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1.
Braz. arch. biol. technol ; 63: e20190216, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132175

ABSTRACT

Abstract In the last years phytosterols, natural components of plants, have received more attention due to association of their consumption with reducing risk of cardiovascular diseases and cancer. There are several scientific studies about phytosterols in vegetable oils, but they are scarce in unconventional oils. The objective of this research was evaluating the content of phytosterols (β-sitosterol, stigmasterol and campesterol) in vegetable oils sold in São Paulo city, in Brazil. The analysis included cold alkaline saponification, derivatization with hexamethyldisilazane and trimethylchlorosilane reagents, and quantification by gas chromatography using flame ionization detection and internal standardization. The quality control parameters indicated that the method was suitable for analysis. Total sterols were between 272.3 mg kg-1 (coconut oil) to 6169.7 mg kg-1 (evening primrose oil). β-sitosterol was the component found in higher concentrations and evening primrose oil was the most representative in quantity of phytosterols.


Subject(s)
Phytosterols/analysis , Plant Oils/analysis , Sitosterols/analysis , Stigmasterol/analysis , Plant Oils/classification , Brazil , Chromatography, Gas , Flax , Carthamus tinctorius , Palm Oil/analysis
2.
Acta Pharmaceutica Sinica ; (12): 599-604, 2015.
Article in Chinese | WPRIM | ID: wpr-257094

ABSTRACT

1H NMR-based metabolomic approach combined with multivariate statistical analysis was used to evaluate the quality of 21 Farfarae Flos (FF) samples from different growth regions. Principal component analysis showed that wild and cultivated FF could be separated clearly, suggesting a big chemical difference existed between them. Supervised PLS-DA analysis indicated that the wild samples showed higher levels of secondary metabolites, such as bauer-7-ene-3β, 16α-diol, chlorogenic acid, rutin, 7-(3'-ethylcrotonoyloxy)-1α-(2'-methyl-butyryloxy)-3, 14-dehydro-Z-notonipetranone (EMDNT), tussilagone, β-sitosterol and sitosterone. This is consistent with traditional experience that the quality of wild samples are better than that of cultivated ones. The content of pyrrolizidine alkaloids senkirkine also differed greatly among samples from different habitats. The Pearson correlation analysis showed that senkirkine is positively correlated with 4, 5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, rutin, kampferol analogues, to a statistically significant extent. The correlation between the toxic compounds and the bioactive components in FF should be further studied.


Subject(s)
Chlorogenic Acid , Drugs, Chinese Herbal , Chemistry , Flowers , Chemistry , Magnetic Resonance Imaging , Magnetic Resonance Spectroscopy , Metabolomics , Quinic Acid , Rutin , Sitosterols , Tussilago , Chemistry
3.
Acta Pharmaceutica Sinica ; (12): 70-74, 2015.
Article in Chinese | WPRIM | ID: wpr-251815

ABSTRACT

The chemical constituents of Taxus chinensis var. mairei cell cultures were investigated by chromatographic methods, including silica gel column chromatography, Sephadex LH-20 and preparative HPLC. Thirteen compounds were isolated from the 80% ethanol extract of cultured cells and their structures were elucidated by spectral data and physicochemical properties, which were identified as 2α,4α,7β,9α,10β-pentaacetoxy-14β-hydroxytax-11-ene (1), 2α,4α,7β,9α,10β-pentaacetoxytax-11-ene (2), 1β-deoxybaccatin VI (3), 2α-acetoxytaxusin (4), taxuyunnanine C (5), yunnanxane (6), 2α,5α,10β-triacetoxy-14β-propionyloxy-4 (20), 11-taxadiene (7), 2α,5α,10β-triacetoxy-14β-isobutyryloxy-4 (20), 11-taxadiene (8), 2α,5α,10β-triacetoxy-14β-(2'-methyl)butyryloxy-4 (20), 11-taxadiene (9), 13-dehydroxylbaccatin III (10), 13-dehydroxy-10-deacetylbaccatin III (11), paclitaxel (12) and (13) β-sitosterol. Among them, compound 1 is a new compound, and compounds 2, 4, 10 and 11 are isolated from the cell culture of Taxus chinensis var. mairei for the first time.


