Stem as a direct source of rauwolfia radix

Rauwolfia radix was produced both via callus and directly, using stem as an explant. MS medium containing 1 mg/1 NAA and 0.5 mg/1 Kn with 1% CH was required for proliferation of callus and initiation of root projections [visible root primordial]. Continuous [24 h] illumination was required for development of root projections into true roots. Roots formed via callus or direct on stem explant were distinguishable from each other. WRC medium was employed for root propagation, with addition of 0.8 mg/l NAA and 10 mg/l biotin

Tissue culture studies on Australian solanum [S. aviculare]

Callus cultures of Solanum aviculare forst. were established from cotyledons, leaves, stem and root segments. Callus was propagated successfully on Abou-Mandour's medium. It remained undifferentiated except for root differentiation, observed in stem and root calli

Tissue culture studies on American solanum [S. mammosum L.]

Callus cultures of Solanum mammosum L. were established for stem, leaf and root segments. Callus was propagated successfully on Abou-Mandour's [1977] and Murashige and Skoog's [1962] media. Undifferentiated callus growth active for 22 weeks was observed with varying concentrations of 2,4-D and BAP respectively in AM and MS media

Differentiation, de-differentiation and re-differentiation of roots in rauwolfia leaf callus

In vitro Rauwolfia roots were differentiated via regenerated plant's leaf callus cultures, grown on WRC medium containing 10% CM and 0.1 mg/l each of NAA and BAP. The roots de-differentiated in part into callus, which in turn re-differentiated roots and the whole process was repeated by Rauwolfia cultures on a regular basis

Shoot regeneration in rauwolfia root callus

Rauwolfia buds were cultured in vitro for root initiation. Roots were excised, cultured and callused with 1 mg/I IAA+10 mg/I BAP. Callus was propagated successfully using different treatments. Shoot regeneration was induced on MS medium supplemented with 10% CM and 5 mg/I biotin

Bud multiplication in Juniper

Excised juniper multiplied in 2 weeks with 0.7% polyvinylpyrrolidone [PVP], 2% sucrose and 0.5 mg/l BAP. Hundred% multiplication was observed in the cultured buds. A maximum number of new buds [multiplication product] were obtained with 1 mg/l K, i.e. 9 buds per single treated bud

Tissue culture of ajowan-e-khurasani [hymoscyamus niger L]

Callus cultures were initiated from redical, leaf, petiole, stem and root explants of H. niger. MS medium with kinetin and 2,4-D at a concentration of 0.1 mg/1 was found suitable for good amount of callus formation. Callus was propagated in undifferentiated form, for more than a year with the addition of CH, CM and SDC to the culture medium. The presence of alkaloids in cultures was observed dependent on the colour and origin of the callus