiota is at a low level of diversity. We assumed that isolate 14 was associated with development of RA disease.


Subject(s)
Humans , Periodontal Diseases/pathology , Periodontitis , Arthritis, Rheumatoid , Case-Control Studies , Microbiota/immunology , Bacteria , Epidemiologic Studies , Data Interpretation, Statistical , Indonesia/epidemiology
19.
Article in English | LILACS, BBO | ID: biblio-1135523

ABSTRACT

Abstract Objective: To investigate the effect of deploying a "First Aid Management of Dental Avulsion" poster in a primary school setting to improve teachers' knowledge. Material and Methods: The present study was conducted at public primary schools in central Jakarta, with 54 teachers meeting the inclusion criteria. The questionnaire contained 13 questions, divided into two sections. The first section consisted of questions about sex, age, education, length of service, field of education, and whether the teacher had received first aid management education before. The second section consisted of 7 questions about the teacher's knowledge regarding dental avulsion and its management. Baseline knowledge was defined by using a questionnaire and then teachers were shown the educational poster covering the first aid management of dental avulsion. Next, the teachers answered a post-education questionnaire, which included the same questions as the first questionnaire. A paired t-test or Wilcoxon's signed-rank test was used based on the distribution of data to determine the significance of the differences between before and after education. Results: The median score before reading the poster was 5, while the median score after reading it was 10. The difference was statistically significant. Conclusion: "First Aid Management of Dental Avulsion" poster could improve the knowledge of primary school teachers in Jakarta.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Tooth Avulsion/diagnosis , Health Knowledge, Attitudes, Practice , Tooth Injuries/prevention & control , First Aid , School Teachers , Surveys and Questionnaires , Statistics, Nonparametric , Indonesia/epidemiology
20.
Article in English | LILACS, BBO | ID: biblio-1135520

ABSTRACT

Abstract Objective: To determine diametral tensile strength and water absorption ability of Carbonate-apatite blocks fabricated from gypsum precursors (CaSO4) through a dissolution-precipitation method, with different solution molarities and immersion times. Material and Methods: Thirty-six CaSO4 gypsum specimens with 6 mm diameter; 3 mm height (Group A for diametral tensile strength) and 36 specimens of 6 mm diameter; 3 mm thickness (Group B for water absorption ability) were used. Each group was divided into 4 group treatments of: 1) dissolution-precipitation in solution of 0.5mol / L Na2CO3 + 0.5mol / L Na3PO4 for 48 hours and 72 hours; 1mol / L Na2CO3 + 1mol / L Na3PO4 for 48 hours and 72 hours. The C-Ap blocks were then tested using ATR-FTIR spectrometer to identify the formation of C-Ap functional groups. Furthermore, Group A specimens were tested for diametral tensile strength using Universal Testing Machine, and Group B specimens were tested for water adsorption ability using an analytical balance by measuring initial and final weight after immersion in saline solution at 37oC for 24 hours. Results: The formation of carbonate-apatite (C-Ap) in groups with solution molarity of 1 mol/L for 48 hours and 72 hours resulted in a lower diametral tensile strength and water absorption ability than the groups with a 0.5 mol/L solution. Conclusion: Solution with different molarities and dissolution-precipitation duration affect the formation of carbonate-apatite blocks.


Subject(s)
Tensile Strength , Calcium Sulfate/therapeutic use , Adsorption , Dental Materials , Analysis of Variance , Spectroscopy, Fourier Transform Infrared/instrumentation , Statistics, Nonparametric , Indonesia/epidemiology
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