Subject(s)
Alkenes , Cell Culture Techniques , Cells, Cultured , Diterpenes , Molecular Structure , Paclitaxel , Sitosterols , Taxoids , Taxus , Chemistry
4.
Indian J Exp Biol ; 2014 Jan; 52(1): 60-66
Article in English | IMSEAR | ID: sea-150333

ABSTRACT

High fat diet group showed a significant rise in serum and hepatic total cholesterol, triglyceride and atherogenic index which are major biomarkers of dyslipidemia and cardiovascular risk. The liver function markers, lipid peroxidation and proinflammatory cytokine levels were elevated in high fat diet group whereas antioxidant levels significantly reduced. These findings manifest hepatic damage which was further confirmed by histological findings. Quercetin and β-sitosterol though structurally different yet both ameliorate the sickening changes in different mechanism. The current investigation is perhaps the first report of the mechanistic role of two polyphenols over dyslipidemia and subsequent hepatotoxicity.


Subject(s)
Animals , Antioxidants/metabolism , Cardiovascular Diseases/blood , Cardiovascular Diseases/metabolism , Cardiovascular Diseases/pathology , Diet, High-Fat , Dyslipidemias/drug therapy , Dyslipidemias/pathology , Lipid Peroxidation/drug effects , Lipids/blood , Liver/drug effects , Mice , Quercetin/administration & dosage , Sitosterols/administration & dosage , Triglycerides/blood
5.
Article in Chinese | WPRIM | ID: wpr-305372

ABSTRACT

In order to establish a method for the determination of the sterols of the oil in the freeze-dried acai (Euterpe oleracea Mart.) and to evaluate its antioxidant activities, a saponification/extraction procedure and high performance liquid chromatography (HPLC) analysis method were developed and validated for the analysis of phytosterols in PEE (Petroleum ether extract). Separation was achieved on a Purosper STAR LP C18 column with a binary, gradient solvent system of acetonitrile and isopropanol. Evaporative light scattering detection (ELSD) was used to quantify β-sitosterol and the total sterols. Peak identification was verified by retention times and spikes with external standards. Standard curves were constructed (r = 0.999 2) to allow for sample quantification. Recovery of the saponification and extraction was demonstrated via analysis of spiked samples. The highest content of total sterols is β-sitosterol. The antioxidant activities of the extracts were evaluated using the total oxyradical scavenging capacity assay (TOSC assay). The result showed that the PEE exhibited significant antioxidant properties, sample concentration and the antioxidant capacity had a certain relevance.


Subject(s)
Antioxidants , Arecaceae , Chemistry , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Phytosterols , Sitosterols
6.
Article in Chinese | WPRIM | ID: wpr-341811

ABSTRACT

Column chromatography on silica gel was used to study the chemical constituents of traditional Chinese medicine Siegesbeckia pubescens. The chemical structures of the separated compounds were elucidated by spectroscopic data analyses. As a result, eighteen compounds were obtained and identified as 3, 4'-dimethoxy quercetin(1), 3, 3', 4'-trimethoxy quercetin(2), 3, 3'-dimethoxy quercetin(3), 7, 3', 4'-trimethoxy luteolin(4), ursolic acid(5), 2β,19α-dihydroxyursolic acid(6), 2β-hydroxyursolic acid (7), stigmasterol-7-one(8), 5α, 8α-epidioxy-24(R)-methyl-cholesta-6, 22-diene-3β-ol(9), β-sitosterol(10), 2, 6-di(3-hydroxy-4-methoxyphenyl)-3, 7-dioxacyclo [3. 3. 0] octane (11), aurantiamide acetate (12), 3-(m-hydroxyl-p-methoxy)-N-(2'-p-hydroxyl-phenethyl)-2E-acrylamide(13), p-hydroxy benzaldehyde (14), m-hydroxy-p-methoxy benzaldehyde (15), 3, 4, 5-trimethoxybenzoic acid(16), monoethyl malonate(17), and p-hydroxylcinnamic acid(18). Among them, compounds 1-9, 11-18 were isolated from this plant for the first time.


Subject(s)
Asteraceae , Chemistry , Drugs, Chinese Herbal , Chemistry , Luteolin , Chemistry , Medicine, Chinese Traditional , Plants, Medicinal , Quercetin , Chemistry , Sitosterols , Chemistry , Triterpenes , Chemistry
7.
Article in Chinese | WPRIM | ID: wpr-321379

ABSTRACT

<p><b>OBJECTIVE</b>To explore the feasibility of preparing novel gypenosides long-circulating liposomes with PEG grafted on beta-sitosterol (PEG-Sito).</p><p><b>METHOD</b>Succinicanhydride was adopted to connect beta-sitosterol and PEG 2000. Sphingomyelin and PEG-Sito was used as material to prepare gypenosides long-circulating liposomes by using ethanol injection method. Encapsulation efficiency was determined by using protamine precipitation method. H-NMR was used to verify the synthesis of PEG-Sito, the novel gypenosides long-circulating liposomes were characterized by particle size, zeta potential and atomic force microscope.</p><p><b>RESULT</b>The synthesis of PEG-Sito was verified by 1H-NMR. Encapsulation efficiency of long-circulating liposomes prepared by ethanol injection method was 74.3%, particle size was 288.1 nm, zeta potential was -20.25 mV, the morphology were round observed by AFM.</p><p><b>CONCLUSION</b>The novel gypenosides long-circulating liposomes prepared with PEG-Sito was feasible, it had a high encapsulation efficiency and good morphology.</p>


Subject(s)
Drug Compounding , Methods , Feasibility Studies , Gynostemma , Chemistry , Liposomes , Blood , Chemistry , Plant Extracts , Chemistry , Polyethylene Glycols , Chemistry , Reproducibility of Results , Sitosterols , Chemistry , Sphingomyelins , Chemistry
8.
Journal of Medicinal Plants. 2014; 13 (49): 15-21
in English | IMEMR | ID: emr-141782

ABSTRACT

Echinophora platyloba DC [Apiaceae] is one of the two endemic species of Echinophora genus in Iran. It has many folkloric uses and several biological activities but there is no information about its phytoconstituents. In this work some of the secondary metabolites were isolated, purified and identified from n-hexane, ethyl acetate and methanol extracts from the aerial parts and root of this plant for the first time. Resulting extracts were subjected to column chromatography using EtOAc/n-hexane as eluent. Further purifications were carried out using preparative TLC and recrystallization techniques. Characterization of compounds were established using spectroscopic data [[1]H-NMR, [13]C-NMR, MS and FT-IR], and by comparing with the literature. Stigmasterol, Sitosterol, Stigmasterol-beta-D-glycoside and Saccharose were purified from these extracts and their chemical structures were characterized. Several phytosterols were isolated from various extracts of aerial parts and great amount of saccharose was purified from the sweet root of the plant


Subject(s)
Plant Extracts , Sucrose , Stigmasterol , Sitosterols
9.
Indian J Exp Biol ; 2013 Mar; 51(3): 241-248
Article in English | IMSEAR | ID: sea-147588

ABSTRACT

With a view to investigate the ameliorative effects of sitosterol esters against degenerative effects of hypercholesterolemia brain antioxidant enzyme assays, brain lipid profile, brain phospholipid compositional change and brain neurotransmitter concentrates (glutamic acid, asparctic acid, glycine) were measured in hypercholesterolemic rats. The results indicated that phytosterol esters have a role in countering hypercholesterolemia-related changes in the brain by decreasing the cholesterol levels, increasing the phospholipid levels and increasing the level of antioxidant enzymes. The results suggest that phytosterol esters may be of therapeutic significance and may offer new and effective options for the treatment of hypercholesterolemia-induced changes in the brain.


Subject(s)
Amino Acids/metabolism , Animals , Antioxidants/metabolism , Brain/drug effects , Brain/metabolism , Chromatography , Docosahexaenoic Acids/metabolism , Eicosapentaenoic Acid/metabolism , Esters/pharmacology , Fish Oils/metabolism , Glutathione/metabolism , Hypercholesterolemia/metabolism , Male , Neurotransmitter Agents/metabolism , Phytosterols/pharmacology , Rats , Rats, Wistar , Sitosterols/pharmacology , alpha-Linolenic Acid/metabolism
10.
Article in Chinese | WPRIM | ID: wpr-294072

ABSTRACT

Sixteen compounds were isolated from Conioselinum vaginatum by silica column chromatography over silica gel and Sephadex LH-20, as well as recrystallization. On the basis of their physical and chemical properties and spectral data, their structures were identified as ligustilide (1), 1,3-dilinolein (2), coniferaldehyde (3), myristicin (4), stigmasterol (5), beta-sitosterol (6), vanillin (7), pregnenolone (8), bergapten (9), xanthotoxin (10), methyl indole-3-carboxylate (11), ferulic acid (12), (E)-3-methoxy-4,5-methylenedioxy-cinnamic alcohol (13), p-hydroxybenzaldehyde (14), 3-methoxy-4,5-methylenedioxy-acetophenone (15), and alpha-(ethoxymethyl)-4-hydroxy- benzenemethanol (16). Among them, compound 15 was a new natural product, and compounds 2, 3, 10, 11, 14, and 16 were obtained from this genus for the first time.


Subject(s)
Apiaceae , Chemistry , Benzaldehydes , Chemistry , Dextrans , Chemistry , Drugs, Chinese Herbal , Chemistry , Mass Spectrometry , Molecular Structure , Sitosterols , Chemistry
11.
Article in English | WPRIM | ID: wpr-632948

ABSTRACT

Moist exposed burn therapy is a therapeutic principle based on the moist environment of the wound, using an ointment of herbal origin that enhances epithelial repair. This is the case of a 42-year-old female who sustained partial thickness burn wounds on her face and upper extremities. Patient was started on wound dressing with 13-sitosterol and berberine moist exposed burn ointment (MEBO) and noted the formation of erythematous verrucous plaques overlying the burned areas after four weeks. Histopathological examination of the lesions revealed papillomatosis, acanthosis, and hyperkeratosis. The lesions resolved upon discontinuation of MEBO and application of a combination of topical steroid, keratolytic, and emollient. The reactive epidermal hyperplasia seen in the case presented may have been due to increased levels of basic fibroblast growth factor in burn wounds treated with MEBO. Careful instructions and close monitoring of patients applying the ointment must be reinforced due to the possibility of reactive inflammatory skin reactions.


Subject(s)
Humans , Female , Middle Aged , Bandages , Berberine , Burns , Emollients , Fibroblast Growth Factor 2 , Hyperplasia , Ointments , Sitosterols , Wound Healing
12.
Article in Chinese | WPRIM | ID: wpr-263883

ABSTRACT

<p><b>OBJECTIVE</b>To investigate chemical constituents from folk herb Pilea cavaleriei subsp. cavaleriei.</p><p><b>METHOD</b>The compounds were separated and purified by silica gel, Sephadex LH-20 and the like. The structures were identified by spectral methods such as (1)H, (13)C-NMR and MS.</p><p><b>RESULT</b>Seventeen compounds were isolated and identified as benzoic acid (1), 4-hydroxy benzalde-hyde (2), coumaric acid(3), protocatechuic acid (4), gallic acid (5), 4-hydroxy benzoic acid (6), 3-indole carboxaldehyde (7), 3-indole carbo-xylicacid (8), 4-methyl-(1,2,3) -triazole(9), uracil(10), nicotinamide (11), (2S,E)-N-[2-hydroxy-2-(4-hydroxy phenyl) ethyl] ferulamide (12), (+) -dehydrovomifoliol (13), hentriantane (14), beta-sitosterol (15), palmitic acid (16), daucossterol (17) , respectively.</p><p><b>CONCLUSION</b>All compounds were obtained from the genus for the first time.</p>


Subject(s)
Dextrans , Chemistry , Drugs, Chinese Herbal , Chemistry , Molecular Structure , Sitosterols , Chemistry , Urticaceae , Chemistry
13.
Article in English | WPRIM | ID: wpr-154545

ABSTRACT

Pumpkins have considerable variation in nutrient contents depending on the cultivation environment, species, or part. In this study, the general chemical compositions and some bioactive components, such as tocopherols, carotenoids, and beta-sitosterol, were analyzed in three major species of pumpkin (Cucurbitaceae pepo, C. moschata, and C. maxima) grown in Korea and also in three parts (peel, flesh, and seed) of each pumpkin species. C. maxima had significantly more carbohydrate, protein, fat, and fiber than C. pepo or C. moschata (P < 0.05). The moisture content as well as the amino acid and arginine contents in all parts of the pumpkin was highest in C. pepo. The major fatty acids in the seeds were palmitic, stearic, oleic, and linoleic acids. C. pepo and C. moschata seeds had significantly more gamma-tocopherol than C. maxima, whose seeds had the highest beta-carotene content. C. pepo seeds had significantly more beta-sitosterol than the others. Nutrient compositions differed considerably among the pumpkin species and parts. These results will be useful in updating the nutrient compositions of pumpkin in the Korean food composition database. Additional analyses of various pumpkins grown in different years and in different areas of Korea are needed.


Subject(s)
Arginine , beta Carotene , Carotenoids , Cucurbita , Fatty Acids , gamma-Tocopherol , Korea , Linoleic Acid , Linoleic Acids , Nutritive Value , Seeds , Sitosterols , Tocopherols
14.
Article in Chinese | WPRIM | ID: wpr-267041

ABSTRACT

Twelve compounds were separated from stems of Dysoxylum laxiracemosum and their structures were identified by spectrum analysis as shoreic acid (1), cabraleahydroxylactone (2), cabralealactone (3), cinchonain (5), catechin (6), scopoletin (7), vanillic acid (8), p-hydroxybenzoic acid (9), docosanol (10), beta-sitosterol (11), daucosterol (12). Of them, compounds 1-6,8-12 were separated from this plant for the first time, and compounds 4-6 were reported from this plant genus for the first time.


Subject(s)
Catechin , Chemistry , Meliaceae , Chemistry , Plant Stems , Chemistry , Scopoletin , Chemistry , Sitosterols , Chemistry , Vanillic Acid , Chemistry
15.
Article in Chinese | WPRIM | ID: wpr-338697

ABSTRACT

Twenty-one non-anthraquinones constituents were isolated for the first time from an ethanol extract of the roots of Knoxia valerianoides by using a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, and reversed-phase HPLC. Their structures were identified by their physical-chemical properties and spectroscopic analysis including NMR and MS. The compounds include ten triterpenoids: ursolic acid (1), oleanolic acid (2), 2-oxo pomolic acid (3), pomolic acid (4), maslinic acid (5), rotungenic acid (6), tormentic accid (7), rotundic acid 3,23-acetonide (8), arjungenin (9), and 2alpha, 3beta, 19alpha, 23-tetrahydroxy-urs-12-en-28-oic acid (10), four sitosterones: (24R)-24-ethylcholesta-4,22-dien-3-one (11), 3-oxo-4-en-sitosterone (12), 7-oxostigmasterol (13), and 7-oxo-beta-sitosterol (14), two lignans: eudesmin (15) and ciwujiatone (16), one coumarin: cnidilin (17), and four simple aromatic analogues: 5-hydroxymethylenefural (18), 3-hydroxy-4-methoxybenzoic acid (19), benzoic acid (20), and 2-hydroxy-5-methxoycinnamaldehydes (21). In the in vitro assays against human cancer cell lines (HCT-8, Bel7402, BGC-823, A549, and A2780), against deserum and glutamate induced PC12-syn cell damage, and against HIV-1 replication, and inhibiting protein tyrosine phosphatase 1B (PTP1 B), LPS induced NO production in macrophage, and Fe(2+)-cystine induced rat liver microsomal lipid peroxidation, at a concentration of 1 x 10(-5) mol x L(-1), no compound showed activity.


Subject(s)
Animals , Cell Line, Tumor , Chromatography, High Pressure Liquid , Humans , Lignans , Chemistry , Mice , Plant Roots , Chemistry , Rubiaceae , Chemistry , Sitosterols , Chemistry , Pharmacology , Triterpenes , Chemistry , Pharmacology
16.
Article in English | WPRIM | ID: wpr-819794

ABSTRACT

OBJECTIVE@#To study the isolation and characterization of the constituent responsible for the cytotoxic activity of the ethanolic extract of stem of Capparis decidua (C. decidua).@*METHODS@#The preliminary cytotoxic effect of isolated compound (β-Sitosterol triacontenate) was investigated by MTT assay on A549 solid tumor cells.@*RESULTS@#IC(50) value of the β-Sitosterol triacontenate was found to be 1 μM. The cytotoxic activity increased in a dose dependent manner in case of β-Sitosterol triacontenate.@*CONCLUSIONS@#The data therefore provide direct evidence for the role of β-Sitosterol triacontenate as a potent antimetastatic agent, which can markedly inhibit the metastatic and invasive capacity of malignant cells.


Subject(s)
Antineoplastic Agents , Chemistry , Pharmacology , Capparis , Chemistry , Cell Line, Tumor , Enzyme-Linked Immunosorbent Assay , Humans , Lung Neoplasms , Drug Therapy , Magnetic Resonance Spectroscopy , Neoplasm Invasiveness , Neoplasm Metastasis , Plant Extracts , Pharmacology , Plant Stems , Chemistry , Sitosterols , Chemistry , Pharmacology
17.
Article in Chinese | WPRIM | ID: wpr-356039

ABSTRACT

<p><b>OBJECTIVE</b>To study the chemical constituents from the seeds of Notopterygium franchetii.</p><p><b>METHOD</b>Ethanol extracts of seeds N. franchetii were separated and purified by such methods as normal and reversed phase column chromatographies and thin-layer chromatography and structurally elucidated by MS and NMR evidences.</p><p><b>RESULT</b>Twenty nine compounds were separated, they were isoimperatorin (1), [3-sitosterol (2), phellopterin (3), bergapten (4), N-tetra, hexa, octacosanoylanthranilic acid (5-7), daucosterol (8), oxypeucedanin hydrate (9), umbelliferone (10), demethylfuropinnarin (11), (2S, 3S, 4R, 8E)-2-[(2'R)- 2'-hydroxydoco, trico, tetraco, entaco, hexaco sanosylamino] -octadecene-1, 3, 4-triol (12-16), (-)-oxypeucedanin (17), diosmetin (18), bergaptol-O-beta-D-glucopyranoside (19), nodakenin (20), 1'-O-beta-D-glucopyranosyl-(2R, 3S)-3-hydroxynodakenetin (21), uracil (22), decuroside V (23), 8-O-beta-D-glucopyranosyl-5-hydroxypsoralen (24), 8-O-beta-D-glucopyranosyl-5-methoxylpsoralen (25), diosmin (26), alaschanioside C (27), kynurenic acid (28) and mannitol (29).</p><p><b>CONCLUSION</b>All of these compounds were separated from the seeds of N. franchetii for the first time. Of them, 18, 22, 26 and 29 were firstly obtained from genus Notopterygium.</p>


Subject(s)
Apiaceae , Chemistry , Chromatography, Thin Layer , Coumarins , Chemistry , Diosmin , Chemistry , Flavonoids , Chemistry , Furocoumarins , Chemistry , Glucosides , Chemistry , Kynurenic Acid , Chemistry , Magnetic Resonance Spectroscopy , Mannitol , Chemistry , Methoxsalen , Chemistry , Seeds , Chemistry , Sitosterols , Chemistry , Uracil , Chemistry
18.
Article in Chinese | WPRIM | ID: wpr-356038

ABSTRACT

<p><b>OBJECTIVE</b>To study sesquiterpenoids of Coniogramme maxima.</p><p><b>METHOD</b>Chemical constituents were separated by chromatography and their structures were identified according to physicochemical property and spectrum data.</p><p><b>RESULT</b>Fifteen compounds were separated by chromatography technique. Their structures were determined by spectral data, including 10 sesquiterpenoids as (3S)-pteroside D (1), epi-pterosin L (2), pterosin D (3), onitin (4), pterosin Z (5), onitisin (6), onitisin-glucopyranoside (7), onitin-15-O-beta-D-glucopyranoside (8), (2S,3R)-pterosin-L-2'-O-beta-D-glucopyranoside (9) and (3R)-peterosin D-3-O-beta-D-glucopyranoside (10). The other compounds were uracil (11), 3,4-dihydroxybenzaldehyde (12), 5-hydroxymethyl-2-furancarboxaldehyde (13), beta-sitosterol (14) and daucosterol (15).</p><p><b>CONCLUSION</b>The above 15 compounds are separated from C. maxima for the first time, including 9 compounds being first separated from genus Coniogramme.</p>


Subject(s)
Ferns , Chemistry , Indans , Chemistry , Indenes , Chemistry , Sesquiterpenes , Chemistry , Sitosterols , Chemistry
19.
An. acad. bras. ciênc ; 83(4): 1165-1170, Dec. 2011. ilus, tab
Article in English | LILACS | ID: lil-607420

ABSTRACT

Plumbago scandens L. is a Brazilian tropical/subtropical species that occurs along the coast. Chemically it is mainly represented by naphthoquinones, flavonoids, terpenoids and steroids. The aim of the present work is to study quantitative changes in the root metabolic production of Plumbago scandens during different physiologic developmental stages relative to floration. The results indicated the presence of four substances in the extracts: plumbagin, epi-isoshinanolone, palmitic acid and sitosterol, independent on developmental stage. The naphthoquinone plumbagin has always showed to be the major component of all extracts. Naphthoquinones exhibited their highest content during floration, while the content of the two others components decreased during this stage, revealing an inverse profile. The chemical composition changed depending on the plant requirements.


Plumbago scandens L. é uma espécie brasileira tropical/subtropical que ocorre ao longo da costa. Quimicamente, é principalmente representada por naftoquinonas, flavonóides, terpenóides e esteróides. objetivo do presente trabalho é estudar mudanças quantitativas da produção metabólica nas raízes de Plumbago scandens durante diferentes estágios de desenvolvimento fisiológico, relativos à floração. Os resultados indicaram a presença de quatro substâncias nos extratos: plumbagina, epi-isoshinanolona, ácido palmítico e sitosterol, independente do estágio de desenvolvimento. A naftoquinona plumbagina tem sempre mostrado ser o componente majoritário de todos os extratos. Naftoquinonas exibiram seus maiores conteúdos durante a floração, enquanto o conteúdo dos dois outros componentes decresceu durante este estágio, revelando um perfil inverso. A composição química modificou dependendo das necessidades da planta.


Subject(s)
Naphthoquinones/chemistry , Palmitic Acid/chemistry , Plant Roots/chemistry , Plumbaginaceae/chemistry , Sitosterols/chemistry , Tetrahydronaphthalenes/chemistry , Chromatography, Gas , Naphthoquinones/metabolism , Palmitic Acid/metabolism , Plant Roots/growth & development , Plant Roots/metabolism , Plumbaginaceae/growth & development , Plumbaginaceae/metabolism , Sitosterols/metabolism , Tetrahydronaphthalenes/metabolism
20.
Article in Chinese | WPRIM | ID: wpr-247389

ABSTRACT

<p><b>OBJECTIVE</b>To identify the endophyte strain E8 with high activity from Curcuma wenyujin and study its secondary metabolites.</p><p><b>METHOD</b>The strain E8 was identified by morphological observation and ITS sequence analysis. Manifold chromatographic methods were used to separate and purify the chemical constituents of fermentation broth from strain E8, and their structures were identified by physiochemical properties and spectral data.</p><p><b>RESULT</b>The strain E8 belongs to P. oxalicum. Four compounds were isolated from the fermentation broth of this strain and elucidated as chrysophanol, emodin, secalonic acid A and beta-sitosterol.</p><p><b>CONCLUSION</b>The endophyte P. oxalicum was isolated from medicinal plant Curcuma wenyujin for the first time. Four compounds were first isolated from endophytic fungus in C. wenyujin. Thus, microbial fermentation is a new access for these compounds production.</p>


Subject(s)
Anthraquinones , Curcuma , Microbiology , Emodin , Fermentation , Penicillium , Genetics , Metabolism , Sitosterols , Xanthones
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